Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum

M. Carmen Limón, Emilio Margolles-Clark, Tahía Benítez, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

58 Citations (Scopus)

Abstract

Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalFEMS Microbiology Letters
Volume198
Issue number1
DOIs
Publication statusPublished - 20 Apr 2001
MoE publication typeA1 Journal article-refereed

Fingerprint

Chitinases
Trichoderma
Chitin
Cellulose 1,4-beta-Cellobiosidase
Cellulose
Cell Wall
Tobacco
Fungi

Keywords

  • Cellulose-binding domain
  • Chitin-binding domain
  • Chitinase
  • Enzyme engineering
  • Trichoderma harzianum

Cite this

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title = "Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum",
abstract = "Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.",
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journal = "FEMS Microbiology Letters",
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Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum. / Limón, M. Carmen; Margolles-Clark, Emilio; Benítez, Tahía; Penttilä, Merja.

In: FEMS Microbiology Letters, Vol. 198, No. 1, 20.04.2001, p. 57-63.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum

AU - Limón, M. Carmen

AU - Margolles-Clark, Emilio

AU - Benítez, Tahía

AU - Penttilä, Merja

PY - 2001/4/20

Y1 - 2001/4/20

N2 - Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.

AB - Chitinase Chit42 from Trichoderma harzianum CECT 2413 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin-binding domain (ChBD). We have produced hybrid chitinases with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Nicotiana tabacum ChiA chitinase and the cellulose-binding domain from cellobiohydrolase II of Trichoderma reesei. The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.

KW - Cellulose-binding domain

KW - Chitin-binding domain

KW - Chitinase

KW - Enzyme engineering

KW - Trichoderma harzianum

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U2 - 10.1111/j.1574-6968.2001.tb10619.x

DO - 10.1111/j.1574-6968.2001.tb10619.x

M3 - Article

VL - 198

SP - 57

EP - 63

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

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ER -