Activity of laccase on unbleached and bleached thermomechanical pulp

Stina Grönqvist (Corresponding Author), Johanna Buchert, K. Rantanen, Liisa Viikari, Anna Suurnäkki

Research output: Contribution to journalArticleScientificpeer-review

48 Citations (Scopus)

Abstract

The introduction of value-added properties to pulp fibres is an attractive proposition. One interesting option is targeted modification of fibre surfaces by enzymatic activation. In this work, the activity of laccase on pulp and pulp fractions from thermomechanical pulp (TMP) and peroxide bleached TMP was studied on the basis of consumption of the co-substrate oxygen in the reaction and by studying the formation of radicals in the pulp material as analysed by electron paramagnetic resonance spectroscopy (EPR). Laccases obtained from Trametes hirsuta and Myceliophthora thermophila were used in the study. Laccases were found to be active on pulp material of unbleached TMP, whereas only fines from bleached TMP reacted in laccase treatment. Dissolved and colloidal substances (DCS) were assumed to have a mediating role in the laccase-catalysed oxidation of the fibre-bound material.
Original languageEnglish
Pages (from-to)439-445
Number of pages7
JournalEnzyme and Microbial Technology
Volume32
Issue number3-4
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

Thermomechanical pulp
Laccase
Bleached pulp
Pulp materials
Pulp
Fibers
Unbleached pulp
Trametes
Peroxides
Paramagnetic resonance
Electron Spin Resonance Spectroscopy
Chemical activation
Spectroscopy
Spectrum Analysis
Oxidation
Oxygen
Substrates

Keywords

  • Activation of fibres
  • Activity of laccase on pulp
  • Laccase

Cite this

Grönqvist, Stina ; Buchert, Johanna ; Rantanen, K. ; Viikari, Liisa ; Suurnäkki, Anna. / Activity of laccase on unbleached and bleached thermomechanical pulp. In: Enzyme and Microbial Technology. 2003 ; Vol. 32, No. 3-4. pp. 439-445.
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Activity of laccase on unbleached and bleached thermomechanical pulp. / Grönqvist, Stina (Corresponding Author); Buchert, Johanna; Rantanen, K.; Viikari, Liisa; Suurnäkki, Anna.

In: Enzyme and Microbial Technology, Vol. 32, No. 3-4, 2003, p. 439-445.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Activity of laccase on unbleached and bleached thermomechanical pulp

AU - Grönqvist, Stina

AU - Buchert, Johanna

AU - Rantanen, K.

AU - Viikari, Liisa

AU - Suurnäkki, Anna

PY - 2003

Y1 - 2003

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AB - The introduction of value-added properties to pulp fibres is an attractive proposition. One interesting option is targeted modification of fibre surfaces by enzymatic activation. In this work, the activity of laccase on pulp and pulp fractions from thermomechanical pulp (TMP) and peroxide bleached TMP was studied on the basis of consumption of the co-substrate oxygen in the reaction and by studying the formation of radicals in the pulp material as analysed by electron paramagnetic resonance spectroscopy (EPR). Laccases obtained from Trametes hirsuta and Myceliophthora thermophila were used in the study. Laccases were found to be active on pulp material of unbleached TMP, whereas only fines from bleached TMP reacted in laccase treatment. Dissolved and colloidal substances (DCS) were assumed to have a mediating role in the laccase-catalysed oxidation of the fibre-bound material.

KW - Activation of fibres

KW - Activity of laccase on pulp

KW - Laccase

U2 - 10.1016/S0141-0229(02)00319-8

DO - 10.1016/S0141-0229(02)00319-8

M3 - Article

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SP - 439

EP - 445

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 3-4

ER -