Abstract
Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.
| Original language | English |
|---|---|
| Pages (from-to) | 57-63 |
| Number of pages | 7 |
| Journal | FEMS Microbiology Letters |
| Volume | 198 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 20 Apr 2001 |
| MoE publication type | A1 Journal article-refereed |
Fingerprint
Keywords
- Cellulose-binding domain
- Chitin-binding domain
- Chitinase
- Enzyme engineering
- Trichoderma harzianum
Cite this
}
Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum. / Limón, M. Carmen; Margolles-Clark, Emilio; Benítez, Tahía; Penttilä, Merja.
In: FEMS Microbiology Letters, Vol. 198, No. 1, 20.04.2001, p. 57-63.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum
AU - Limón, M. Carmen
AU - Margolles-Clark, Emilio
AU - Benítez, Tahía
AU - Penttilä, Merja
PY - 2001/4/20
Y1 - 2001/4/20
N2 - Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.
AB - Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.
KW - Cellulose-binding domain
KW - Chitin-binding domain
KW - Chitinase
KW - Enzyme engineering
KW - Trichoderma harzianum
UR - http://www.scopus.com/inward/record.url?scp=0035917427&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2001.tb10619.x
DO - 10.1111/j.1574-6968.2001.tb10619.x
M3 - Article
VL - 198
SP - 57
EP - 63
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -