Adsorption of hemicellulases onto bleached kraft fibers

Paul Gerber, J. Heitmann, T. Joyce, Johanna Buchert, Matti Siika-aho

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

The presence of a cellulose binding domain was the main factor which influenced the adsorption of two Trichoderma reesei mannanases and a Trichoderma longibrachiatum xylanase onto bleached kraft fibers. The mannanase containing the binding domain adsorbed to a much greater extent than the mannanase and xylanase without binding domains. Once-dried fibers exhibited lower hemicellulase adsorption than the never-dried fibers. The adsorption onto once-dried fibers was only 30–50% the amount adsorbed onto virgin fibers. Adjustment of the ionic strength and pH of treatment was used to modify the charge on the fibers. Ionic strength had opposite effects on mannanase and mannanase core adsorption. Mannanase adsorption increased at high ionic strengths, while the mannanase core adsorption decreased. An increase in pH and fiber charge significantly enhanced the adsorption of xylanase.
Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalJournal of Biotechnology
Volume67
Issue number1
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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Adsorption
Fibers
Ionic strength
Osmolar Concentration
Trichoderma
hemicellulase
Cellulose

Cite this

Gerber, Paul ; Heitmann, J. ; Joyce, T. ; Buchert, Johanna ; Siika-aho, Matti. / Adsorption of hemicellulases onto bleached kraft fibers. In: Journal of Biotechnology. 1999 ; Vol. 67, No. 1. pp. 67-75.
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Adsorption of hemicellulases onto bleached kraft fibers. / Gerber, Paul; Heitmann, J.; Joyce, T.; Buchert, Johanna; Siika-aho, Matti.

In: Journal of Biotechnology, Vol. 67, No. 1, 1999, p. 67-75.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Adsorption of hemicellulases onto bleached kraft fibers

AU - Gerber, Paul

AU - Heitmann, J.

AU - Joyce, T.

AU - Buchert, Johanna

AU - Siika-aho, Matti

PY - 1999

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AB - The presence of a cellulose binding domain was the main factor which influenced the adsorption of two Trichoderma reesei mannanases and a Trichoderma longibrachiatum xylanase onto bleached kraft fibers. The mannanase containing the binding domain adsorbed to a much greater extent than the mannanase and xylanase without binding domains. Once-dried fibers exhibited lower hemicellulase adsorption than the never-dried fibers. The adsorption onto once-dried fibers was only 30–50% the amount adsorbed onto virgin fibers. Adjustment of the ionic strength and pH of treatment was used to modify the charge on the fibers. Ionic strength had opposite effects on mannanase and mannanase core adsorption. Mannanase adsorption increased at high ionic strengths, while the mannanase core adsorption decreased. An increase in pH and fiber charge significantly enhanced the adsorption of xylanase.

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DO - 10.1016/S0168-1656(98)00163-1

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