Actinobacteria and other heterotrophic bacteria in biofilm samples from Roman catacombs

Hanna-Leena Alakomi, Maija-Liisa Suihko, Laura Raaska, Tiina Mattila-Sandholm, Maria Saarela

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

The heterotrophic microbiota (actinobacteria and other heterotrophic bacteria) of two Roman catacombs was characterised. Microbial isolates were identified using phenotypic methods, ribotyping and partial 16S rDNA sequencing. A total of 15 different actinobacterial and 29 other bacterial species were identified. The dominant micro-organisms were filamentous actinobacteria, mainly Streptomyces species (33%). The most commonly occuring other bacteria belonged to the genera Pseudomonas/Stenotrophomonas (28%), Bacillus (17%) and Staphylococcus (14%). In partial 16S rDNA sequencing the similarities to the until now described species indicated that many of these isolates (30%) are potential members of novel species, e.g. within the genera Amycolatopsis and Nocardia, or even within a new genus. Some species may have the ability to deteriorate mineral surfaces, but these properties of the isolates should be studied further.
Original languageEnglish
Title of host publicationProceedings of 10th International Congress Deterioration and Conservation of Stone
EditorsK. Kwiatkowski, R. Löfvendahl
Pages247-252
Volume1
Publication statusPublished - 2004
MoE publication typeA4 Article in a conference publication
Event10th International Congress Deterioration and Conservation of Stone - Stockholm, Sweden
Duration: 27 Jun 20042 Jul 2004

Conference

Conference10th International Congress Deterioration and Conservation of Stone
CountrySweden
CityStockholm
Period27/06/042/07/04

Fingerprint

Actinobacteria
biofilm
Amycolatopsis
Stenotrophomonas
Nocardia
Streptomyces
bacteria
Bacillus (bacteria)
Staphylococcus
DNA fingerprinting
Pseudomonas
new genus
minerals
microorganisms
sampling
methodology
microbiome

Keywords

  • catacomb
  • biofilm
  • microbes
  • identification
  • actinobacteria
  • heterotrops

Cite this

Alakomi, H-L., Suihko, M-L., Raaska, L., Mattila-Sandholm, T., & Saarela, M. (2004). Actinobacteria and other heterotrophic bacteria in biofilm samples from Roman catacombs. In K. Kwiatkowski, & R. Löfvendahl (Eds.), Proceedings of 10th International Congress Deterioration and Conservation of Stone (Vol. 1, pp. 247-252)
Alakomi, Hanna-Leena ; Suihko, Maija-Liisa ; Raaska, Laura ; Mattila-Sandholm, Tiina ; Saarela, Maria. / Actinobacteria and other heterotrophic bacteria in biofilm samples from Roman catacombs. Proceedings of 10th International Congress Deterioration and Conservation of Stone. editor / K. Kwiatkowski ; R. Löfvendahl. Vol. 1 2004. pp. 247-252
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abstract = "The heterotrophic microbiota (actinobacteria and other heterotrophic bacteria) of two Roman catacombs was characterised. Microbial isolates were identified using phenotypic methods, ribotyping and partial 16S rDNA sequencing. A total of 15 different actinobacterial and 29 other bacterial species were identified. The dominant micro-organisms were filamentous actinobacteria, mainly Streptomyces species (33{\%}). The most commonly occuring other bacteria belonged to the genera Pseudomonas/Stenotrophomonas (28{\%}), Bacillus (17{\%}) and Staphylococcus (14{\%}). In partial 16S rDNA sequencing the similarities to the until now described species indicated that many of these isolates (30{\%}) are potential members of novel species, e.g. within the genera Amycolatopsis and Nocardia, or even within a new genus. Some species may have the ability to deteriorate mineral surfaces, but these properties of the isolates should be studied further.",
keywords = "catacomb, biofilm, microbes, identification, actinobacteria, heterotrops",
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Alakomi, H-L, Suihko, M-L, Raaska, L, Mattila-Sandholm, T & Saarela, M 2004, Actinobacteria and other heterotrophic bacteria in biofilm samples from Roman catacombs. in K Kwiatkowski & R Löfvendahl (eds), Proceedings of 10th International Congress Deterioration and Conservation of Stone. vol. 1, pp. 247-252, 10th International Congress Deterioration and Conservation of Stone, Stockholm, Sweden, 27/06/04.

Actinobacteria and other heterotrophic bacteria in biofilm samples from Roman catacombs. / Alakomi, Hanna-Leena; Suihko, Maija-Liisa; Raaska, Laura; Mattila-Sandholm, Tiina; Saarela, Maria.

Proceedings of 10th International Congress Deterioration and Conservation of Stone. ed. / K. Kwiatkowski; R. Löfvendahl. Vol. 1 2004. p. 247-252.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

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T1 - Actinobacteria and other heterotrophic bacteria in biofilm samples from Roman catacombs

AU - Alakomi, Hanna-Leena

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AU - Mattila-Sandholm, Tiina

AU - Saarela, Maria

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AB - The heterotrophic microbiota (actinobacteria and other heterotrophic bacteria) of two Roman catacombs was characterised. Microbial isolates were identified using phenotypic methods, ribotyping and partial 16S rDNA sequencing. A total of 15 different actinobacterial and 29 other bacterial species were identified. The dominant micro-organisms were filamentous actinobacteria, mainly Streptomyces species (33%). The most commonly occuring other bacteria belonged to the genera Pseudomonas/Stenotrophomonas (28%), Bacillus (17%) and Staphylococcus (14%). In partial 16S rDNA sequencing the similarities to the until now described species indicated that many of these isolates (30%) are potential members of novel species, e.g. within the genera Amycolatopsis and Nocardia, or even within a new genus. Some species may have the ability to deteriorate mineral surfaces, but these properties of the isolates should be studied further.

KW - catacomb

KW - biofilm

KW - microbes

KW - identification

KW - actinobacteria

KW - heterotrops

M3 - Conference article in proceedings

SN - 91-631-1458-5

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BT - Proceedings of 10th International Congress Deterioration and Conservation of Stone

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Alakomi H-L, Suihko M-L, Raaska L, Mattila-Sandholm T, Saarela M. Actinobacteria and other heterotrophic bacteria in biofilm samples from Roman catacombs. In Kwiatkowski K, Löfvendahl R, editors, Proceedings of 10th International Congress Deterioration and Conservation of Stone. Vol. 1. 2004. p. 247-252