Activity of laccase on unbleached and bleached thermomechanical pulp

Stina Grönqvist; Johanna Buchert; K. Rantanen; Liisa Viikari; Anna Suurnäkki

doi:10.1016/S0141-0229(02)00319-8

Activity of laccase on unbleached and bleached thermomechanical pulp

Stina Grönqvist (Corresponding Author), Johanna Buchert, K. Rantanen, Liisa Viikari, Anna Suurnäkki

University of Jyväskylä

Research output: Contribution to journal › Article › Scientific › peer-review

49 Citations (Scopus)

Abstract

The introduction of value-added properties to pulp fibres is an attractive proposition. One interesting option is targeted modification of fibre surfaces by enzymatic activation. In this work, the activity of laccase on pulp and pulp fractions from thermomechanical pulp (TMP) and peroxide bleached TMP was studied on the basis of consumption of the co-substrate oxygen in the reaction and by studying the formation of radicals in the pulp material as analysed by electron paramagnetic resonance spectroscopy (EPR). Laccases obtained from Trametes hirsuta and Myceliophthora thermophila were used in the study. Laccases were found to be active on pulp material of unbleached TMP, whereas only fines from bleached TMP reacted in laccase treatment. Dissolved and colloidal substances (DCS) were assumed to have a mediating role in the laccase-catalysed oxidation of the fibre-bound material.

Original language	English
Pages (from-to)	439-445
Number of pages	7
Journal	Enzyme and Microbial Technology
Volume	32
Issue number	3-4
DOIs	https://doi.org/10.1016/S0141-0229(02)00319-8
Publication status	Published - 2003
MoE publication type	A1 Journal article-refereed

Keywords

Activation of fibres
Activity of laccase on pulp
Laccase

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10.1016/S0141-0229(02)00319-8

Cite this

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title = "Activity of laccase on unbleached and bleached thermomechanical pulp",

abstract = "The introduction of value-added properties to pulp fibres is an attractive proposition. One interesting option is targeted modification of fibre surfaces by enzymatic activation. In this work, the activity of laccase on pulp and pulp fractions from thermomechanical pulp (TMP) and peroxide bleached TMP was studied on the basis of consumption of the co-substrate oxygen in the reaction and by studying the formation of radicals in the pulp material as analysed by electron paramagnetic resonance spectroscopy (EPR). Laccases obtained from Trametes hirsuta and Myceliophthora thermophila were used in the study. Laccases were found to be active on pulp material of unbleached TMP, whereas only fines from bleached TMP reacted in laccase treatment. Dissolved and colloidal substances (DCS) were assumed to have a mediating role in the laccase-catalysed oxidation of the fibre-bound material.",

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year = "2003",

doi = "10.1016/S0141-0229(02)00319-8",

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volume = "32",

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journal = "Enzyme and Microbial Technology",

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T1 - Activity of laccase on unbleached and bleached thermomechanical pulp

AU - Grönqvist, Stina

AU - Buchert, Johanna

AU - Rantanen, K.

AU - Viikari, Liisa

AU - Suurnäkki, Anna

PY - 2003

Y1 - 2003

N2 - The introduction of value-added properties to pulp fibres is an attractive proposition. One interesting option is targeted modification of fibre surfaces by enzymatic activation. In this work, the activity of laccase on pulp and pulp fractions from thermomechanical pulp (TMP) and peroxide bleached TMP was studied on the basis of consumption of the co-substrate oxygen in the reaction and by studying the formation of radicals in the pulp material as analysed by electron paramagnetic resonance spectroscopy (EPR). Laccases obtained from Trametes hirsuta and Myceliophthora thermophila were used in the study. Laccases were found to be active on pulp material of unbleached TMP, whereas only fines from bleached TMP reacted in laccase treatment. Dissolved and colloidal substances (DCS) were assumed to have a mediating role in the laccase-catalysed oxidation of the fibre-bound material.

AB - The introduction of value-added properties to pulp fibres is an attractive proposition. One interesting option is targeted modification of fibre surfaces by enzymatic activation. In this work, the activity of laccase on pulp and pulp fractions from thermomechanical pulp (TMP) and peroxide bleached TMP was studied on the basis of consumption of the co-substrate oxygen in the reaction and by studying the formation of radicals in the pulp material as analysed by electron paramagnetic resonance spectroscopy (EPR). Laccases obtained from Trametes hirsuta and Myceliophthora thermophila were used in the study. Laccases were found to be active on pulp material of unbleached TMP, whereas only fines from bleached TMP reacted in laccase treatment. Dissolved and colloidal substances (DCS) were assumed to have a mediating role in the laccase-catalysed oxidation of the fibre-bound material.

KW - Activation of fibres

KW - Activity of laccase on pulp

KW - Laccase

U2 - 10.1016/S0141-0229(02)00319-8

DO - 10.1016/S0141-0229(02)00319-8

M3 - Article

SN - 0141-0229

VL - 32

SP - 439

EP - 445

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

IS - 3-4

ER -

Activity of laccase on unbleached and bleached thermomechanical pulp

Abstract

Keywords

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