Adsorption of antibodies to a langmuir layer of octadecylamine and the interaction with antigen

Inger Vikholm, Olle Teleman

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

The adsorption of antibodies against C-reactive protein onto a monolayer of octadecylamine has been studied at the air/water interface by following the change in area of the monolayer at different surface pressures. Binding of antibodies was dependent both on the packing of the film and on the antibody concentration in the aqueous subphase. An equilibrium was reached above an antibody concentration of 2 mg/dm3. The monolayer was stabilized by the antibodies. The mixed monolayer could be transferred onto a quartz crystal microbalance. However, the high nonspecific binding of bovine serum albumin makes it difficult to determine the interaction of the antigen with the octadecylamine/antibody layer.

Original languageEnglish
Pages (from-to)125-129
Number of pages5
JournalJournal of Colloid and Interface Science
Volume168
Issue number1
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

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