Acidipropionibacterium virtanenii sp. nov., isolated from malted barley

Paulina Deptula (Corresponding Author), Olli Pekka Smolander, Pia Laine, Richard J. Roberts, Minnamari Edelmann, Petri Peltola, Vieno Piironen, Lars Paulin, Erna Storgards, Kirsi Savijoki, Arja Laitila, Petri Auvinen, Pekka Varmanen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

Abstract

A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278T to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278T is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37° C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278T (=VTT E-113202T =DSM 106790T ).

Original languageEnglish
Pages (from-to)3175-3183
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number10
DOIs
Publication statusPublished - 29 Aug 2018
MoE publication typeNot Eligible

Fingerprint

Hordeum
barley
genome
Genome
rRNA Genes
Catalase
new species
Propionibacterium
gene
Base Composition
Finland
Fermentation
fermentation
Names
phenotype
Carbon
Nucleotides
catalase
Propionibacterium acidipropionici
Phenotype

Keywords

  • A
  • Acidipropionibacterium virtanenii
  • Acidipropionici
  • Defence mechanisms
  • Jensenii
  • Malted barley
  • Pseudovitamin b12

Cite this

Deptula, P., Smolander, O. P., Laine, P., Roberts, R. J., Edelmann, M., Peltola, P., ... Varmanen, P. (2018). Acidipropionibacterium virtanenii sp. nov., isolated from malted barley. International Journal of Systematic and Evolutionary Microbiology, 68(10), 3175-3183. https://doi.org/10.1099/ijsem.0.002965
Deptula, Paulina ; Smolander, Olli Pekka ; Laine, Pia ; Roberts, Richard J. ; Edelmann, Minnamari ; Peltola, Petri ; Piironen, Vieno ; Paulin, Lars ; Storgards, Erna ; Savijoki, Kirsi ; Laitila, Arja ; Auvinen, Petri ; Varmanen, Pekka. / Acidipropionibacterium virtanenii sp. nov., isolated from malted barley. In: International Journal of Systematic and Evolutionary Microbiology. 2018 ; Vol. 68, No. 10. pp. 3175-3183.
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title = "Acidipropionibacterium virtanenii sp. nov., isolated from malted barley",
abstract = "A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 {\%}, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278T to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278T is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol{\%}. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37° C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278T (=VTT E-113202T =DSM 106790T ).",
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Deptula, P, Smolander, OP, Laine, P, Roberts, RJ, Edelmann, M, Peltola, P, Piironen, V, Paulin, L, Storgards, E, Savijoki, K, Laitila, A, Auvinen, P & Varmanen, P 2018, 'Acidipropionibacterium virtanenii sp. nov., isolated from malted barley' International Journal of Systematic and Evolutionary Microbiology, vol. 68, no. 10, pp. 3175-3183. https://doi.org/10.1099/ijsem.0.002965

Acidipropionibacterium virtanenii sp. nov., isolated from malted barley. / Deptula, Paulina (Corresponding Author); Smolander, Olli Pekka; Laine, Pia; Roberts, Richard J.; Edelmann, Minnamari; Peltola, Petri; Piironen, Vieno; Paulin, Lars; Storgards, Erna; Savijoki, Kirsi; Laitila, Arja; Auvinen, Petri; Varmanen, Pekka (Corresponding Author).

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 10, 29.08.2018, p. 3175-3183.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Acidipropionibacterium virtanenii sp. nov., isolated from malted barley

AU - Deptula, Paulina

AU - Smolander, Olli Pekka

AU - Laine, Pia

AU - Roberts, Richard J.

AU - Edelmann, Minnamari

AU - Peltola, Petri

AU - Piironen, Vieno

AU - Paulin, Lars

AU - Storgards, Erna

AU - Savijoki, Kirsi

AU - Laitila, Arja

AU - Auvinen, Petri

AU - Varmanen, Pekka

PY - 2018/8/29

Y1 - 2018/8/29

N2 - A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278T to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278T is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37° C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278T (=VTT E-113202T =DSM 106790T ).

AB - A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278T to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278T is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37° C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278T (=VTT E-113202T =DSM 106790T ).

KW - A

KW - Acidipropionibacterium virtanenii

KW - Acidipropionici

KW - Defence mechanisms

KW - Jensenii

KW - Malted barley

KW - Pseudovitamin b12

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U2 - 10.1099/ijsem.0.002965

DO - 10.1099/ijsem.0.002965

M3 - Article

VL - 68

SP - 3175

EP - 3183

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

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ER -