Adhesion Properties of Freestanding Hydrophobin Bilayers

Hendrik Hähl (Corresponding Author), Jose Nabor Vargas, Michael Jung, Alessandra Griffo, Päivi Laaksonen, Michael Lienemann, Karin Jacobs, Ralf Seemann, Jean Baptiste Fleury (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Hydrophobins are a family of small-sized proteins featuring a distinct hydrophobic patch on the protein's surface, rendering them amphiphilic. This particularity allows hydrophobins to self-assemble into monolayers at any hydrophilic/hydrophobic interface. Moreover, stable pure protein bilayers can be created from two interfacial hydrophobin monolayers by contacting either their hydrophobic or their hydrophilic sides. In this study, this is achieved via a microfluidic approach, in which also the bilayers' adhesion energy can be determined. This enables us to study the origin of the adhesion of hydrophobic and hydrophilic core bilayers made from the class II hydrophobins HFBI and HFBII. Using different fluid media in this setup and introducing genetically modified variants of the HFBI molecule, the different force contributions to the adhesion of the bilayer sheets are studied. It was found that in the hydrophilic contact situation, the adhesive interaction was higher than that in the hydrophobic contact situation and could be even enhanced by reducing the contributions of electrostatic interactions. This effect indicates that the van der Waals interaction is the dominant contribution that explains the stability of the observed bilayers.

Original languageEnglish
Pages (from-to)8542-8549
Number of pages8
JournalLangmuir
Volume34
Issue number29
DOIs
Publication statusPublished - 24 Jul 2018
MoE publication typeA1 Journal article-refereed

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adhesion
Adhesion
proteins
Proteins
Monolayers
interactions
Coulomb interactions
Microfluidics
adhesives
Adhesives
Membrane Proteins
electrostatics
Molecules
Fluids
fluids
molecules
1-(heptafluorobutyryl)imidazole
energy

Cite this

Hähl, H., Vargas, J. N., Jung, M., Griffo, A., Laaksonen, P., Lienemann, M., ... Fleury, J. B. (2018). Adhesion Properties of Freestanding Hydrophobin Bilayers. Langmuir, 34(29), 8542-8549. https://doi.org/10.1021/acs.langmuir.8b00575
Hähl, Hendrik ; Vargas, Jose Nabor ; Jung, Michael ; Griffo, Alessandra ; Laaksonen, Päivi ; Lienemann, Michael ; Jacobs, Karin ; Seemann, Ralf ; Fleury, Jean Baptiste. / Adhesion Properties of Freestanding Hydrophobin Bilayers. In: Langmuir. 2018 ; Vol. 34, No. 29. pp. 8542-8549.
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Hähl, H, Vargas, JN, Jung, M, Griffo, A, Laaksonen, P, Lienemann, M, Jacobs, K, Seemann, R & Fleury, JB 2018, 'Adhesion Properties of Freestanding Hydrophobin Bilayers', Langmuir, vol. 34, no. 29, pp. 8542-8549. https://doi.org/10.1021/acs.langmuir.8b00575

Adhesion Properties of Freestanding Hydrophobin Bilayers. / Hähl, Hendrik (Corresponding Author); Vargas, Jose Nabor; Jung, Michael; Griffo, Alessandra; Laaksonen, Päivi; Lienemann, Michael; Jacobs, Karin; Seemann, Ralf; Fleury, Jean Baptiste (Corresponding Author).

In: Langmuir, Vol. 34, No. 29, 24.07.2018, p. 8542-8549.

Research output: Contribution to journalArticleScientificpeer-review

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Hähl H, Vargas JN, Jung M, Griffo A, Laaksonen P, Lienemann M et al. Adhesion Properties of Freestanding Hydrophobin Bilayers. Langmuir. 2018 Jul 24;34(29):8542-8549. https://doi.org/10.1021/acs.langmuir.8b00575