Action of Trichoderma reesei mannanase on galactoglucomannan in pine kraft pulp

Maija Tenkanen (Corresponding Author), Mari Makkonen, Marjukka Perttula, Liisa Viikari, Anita Teleman

Research output: Contribution to journalArticleScientificpeer-review

92 Citations (Scopus)

Abstract

The di-, tri- and tetrasaccharides formed during Trichoderma reesei endo-β-d-mannanase treatment of pine kraft pulp were studied. The oligosaccharides in the hydrolysate were fractionated using size-exclusion, anion exchange and activated carbon chromatography. The primary sequence of the purified oligomers was determined by two-dimensional NMR techniques. The T. reesei mannanase cleaves the β-1,4-glycosidic linkage of d-mannosyl residues attached either to d-mannose or d-glucose. The d-mannosyl residue may also be substituted by a d-galactosyl group. The main disaccharide produced was mannobiose, but a significant amount of 4-O-β-d-glucopyranosyl-d-mannopyranose (GlcMan) was also produced. After extensive hydrolysis the main trisaccharides produced were 4-O-β-d-mannopyranosyl-[6-O-α-galactopyranosyl]-d-mannopyranose (Gal1Man2) and 4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (Glc2Man). Some mannotriose 4-O-β-d-glucopyranosyl-4-O-β-d-mannopyranosyl1-d-mannopyranose (GlcMan2) and 4-O-β-d-glucopyranosyl-[6-O-α-galactopyranosyl]-d-mannopyranose (Gal1GlcMan) were also detected in the hydrolysate. The structures of two tetrasaccharides were studied. They appeared to be 4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (Glc3Man) and 4-O-gb-d-glucopyranosyl-4-O-β-d-mannopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (GlcManGlcMan). According to the results obtained, the galactoglucomannan in pine contains regions in which two or three glucose units are linked together, which further means that it may contain regions with several successive mannose residues. The galactose side groups were found to be attached only to mannose.

Original languageEnglish
Pages (from-to)191 - 204
Number of pages14
JournalJournal of Biotechnology
Volume57
Issue number1 - 3
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Kraft pulp
Mannose
Glucose
Oligosaccharides
Chromatography
Oligomers
Activated carbon
Hydrolysis
Ion exchange
Negative ions
Nuclear magnetic resonance
Trisaccharides
galactoglucomannan
Disaccharides
Galactose
Anions
Carbon

Cite this

Tenkanen, M., Makkonen, M., Perttula, M., Viikari, L., & Teleman, A. (1997). Action of Trichoderma reesei mannanase on galactoglucomannan in pine kraft pulp. Journal of Biotechnology, 57(1 - 3), 191 - 204. https://doi.org/10.1016/S0168-1656(97)00099-0
Tenkanen, Maija ; Makkonen, Mari ; Perttula, Marjukka ; Viikari, Liisa ; Teleman, Anita. / Action of Trichoderma reesei mannanase on galactoglucomannan in pine kraft pulp. In: Journal of Biotechnology. 1997 ; Vol. 57, No. 1 - 3. pp. 191 - 204.
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title = "Action of Trichoderma reesei mannanase on galactoglucomannan in pine kraft pulp",
abstract = "The di-, tri- and tetrasaccharides formed during Trichoderma reesei endo-β-d-mannanase treatment of pine kraft pulp were studied. The oligosaccharides in the hydrolysate were fractionated using size-exclusion, anion exchange and activated carbon chromatography. The primary sequence of the purified oligomers was determined by two-dimensional NMR techniques. The T. reesei mannanase cleaves the β-1,4-glycosidic linkage of d-mannosyl residues attached either to d-mannose or d-glucose. The d-mannosyl residue may also be substituted by a d-galactosyl group. The main disaccharide produced was mannobiose, but a significant amount of 4-O-β-d-glucopyranosyl-d-mannopyranose (GlcMan) was also produced. After extensive hydrolysis the main trisaccharides produced were 4-O-β-d-mannopyranosyl-[6-O-α-galactopyranosyl]-d-mannopyranose (Gal1Man2) and 4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (Glc2Man). Some mannotriose 4-O-β-d-glucopyranosyl-4-O-β-d-mannopyranosyl1-d-mannopyranose (GlcMan2) and 4-O-β-d-glucopyranosyl-[6-O-α-galactopyranosyl]-d-mannopyranose (Gal1GlcMan) were also detected in the hydrolysate. The structures of two tetrasaccharides were studied. They appeared to be 4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (Glc3Man) and 4-O-gb-d-glucopyranosyl-4-O-β-d-mannopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (GlcManGlcMan). According to the results obtained, the galactoglucomannan in pine contains regions in which two or three glucose units are linked together, which further means that it may contain regions with several successive mannose residues. The galactose side groups were found to be attached only to mannose.",
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Tenkanen, M, Makkonen, M, Perttula, M, Viikari, L & Teleman, A 1997, 'Action of Trichoderma reesei mannanase on galactoglucomannan in pine kraft pulp', Journal of Biotechnology, vol. 57, no. 1 - 3, pp. 191 - 204. https://doi.org/10.1016/S0168-1656(97)00099-0

Action of Trichoderma reesei mannanase on galactoglucomannan in pine kraft pulp. / Tenkanen, Maija (Corresponding Author); Makkonen, Mari; Perttula, Marjukka; Viikari, Liisa; Teleman, Anita.

In: Journal of Biotechnology, Vol. 57, No. 1 - 3, 1997, p. 191 - 204.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Action of Trichoderma reesei mannanase on galactoglucomannan in pine kraft pulp

AU - Tenkanen, Maija

AU - Makkonen, Mari

AU - Perttula, Marjukka

AU - Viikari, Liisa

AU - Teleman, Anita

PY - 1997

Y1 - 1997

N2 - The di-, tri- and tetrasaccharides formed during Trichoderma reesei endo-β-d-mannanase treatment of pine kraft pulp were studied. The oligosaccharides in the hydrolysate were fractionated using size-exclusion, anion exchange and activated carbon chromatography. The primary sequence of the purified oligomers was determined by two-dimensional NMR techniques. The T. reesei mannanase cleaves the β-1,4-glycosidic linkage of d-mannosyl residues attached either to d-mannose or d-glucose. The d-mannosyl residue may also be substituted by a d-galactosyl group. The main disaccharide produced was mannobiose, but a significant amount of 4-O-β-d-glucopyranosyl-d-mannopyranose (GlcMan) was also produced. After extensive hydrolysis the main trisaccharides produced were 4-O-β-d-mannopyranosyl-[6-O-α-galactopyranosyl]-d-mannopyranose (Gal1Man2) and 4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (Glc2Man). Some mannotriose 4-O-β-d-glucopyranosyl-4-O-β-d-mannopyranosyl1-d-mannopyranose (GlcMan2) and 4-O-β-d-glucopyranosyl-[6-O-α-galactopyranosyl]-d-mannopyranose (Gal1GlcMan) were also detected in the hydrolysate. The structures of two tetrasaccharides were studied. They appeared to be 4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (Glc3Man) and 4-O-gb-d-glucopyranosyl-4-O-β-d-mannopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (GlcManGlcMan). According to the results obtained, the galactoglucomannan in pine contains regions in which two or three glucose units are linked together, which further means that it may contain regions with several successive mannose residues. The galactose side groups were found to be attached only to mannose.

AB - The di-, tri- and tetrasaccharides formed during Trichoderma reesei endo-β-d-mannanase treatment of pine kraft pulp were studied. The oligosaccharides in the hydrolysate were fractionated using size-exclusion, anion exchange and activated carbon chromatography. The primary sequence of the purified oligomers was determined by two-dimensional NMR techniques. The T. reesei mannanase cleaves the β-1,4-glycosidic linkage of d-mannosyl residues attached either to d-mannose or d-glucose. The d-mannosyl residue may also be substituted by a d-galactosyl group. The main disaccharide produced was mannobiose, but a significant amount of 4-O-β-d-glucopyranosyl-d-mannopyranose (GlcMan) was also produced. After extensive hydrolysis the main trisaccharides produced were 4-O-β-d-mannopyranosyl-[6-O-α-galactopyranosyl]-d-mannopyranose (Gal1Man2) and 4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (Glc2Man). Some mannotriose 4-O-β-d-glucopyranosyl-4-O-β-d-mannopyranosyl1-d-mannopyranose (GlcMan2) and 4-O-β-d-glucopyranosyl-[6-O-α-galactopyranosyl]-d-mannopyranose (Gal1GlcMan) were also detected in the hydrolysate. The structures of two tetrasaccharides were studied. They appeared to be 4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (Glc3Man) and 4-O-gb-d-glucopyranosyl-4-O-β-d-mannopyranosyl-4-O-β-d-glucopyranosyl-d-mannopyranose (GlcManGlcMan). According to the results obtained, the galactoglucomannan in pine contains regions in which two or three glucose units are linked together, which further means that it may contain regions with several successive mannose residues. The galactose side groups were found to be attached only to mannose.

U2 - 10.1016/S0168-1656(97)00099-0

DO - 10.1016/S0168-1656(97)00099-0

M3 - Article

VL - 57

SP - 191

EP - 204

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 1 - 3

ER -