Adhesion studies for probiotics: Need for validation and refinement

S. Blum (Corresponding Author), R. Reniero, E. Schiffrin, Ross Crittenden, Tiina Mattila-Sandholm, A. Ouwehand, Seppo Salminen, Atte von Wright, Maria Saarela, M. Saxelin, K. Collins, L. Morelli

Research output: Contribution to journalReview ArticleScientificpeer-review

86 Citations (Scopus)

Abstract

Adhesion of probiotic bacteria to intestinal epithelial cells is regarded as a prerequisite to exert beneficial health effects. Human intestinal epithelial lines, like Caco-2 or HT-29 cells, have been extensively used to select for adhesive strains in vitro. Adhesion to intestinal mucus has been used to a lesser extent. However, to date, there has been no standardization of the conditions used in in vitro adhesion assays. As a consequence, results obtained in different laboratories using identical strains, but different assay conditions, show great variability. This lack of standardization complicates the interpretation of data and discrepancies emerge on what is meant by effective adhesion. A critical validation of in vitro adhesion is essential for the food industry, using adhesion assays as predicitve screening tools to assess new probiotic strains. In this review we summarize a comparative study on adhesion of the well characterized probiotic bacteria L. johnsonii La1 and L. rhamnosus GG with respect to the influence of bacterial growth conditions, growth phase, buffer, pH, and mucus on adhesion properties. The results are employed in the current scientific discussion to allow a critical evaluation on the reliability of the in vitro assays.
Original languageEnglish
Pages (from-to)405-410
Number of pages6
JournalTrends in Food Science and Technology
Volume10
Issue number12
DOIs
Publication statusPublished - 1999
MoE publication typeA2 Review article in a scientific journal

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Probiotics
probiotics
adhesion
Mucus
Bacteria
HT29 Cells
Food Industry
assays
Growth
mucus
standardization
Adhesives
Buffers
Epithelial Cells
In Vitro Techniques
bacteria
Health
adhesives
microbial growth
food industry

Cite this

Blum, S., Reniero, R., Schiffrin, E., Crittenden, R., Mattila-Sandholm, T., Ouwehand, A., ... Morelli, L. (1999). Adhesion studies for probiotics: Need for validation and refinement. Trends in Food Science and Technology, 10(12), 405-410. https://doi.org/10.1016/S0924-2244(00)00028-5
Blum, S. ; Reniero, R. ; Schiffrin, E. ; Crittenden, Ross ; Mattila-Sandholm, Tiina ; Ouwehand, A. ; Salminen, Seppo ; von Wright, Atte ; Saarela, Maria ; Saxelin, M. ; Collins, K. ; Morelli, L. / Adhesion studies for probiotics : Need for validation and refinement. In: Trends in Food Science and Technology. 1999 ; Vol. 10, No. 12. pp. 405-410.
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Blum, S, Reniero, R, Schiffrin, E, Crittenden, R, Mattila-Sandholm, T, Ouwehand, A, Salminen, S, von Wright, A, Saarela, M, Saxelin, M, Collins, K & Morelli, L 1999, 'Adhesion studies for probiotics: Need for validation and refinement', Trends in Food Science and Technology, vol. 10, no. 12, pp. 405-410. https://doi.org/10.1016/S0924-2244(00)00028-5

Adhesion studies for probiotics : Need for validation and refinement. / Blum, S. (Corresponding Author); Reniero, R.; Schiffrin, E.; Crittenden, Ross; Mattila-Sandholm, Tiina; Ouwehand, A.; Salminen, Seppo; von Wright, Atte; Saarela, Maria; Saxelin, M.; Collins, K.; Morelli, L.

In: Trends in Food Science and Technology, Vol. 10, No. 12, 1999, p. 405-410.

Research output: Contribution to journalReview ArticleScientificpeer-review

TY - JOUR

T1 - Adhesion studies for probiotics

T2 - Need for validation and refinement

AU - Blum, S.

AU - Reniero, R.

AU - Schiffrin, E.

AU - Crittenden, Ross

AU - Mattila-Sandholm, Tiina

AU - Ouwehand, A.

AU - Salminen, Seppo

AU - von Wright, Atte

AU - Saarela, Maria

AU - Saxelin, M.

AU - Collins, K.

AU - Morelli, L.

PY - 1999

Y1 - 1999

N2 - Adhesion of probiotic bacteria to intestinal epithelial cells is regarded as a prerequisite to exert beneficial health effects. Human intestinal epithelial lines, like Caco-2 or HT-29 cells, have been extensively used to select for adhesive strains in vitro. Adhesion to intestinal mucus has been used to a lesser extent. However, to date, there has been no standardization of the conditions used in in vitro adhesion assays. As a consequence, results obtained in different laboratories using identical strains, but different assay conditions, show great variability. This lack of standardization complicates the interpretation of data and discrepancies emerge on what is meant by effective adhesion. A critical validation of in vitro adhesion is essential for the food industry, using adhesion assays as predicitve screening tools to assess new probiotic strains. In this review we summarize a comparative study on adhesion of the well characterized probiotic bacteria L. johnsonii La1 and L. rhamnosus GG with respect to the influence of bacterial growth conditions, growth phase, buffer, pH, and mucus on adhesion properties. The results are employed in the current scientific discussion to allow a critical evaluation on the reliability of the in vitro assays.

AB - Adhesion of probiotic bacteria to intestinal epithelial cells is regarded as a prerequisite to exert beneficial health effects. Human intestinal epithelial lines, like Caco-2 or HT-29 cells, have been extensively used to select for adhesive strains in vitro. Adhesion to intestinal mucus has been used to a lesser extent. However, to date, there has been no standardization of the conditions used in in vitro adhesion assays. As a consequence, results obtained in different laboratories using identical strains, but different assay conditions, show great variability. This lack of standardization complicates the interpretation of data and discrepancies emerge on what is meant by effective adhesion. A critical validation of in vitro adhesion is essential for the food industry, using adhesion assays as predicitve screening tools to assess new probiotic strains. In this review we summarize a comparative study on adhesion of the well characterized probiotic bacteria L. johnsonii La1 and L. rhamnosus GG with respect to the influence of bacterial growth conditions, growth phase, buffer, pH, and mucus on adhesion properties. The results are employed in the current scientific discussion to allow a critical evaluation on the reliability of the in vitro assays.

U2 - 10.1016/S0924-2244(00)00028-5

DO - 10.1016/S0924-2244(00)00028-5

M3 - Review Article

VL - 10

SP - 405

EP - 410

JO - Trends in Food Science and Technology

JF - Trends in Food Science and Technology

SN - 0924-2244

IS - 12

ER -

Blum S, Reniero R, Schiffrin E, Crittenden R, Mattila-Sandholm T, Ouwehand A et al. Adhesion studies for probiotics: Need for validation and refinement. Trends in Food Science and Technology. 1999;10(12):405-410. https://doi.org/10.1016/S0924-2244(00)00028-5