Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies

Ross Crittenden (Corresponding Author), Arja Laitila, Pirkko Forssell, Jaana Mättö, Maria Saarela, Tiina Mattila-Sandholm, Päivi Myllärinen

Research output: Contribution to journalArticleScientificpeer-review

136 Citations (Scopus)

Abstract

Adhesion of 19 Bifidobacterium strains to native maize, potato, oat, and barley starch granules was examined to investigate links between adhesion and substrate utilization and to determine if adhesion to starch could be exploited in probiotic food technologies. Starch adhesion was not characteristic of all the bifidobacteria tested. Adherent bacteria bound similarly to the different types of starch, and the binding capacity of the starch (number of bacteria per gram) correlated to the surface area of the granules. Highly adherent strains were able to hydrolyze the granular starches, but not all amylolytic strains were adherent, indicating that starch adhesion is not a prerequisite for efficient substrate utilization for all bifidobacteria. Adhesion was mediated by a cell surface protein(s). For the model organisms tested (Bifidobacterium adolescentis VTT E-001561 and Bifidobacterium pseudolongum ATCC 25526), adhesion appeared to be specific for α-1,4-linked glucose sugars, since adhesion was inhibited by maltose, maltodextrin, amylose, and soluble starch but not by trehalose, cellobiose, or lactose. In an in vitro gastric model, adhesion was inhibited both by the action of protease and at pH values of ≤3. Adhesion was not affected by bile, but the binding capacity of the starch was reduced by exposure to pancreatin. It may be possible to exploit adhesion of probiotic bifidobacteria to starch granules in microencapsulation technology and for synbiotic food applications.
Original languageEnglish
Pages (from-to)3469-3475
Number of pages7
JournalApplied and Environmental Microbiology
Volume67
Issue number8
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

Fingerprint

Bifidobacterium
probiotics
Probiotics
adhesion
starch
Starch
Technology
Food Technology
binding capacity
starch granules
Bifidobacterium pseudolongum
Synbiotics
oat starch
Pancreatin
Bifidobacterium adolescentis
Bacteria
barley starch
Cellobiose
Drug Compounding
Amylose

Cite this

Crittenden, R., Laitila, A., Forssell, P., Mättö, J., Saarela, M., Mattila-Sandholm, T., & Myllärinen, P. (2001). Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies. Applied and Environmental Microbiology, 67(8), 3469-3475. https://doi.org/10.1128/AEM.67.8.3469-3475.2001
Crittenden, Ross ; Laitila, Arja ; Forssell, Pirkko ; Mättö, Jaana ; Saarela, Maria ; Mattila-Sandholm, Tiina ; Myllärinen, Päivi. / Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies. In: Applied and Environmental Microbiology. 2001 ; Vol. 67, No. 8. pp. 3469-3475.
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abstract = "Adhesion of 19 Bifidobacterium strains to native maize, potato, oat, and barley starch granules was examined to investigate links between adhesion and substrate utilization and to determine if adhesion to starch could be exploited in probiotic food technologies. Starch adhesion was not characteristic of all the bifidobacteria tested. Adherent bacteria bound similarly to the different types of starch, and the binding capacity of the starch (number of bacteria per gram) correlated to the surface area of the granules. Highly adherent strains were able to hydrolyze the granular starches, but not all amylolytic strains were adherent, indicating that starch adhesion is not a prerequisite for efficient substrate utilization for all bifidobacteria. Adhesion was mediated by a cell surface protein(s). For the model organisms tested (Bifidobacterium adolescentis VTT E-001561 and Bifidobacterium pseudolongum ATCC 25526), adhesion appeared to be specific for α-1,4-linked glucose sugars, since adhesion was inhibited by maltose, maltodextrin, amylose, and soluble starch but not by trehalose, cellobiose, or lactose. In an in vitro gastric model, adhesion was inhibited both by the action of protease and at pH values of ≤3. Adhesion was not affected by bile, but the binding capacity of the starch was reduced by exposure to pancreatin. It may be possible to exploit adhesion of probiotic bifidobacteria to starch granules in microencapsulation technology and for synbiotic food applications.",
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Crittenden, R, Laitila, A, Forssell, P, Mättö, J, Saarela, M, Mattila-Sandholm, T & Myllärinen, P 2001, 'Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies', Applied and Environmental Microbiology, vol. 67, no. 8, pp. 3469-3475. https://doi.org/10.1128/AEM.67.8.3469-3475.2001

Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies. / Crittenden, Ross (Corresponding Author); Laitila, Arja; Forssell, Pirkko; Mättö, Jaana; Saarela, Maria; Mattila-Sandholm, Tiina; Myllärinen, Päivi.

In: Applied and Environmental Microbiology, Vol. 67, No. 8, 2001, p. 3469-3475.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies

AU - Crittenden, Ross

AU - Laitila, Arja

AU - Forssell, Pirkko

AU - Mättö, Jaana

AU - Saarela, Maria

AU - Mattila-Sandholm, Tiina

AU - Myllärinen, Päivi

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N2 - Adhesion of 19 Bifidobacterium strains to native maize, potato, oat, and barley starch granules was examined to investigate links between adhesion and substrate utilization and to determine if adhesion to starch could be exploited in probiotic food technologies. Starch adhesion was not characteristic of all the bifidobacteria tested. Adherent bacteria bound similarly to the different types of starch, and the binding capacity of the starch (number of bacteria per gram) correlated to the surface area of the granules. Highly adherent strains were able to hydrolyze the granular starches, but not all amylolytic strains were adherent, indicating that starch adhesion is not a prerequisite for efficient substrate utilization for all bifidobacteria. Adhesion was mediated by a cell surface protein(s). For the model organisms tested (Bifidobacterium adolescentis VTT E-001561 and Bifidobacterium pseudolongum ATCC 25526), adhesion appeared to be specific for α-1,4-linked glucose sugars, since adhesion was inhibited by maltose, maltodextrin, amylose, and soluble starch but not by trehalose, cellobiose, or lactose. In an in vitro gastric model, adhesion was inhibited both by the action of protease and at pH values of ≤3. Adhesion was not affected by bile, but the binding capacity of the starch was reduced by exposure to pancreatin. It may be possible to exploit adhesion of probiotic bifidobacteria to starch granules in microencapsulation technology and for synbiotic food applications.

AB - Adhesion of 19 Bifidobacterium strains to native maize, potato, oat, and barley starch granules was examined to investigate links between adhesion and substrate utilization and to determine if adhesion to starch could be exploited in probiotic food technologies. Starch adhesion was not characteristic of all the bifidobacteria tested. Adherent bacteria bound similarly to the different types of starch, and the binding capacity of the starch (number of bacteria per gram) correlated to the surface area of the granules. Highly adherent strains were able to hydrolyze the granular starches, but not all amylolytic strains were adherent, indicating that starch adhesion is not a prerequisite for efficient substrate utilization for all bifidobacteria. Adhesion was mediated by a cell surface protein(s). For the model organisms tested (Bifidobacterium adolescentis VTT E-001561 and Bifidobacterium pseudolongum ATCC 25526), adhesion appeared to be specific for α-1,4-linked glucose sugars, since adhesion was inhibited by maltose, maltodextrin, amylose, and soluble starch but not by trehalose, cellobiose, or lactose. In an in vitro gastric model, adhesion was inhibited both by the action of protease and at pH values of ≤3. Adhesion was not affected by bile, but the binding capacity of the starch was reduced by exposure to pancreatin. It may be possible to exploit adhesion of probiotic bifidobacteria to starch granules in microencapsulation technology and for synbiotic food applications.

U2 - 10.1128/AEM.67.8.3469-3475.2001

DO - 10.1128/AEM.67.8.3469-3475.2001

M3 - Article

VL - 67

SP - 3469

EP - 3475

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 8

ER -