Adhesion and tribological properties of hydrophobin proteins in aqueous lubrication on stainless steel surfaces

Timo J. Hakala (Corresponding Author), Päivi Laaksonen, Vesa Saikko, Tiina Ahlroos, Aino Helle, Riitta Mahlberg, Hendrik Hähl, Karin Jacobs, Petri Kuosmanen, Markus, B. Linder, Kenneth Holmberg

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

Macroscale tribological properties of hydrophobin layers bound on stainless steel surfaces were investigated in an aqueous environment. Emphasis was on boundary lubrication because water easily fails in hydrodynamic lubrication due to its low viscosity. We studied the affinities of two different proteins, HFBI and FpHYD5, on stainless steel and their ability to bind water at the surface by combining quartz crystal microbalance (QCM-D) and ellipsometry. Both proteins contained an adhesive hydrophobic domain, but FpHYD5 also had a very strongly hydrating carbohydrate structure attached to it. The lubrication properties of the proteins were studied with two different methods, pin-on-disc (POD) (stainless steel vs. stainless steel) and circular translation pin-on-disc (CTPOD) (UHMWPE vs. stainless steel). It was observed that both hydrophobins could adhere to the stainless steel surface and form highly hydrated layers. Both proteins reduced friction and wear of the sliding contact between two stainless steel surfaces. With UHMWPE against stainless steel, the hydrophobins prevented the polyethylene transfer to the counterface. The lowest coefficient of friction (COF) 0.13 was observed when FpHYD5 hydrophobins were employed in pure water. On the other hand, the lowest wear was observed when FpHYD5 proteins were added in a 50 mM sodium acetate buffer. Increasing the water content and loosening the hydrophobin film structure on the stainless steel surface led to a reduction in friction and wear.
Original languageEnglish
Pages (from-to)9867-9872
Number of pages5
JournalRSC Advances
Volume2
Issue number26
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Stainless Steel
Lubrication
Adhesion
Stainless steel
Proteins
Ultrahigh molecular weight polyethylenes
Wear of materials
Friction
Water
Sodium Acetate
Quartz crystal microbalances
Ellipsometry
Polyethylene
Carbohydrates
Water content
Polyethylenes
Adhesives
Buffers
Hydrodynamics
Sodium

Keywords

  • Water lubrication
  • hydrophobins
  • friction

Cite this

Hakala, Timo J. ; Laaksonen, Päivi ; Saikko, Vesa ; Ahlroos, Tiina ; Helle, Aino ; Mahlberg, Riitta ; Hähl, Hendrik ; Jacobs, Karin ; Kuosmanen, Petri ; Linder, Markus, B. ; Holmberg, Kenneth. / Adhesion and tribological properties of hydrophobin proteins in aqueous lubrication on stainless steel surfaces. In: RSC Advances. 2012 ; Vol. 2, No. 26. pp. 9867-9872.
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abstract = "Macroscale tribological properties of hydrophobin layers bound on stainless steel surfaces were investigated in an aqueous environment. Emphasis was on boundary lubrication because water easily fails in hydrodynamic lubrication due to its low viscosity. We studied the affinities of two different proteins, HFBI and FpHYD5, on stainless steel and their ability to bind water at the surface by combining quartz crystal microbalance (QCM-D) and ellipsometry. Both proteins contained an adhesive hydrophobic domain, but FpHYD5 also had a very strongly hydrating carbohydrate structure attached to it. The lubrication properties of the proteins were studied with two different methods, pin-on-disc (POD) (stainless steel vs. stainless steel) and circular translation pin-on-disc (CTPOD) (UHMWPE vs. stainless steel). It was observed that both hydrophobins could adhere to the stainless steel surface and form highly hydrated layers. Both proteins reduced friction and wear of the sliding contact between two stainless steel surfaces. With UHMWPE against stainless steel, the hydrophobins prevented the polyethylene transfer to the counterface. The lowest coefficient of friction (COF) 0.13 was observed when FpHYD5 hydrophobins were employed in pure water. On the other hand, the lowest wear was observed when FpHYD5 proteins were added in a 50 mM sodium acetate buffer. Increasing the water content and loosening the hydrophobin film structure on the stainless steel surface led to a reduction in friction and wear.",
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author = "Hakala, {Timo J.} and P{\"a}ivi Laaksonen and Vesa Saikko and Tiina Ahlroos and Aino Helle and Riitta Mahlberg and Hendrik H{\"a}hl and Karin Jacobs and Petri Kuosmanen and Linder, {Markus, B.} and Kenneth Holmberg",
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Hakala, TJ, Laaksonen, P, Saikko, V, Ahlroos, T, Helle, A, Mahlberg, R, Hähl, H, Jacobs, K, Kuosmanen, P, Linder, MB & Holmberg, K 2012, 'Adhesion and tribological properties of hydrophobin proteins in aqueous lubrication on stainless steel surfaces', RSC Advances, vol. 2, no. 26, pp. 9867-9872. https://doi.org/10.1039/C2RA21018E

Adhesion and tribological properties of hydrophobin proteins in aqueous lubrication on stainless steel surfaces. / Hakala, Timo J. (Corresponding Author); Laaksonen, Päivi; Saikko, Vesa; Ahlroos, Tiina; Helle, Aino; Mahlberg, Riitta; Hähl, Hendrik; Jacobs, Karin; Kuosmanen, Petri; Linder, Markus, B.; Holmberg, Kenneth.

In: RSC Advances, Vol. 2, No. 26, 2012, p. 9867-9872.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Adhesion and tribological properties of hydrophobin proteins in aqueous lubrication on stainless steel surfaces

AU - Hakala, Timo J.

AU - Laaksonen, Päivi

AU - Saikko, Vesa

AU - Ahlroos, Tiina

AU - Helle, Aino

AU - Mahlberg, Riitta

AU - Hähl, Hendrik

AU - Jacobs, Karin

AU - Kuosmanen, Petri

AU - Linder, Markus, B.

AU - Holmberg, Kenneth

N1 - Project code: 34126 Project code: 41202

PY - 2012

Y1 - 2012

N2 - Macroscale tribological properties of hydrophobin layers bound on stainless steel surfaces were investigated in an aqueous environment. Emphasis was on boundary lubrication because water easily fails in hydrodynamic lubrication due to its low viscosity. We studied the affinities of two different proteins, HFBI and FpHYD5, on stainless steel and their ability to bind water at the surface by combining quartz crystal microbalance (QCM-D) and ellipsometry. Both proteins contained an adhesive hydrophobic domain, but FpHYD5 also had a very strongly hydrating carbohydrate structure attached to it. The lubrication properties of the proteins were studied with two different methods, pin-on-disc (POD) (stainless steel vs. stainless steel) and circular translation pin-on-disc (CTPOD) (UHMWPE vs. stainless steel). It was observed that both hydrophobins could adhere to the stainless steel surface and form highly hydrated layers. Both proteins reduced friction and wear of the sliding contact between two stainless steel surfaces. With UHMWPE against stainless steel, the hydrophobins prevented the polyethylene transfer to the counterface. The lowest coefficient of friction (COF) 0.13 was observed when FpHYD5 hydrophobins were employed in pure water. On the other hand, the lowest wear was observed when FpHYD5 proteins were added in a 50 mM sodium acetate buffer. Increasing the water content and loosening the hydrophobin film structure on the stainless steel surface led to a reduction in friction and wear.

AB - Macroscale tribological properties of hydrophobin layers bound on stainless steel surfaces were investigated in an aqueous environment. Emphasis was on boundary lubrication because water easily fails in hydrodynamic lubrication due to its low viscosity. We studied the affinities of two different proteins, HFBI and FpHYD5, on stainless steel and their ability to bind water at the surface by combining quartz crystal microbalance (QCM-D) and ellipsometry. Both proteins contained an adhesive hydrophobic domain, but FpHYD5 also had a very strongly hydrating carbohydrate structure attached to it. The lubrication properties of the proteins were studied with two different methods, pin-on-disc (POD) (stainless steel vs. stainless steel) and circular translation pin-on-disc (CTPOD) (UHMWPE vs. stainless steel). It was observed that both hydrophobins could adhere to the stainless steel surface and form highly hydrated layers. Both proteins reduced friction and wear of the sliding contact between two stainless steel surfaces. With UHMWPE against stainless steel, the hydrophobins prevented the polyethylene transfer to the counterface. The lowest coefficient of friction (COF) 0.13 was observed when FpHYD5 hydrophobins were employed in pure water. On the other hand, the lowest wear was observed when FpHYD5 proteins were added in a 50 mM sodium acetate buffer. Increasing the water content and loosening the hydrophobin film structure on the stainless steel surface led to a reduction in friction and wear.

KW - Water lubrication

KW - hydrophobins

KW - friction

U2 - 10.1039/C2RA21018E

DO - 10.1039/C2RA21018E

M3 - Article

VL - 2

SP - 9867

EP - 9872

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 26

ER -