Adsorption of antibodies to a langmuir layer of octadecylamine and the interaction with antigen

Inger Vikholm, Olle Teleman

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

The adsorption of antibodies against C-reactive protein onto a monolayer of octadecylamine has been studied at the air/water interface by following the change in area of the monolayer at different surface pressures. Binding of antibodies was dependent both on the packing of the film and on the antibody concentration in the aqueous subphase. An equilibrium was reached above an antibody concentration of 2 mg/dm3. The monolayer was stabilized by the antibodies. The mixed monolayer could be transferred onto a quartz crystal microbalance. However, the high nonspecific binding of bovine serum albumin makes it difficult to determine the interaction of the antigen with the octadecylamine/antibody layer.

Original languageEnglish
Pages (from-to)125-129
Number of pages5
JournalJournal of Colloid and Interface Science
Volume168
Issue number1
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

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Antigens
Antibodies
Adsorption
Monolayers
Quartz crystal microbalances
Bovine Serum Albumin
C-Reactive Protein
stearylamine
Proteins
Water
Air

Cite this

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title = "Adsorption of antibodies to a langmuir layer of octadecylamine and the interaction with antigen",
abstract = "The adsorption of antibodies against C-reactive protein onto a monolayer of octadecylamine has been studied at the air/water interface by following the change in area of the monolayer at different surface pressures. Binding of antibodies was dependent both on the packing of the film and on the antibody concentration in the aqueous subphase. An equilibrium was reached above an antibody concentration of 2 mg/dm3. The monolayer was stabilized by the antibodies. The mixed monolayer could be transferred onto a quartz crystal microbalance. However, the high nonspecific binding of bovine serum albumin makes it difficult to determine the interaction of the antigen with the octadecylamine/antibody layer.",
author = "Inger Vikholm and Olle Teleman",
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pages = "125--129",
journal = "Journal of Colloid and Interface Science",
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Adsorption of antibodies to a langmuir layer of octadecylamine and the interaction with antigen. / Vikholm, Inger; Teleman, Olle.

In: Journal of Colloid and Interface Science, Vol. 168, No. 1, 1994, p. 125-129.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Adsorption of antibodies to a langmuir layer of octadecylamine and the interaction with antigen

AU - Vikholm, Inger

AU - Teleman, Olle

N1 - Project code: BEL4308

PY - 1994

Y1 - 1994

N2 - The adsorption of antibodies against C-reactive protein onto a monolayer of octadecylamine has been studied at the air/water interface by following the change in area of the monolayer at different surface pressures. Binding of antibodies was dependent both on the packing of the film and on the antibody concentration in the aqueous subphase. An equilibrium was reached above an antibody concentration of 2 mg/dm3. The monolayer was stabilized by the antibodies. The mixed monolayer could be transferred onto a quartz crystal microbalance. However, the high nonspecific binding of bovine serum albumin makes it difficult to determine the interaction of the antigen with the octadecylamine/antibody layer.

AB - The adsorption of antibodies against C-reactive protein onto a monolayer of octadecylamine has been studied at the air/water interface by following the change in area of the monolayer at different surface pressures. Binding of antibodies was dependent both on the packing of the film and on the antibody concentration in the aqueous subphase. An equilibrium was reached above an antibody concentration of 2 mg/dm3. The monolayer was stabilized by the antibodies. The mixed monolayer could be transferred onto a quartz crystal microbalance. However, the high nonspecific binding of bovine serum albumin makes it difficult to determine the interaction of the antigen with the octadecylamine/antibody layer.

U2 - 10.1006/jcis.1994.1401

DO - 10.1006/jcis.1994.1401

M3 - Article

VL - 168

SP - 125

EP - 129

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

IS - 1

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