Acidipropionibacterium virtanenii sp. nov., isolated from malted barley

Paulina Deptula (Corresponding Author), Olli Pekka Smolander, Pia Laine, Richard J. Roberts, Minnamari Edelmann, Petri Peltola, Vieno Piironen, Lars Paulin, Erna Storgards, Kirsi Savijoki, Arja Laitila, Petri Auvinen, Pekka Varmanen (Corresponding Author)

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278T to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278T is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37° C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278T (=VTT E-113202T =DSM 106790T ).

    LanguageEnglish
    Pages3175-3183
    Number of pages9
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume68
    Issue number10
    DOIs
    Publication statusPublished - 29 Aug 2018
    MoE publication typeNot Eligible

    Fingerprint

    Hordeum
    barley
    genome
    Genome
    rRNA Genes
    Catalase
    new species
    Propionibacterium
    gene
    Base Composition
    Finland
    Fermentation
    fermentation
    Names
    phenotype
    Carbon
    Nucleotides
    catalase
    Propionibacterium acidipropionici
    Phenotype

    Keywords

    • A
    • Acidipropionibacterium virtanenii
    • Acidipropionici
    • Defence mechanisms
    • Jensenii
    • Malted barley
    • Pseudovitamin b12

    OKM Publication Types

    • A1 Refereed journal article

    OKM Open Access Status

    • 0 Not Open Access

    ASJC Scopus subject areas

    • Microbiology
    • Ecology, Evolution, Behavior and Systematics

    Cite this

    Deptula, P., Smolander, O. P., Laine, P., Roberts, R. J., Edelmann, M., Peltola, P., ... Varmanen, P. (2018). Acidipropionibacterium virtanenii sp. nov., isolated from malted barley. International Journal of Systematic and Evolutionary Microbiology, 68(10), 3175-3183. https://doi.org/10.1099/ijsem.0.002965
    Deptula, Paulina ; Smolander, Olli Pekka ; Laine, Pia ; Roberts, Richard J. ; Edelmann, Minnamari ; Peltola, Petri ; Piironen, Vieno ; Paulin, Lars ; Storgards, Erna ; Savijoki, Kirsi ; Laitila, Arja ; Auvinen, Petri ; Varmanen, Pekka. / Acidipropionibacterium virtanenii sp. nov., isolated from malted barley. In: International Journal of Systematic and Evolutionary Microbiology. 2018 ; Vol. 68, No. 10. pp. 3175-3183.
    @article{c9347bb2d60345dab28eaa8b5887b004,
    title = "Acidipropionibacterium virtanenii sp. nov., isolated from malted barley",
    abstract = "A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 {\%}, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278T to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278T is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol{\%}. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37° C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278T (=VTT E-113202T =DSM 106790T ).",
    keywords = "A, Acidipropionibacterium virtanenii, Acidipropionici, Defence mechanisms, Jensenii, Malted barley, Pseudovitamin b12",
    author = "Paulina Deptula and Smolander, {Olli Pekka} and Pia Laine and Roberts, {Richard J.} and Minnamari Edelmann and Petri Peltola and Vieno Piironen and Lars Paulin and Erna Storgards and Kirsi Savijoki and Arja Laitila and Petri Auvinen and Pekka Varmanen",
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    Deptula, P, Smolander, OP, Laine, P, Roberts, RJ, Edelmann, M, Peltola, P, Piironen, V, Paulin, L, Storgards, E, Savijoki, K, Laitila, A, Auvinen, P & Varmanen, P 2018, 'Acidipropionibacterium virtanenii sp. nov., isolated from malted barley' International Journal of Systematic and Evolutionary Microbiology, vol. 68, no. 10, pp. 3175-3183. https://doi.org/10.1099/ijsem.0.002965

    Acidipropionibacterium virtanenii sp. nov., isolated from malted barley. / Deptula, Paulina (Corresponding Author); Smolander, Olli Pekka; Laine, Pia; Roberts, Richard J.; Edelmann, Minnamari; Peltola, Petri; Piironen, Vieno; Paulin, Lars; Storgards, Erna; Savijoki, Kirsi; Laitila, Arja; Auvinen, Petri; Varmanen, Pekka (Corresponding Author).

    In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 10, 29.08.2018, p. 3175-3183.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Acidipropionibacterium virtanenii sp. nov., isolated from malted barley

    AU - Deptula, Paulina

    AU - Smolander, Olli Pekka

    AU - Laine, Pia

    AU - Roberts, Richard J.

    AU - Edelmann, Minnamari

    AU - Peltola, Petri

    AU - Piironen, Vieno

    AU - Paulin, Lars

    AU - Storgards, Erna

    AU - Savijoki, Kirsi

    AU - Laitila, Arja

    AU - Auvinen, Petri

    AU - Varmanen, Pekka

    PY - 2018/8/29

    Y1 - 2018/8/29

    N2 - A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278T to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278T is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37° C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278T (=VTT E-113202T =DSM 106790T ).

    AB - A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278T to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278T is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37° C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278T (=VTT E-113202T =DSM 106790T ).

    KW - A

    KW - Acidipropionibacterium virtanenii

    KW - Acidipropionici

    KW - Defence mechanisms

    KW - Jensenii

    KW - Malted barley

    KW - Pseudovitamin b12

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    U2 - 10.1099/ijsem.0.002965

    DO - 10.1099/ijsem.0.002965

    M3 - Article

    VL - 68

    SP - 3175

    EP - 3183

    JO - International Journal of Systematic and Evolutionary Microbiology

    T2 - International Journal of Systematic and Evolutionary Microbiology

    JF - International Journal of Systematic and Evolutionary Microbiology

    SN - 1466-5026

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