Activity of an Aspergillus terreus α-arabinofuranosidase on phenolic-substituted oligosaccharides

Elina Luonteri (Corresponding Author), Paul Kroon, Maija Tenkanen, Anita Teleman, Gary Williamson

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

The effect of phenolic substitutions on the activity of an α-arabinofuranosidase from Aspergillus terreus was investigated using feruloylated oligosaccharides isolated from plant cell walls, equivalent oligosaccharides obtained through treatment with specific ferulic acid esterases, and a synthetic lignin-carbohydrate complex (LCC). Feruloyl substituents limited the hydrolysis of arabinoxylan and arabinan oligosaccharides but only if the feruloyl group was esterified to the terminal non-reducing arabinose. Somewhat surprisingly, the LCC-model compound, in which the arabinose residue is substituted with a bulky dilignol group, was degraded by the enzyme. This indicated that the enzyme is able to approach this linkage from the xylose side.
Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalJournal of Biotechnology
Volume67
Issue number1
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

Fingerprint

Oligosaccharides
Aspergillus
Arabinose
Lignin
Carbohydrates
Enzymes
Xylose
Plant Cells
Cell Wall
Hydrolysis
Substitution reactions
Acids

Cite this

Luonteri, Elina ; Kroon, Paul ; Tenkanen, Maija ; Teleman, Anita ; Williamson, Gary. / Activity of an Aspergillus terreus α-arabinofuranosidase on phenolic-substituted oligosaccharides. In: Journal of Biotechnology. 1999 ; Vol. 67, No. 1. pp. 41-48.
@article{34982a3f96a14b34a4f025bda721d273,
title = "Activity of an Aspergillus terreus α-arabinofuranosidase on phenolic-substituted oligosaccharides",
abstract = "The effect of phenolic substitutions on the activity of an α-arabinofuranosidase from Aspergillus terreus was investigated using feruloylated oligosaccharides isolated from plant cell walls, equivalent oligosaccharides obtained through treatment with specific ferulic acid esterases, and a synthetic lignin-carbohydrate complex (LCC). Feruloyl substituents limited the hydrolysis of arabinoxylan and arabinan oligosaccharides but only if the feruloyl group was esterified to the terminal non-reducing arabinose. Somewhat surprisingly, the LCC-model compound, in which the arabinose residue is substituted with a bulky dilignol group, was degraded by the enzyme. This indicated that the enzyme is able to approach this linkage from the xylose side.",
author = "Elina Luonteri and Paul Kroon and Maija Tenkanen and Anita Teleman and Gary Williamson",
year = "1999",
doi = "10.1016/S0168-1656(98)00164-3",
language = "English",
volume = "67",
pages = "41--48",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "1",

}

Activity of an Aspergillus terreus α-arabinofuranosidase on phenolic-substituted oligosaccharides. / Luonteri, Elina (Corresponding Author); Kroon, Paul; Tenkanen, Maija; Teleman, Anita; Williamson, Gary.

In: Journal of Biotechnology, Vol. 67, No. 1, 1999, p. 41-48.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Activity of an Aspergillus terreus α-arabinofuranosidase on phenolic-substituted oligosaccharides

AU - Luonteri, Elina

AU - Kroon, Paul

AU - Tenkanen, Maija

AU - Teleman, Anita

AU - Williamson, Gary

PY - 1999

Y1 - 1999

N2 - The effect of phenolic substitutions on the activity of an α-arabinofuranosidase from Aspergillus terreus was investigated using feruloylated oligosaccharides isolated from plant cell walls, equivalent oligosaccharides obtained through treatment with specific ferulic acid esterases, and a synthetic lignin-carbohydrate complex (LCC). Feruloyl substituents limited the hydrolysis of arabinoxylan and arabinan oligosaccharides but only if the feruloyl group was esterified to the terminal non-reducing arabinose. Somewhat surprisingly, the LCC-model compound, in which the arabinose residue is substituted with a bulky dilignol group, was degraded by the enzyme. This indicated that the enzyme is able to approach this linkage from the xylose side.

AB - The effect of phenolic substitutions on the activity of an α-arabinofuranosidase from Aspergillus terreus was investigated using feruloylated oligosaccharides isolated from plant cell walls, equivalent oligosaccharides obtained through treatment with specific ferulic acid esterases, and a synthetic lignin-carbohydrate complex (LCC). Feruloyl substituents limited the hydrolysis of arabinoxylan and arabinan oligosaccharides but only if the feruloyl group was esterified to the terminal non-reducing arabinose. Somewhat surprisingly, the LCC-model compound, in which the arabinose residue is substituted with a bulky dilignol group, was degraded by the enzyme. This indicated that the enzyme is able to approach this linkage from the xylose side.

U2 - 10.1016/S0168-1656(98)00164-3

DO - 10.1016/S0168-1656(98)00164-3

M3 - Article

VL - 67

SP - 41

EP - 48

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 1

ER -