Affibody conjugation onto bacterial cellulose tubes and bioseparation of human serum albumin

Hannes Orelma, Luis O. Morales, Leena Sisko Johansson, Ingrid C. Hoeger, Ilari Filpponen, Cristina Castro, Orlando J. Rojas, Janne Laine

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

We attached anti-human serum albumin (anti-HSA) affibody ligands on bacterial cellulose (BC) by EDC-NHS-mediated covalent conjugation and physical adsorption and demonstrate their application for tubular biofiltration of blood proteins. The BC fibrils were first modified by carboxymethyl cellulose (CMC) by incorporation of CMC in the BC culture medium, producing in situ a CMC-BC tubular network that was used as biofilter. Alternatively, BC carboxylation was carried out by alkaline TEMPO-NaBr-NaClO oxidation. The BC and modified BC, grown in the form of tubes or flat films, were characterized by using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and conductometric titration. Anti-HSA affibody conjugation onto carboxylated cellulose thin film was verified from sensogram data obtained by surface plasmon resonance (SPR). The HSA specific binding capacity of the carboxylated cellulose conjugated with anti-HSA via EDC-NHS was approximately eight-fold larger when compared to the carboxylated cellulose surface carrying physically adsorbed anti-HSA (∼81 compared to 10 ng cm-2, respectively). Further proof of protein binding via anti-HSA affibody conjugated on tubules of CMC- and TEMPO-oxidized BC was obtained by fluorescence imaging. Specific binding of tagged HSA resulted in a linear increase of fluorescence intensity as a function of tagged HSA concentration in the contacting solution. This journal is

Original languageEnglish
Pages (from-to)51440-51450
Number of pages11
JournalRSC Advances
Volume4
Issue number93
DOIs
Publication statusPublished - 3 Oct 2014
MoE publication typeA1 Journal article-refereed

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Serum Albumin
Cellulose
Carboxymethylcellulose Sodium
Fluorescence
Carboxylation
Cellulose films
Biofiltration
Biofilters
Surface plasmon resonance
Titration
Culture Media
Blood Proteins
X ray photoelectron spectroscopy
Ligands
Imaging techniques
Adsorption
Thin films
Oxidation
Scanning electron microscopy
Blood

Cite this

Orelma, H., Morales, L. O., Johansson, L. S., Hoeger, I. C., Filpponen, I., Castro, C., ... Laine, J. (2014). Affibody conjugation onto bacterial cellulose tubes and bioseparation of human serum albumin. RSC Advances, 4(93), 51440-51450. https://doi.org/10.1039/c4ra08882d
Orelma, Hannes ; Morales, Luis O. ; Johansson, Leena Sisko ; Hoeger, Ingrid C. ; Filpponen, Ilari ; Castro, Cristina ; Rojas, Orlando J. ; Laine, Janne. / Affibody conjugation onto bacterial cellulose tubes and bioseparation of human serum albumin. In: RSC Advances. 2014 ; Vol. 4, No. 93. pp. 51440-51450.
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Orelma, H, Morales, LO, Johansson, LS, Hoeger, IC, Filpponen, I, Castro, C, Rojas, OJ & Laine, J 2014, 'Affibody conjugation onto bacterial cellulose tubes and bioseparation of human serum albumin', RSC Advances, vol. 4, no. 93, pp. 51440-51450. https://doi.org/10.1039/c4ra08882d

Affibody conjugation onto bacterial cellulose tubes and bioseparation of human serum albumin. / Orelma, Hannes; Morales, Luis O.; Johansson, Leena Sisko; Hoeger, Ingrid C.; Filpponen, Ilari; Castro, Cristina; Rojas, Orlando J.; Laine, Janne.

In: RSC Advances, Vol. 4, No. 93, 03.10.2014, p. 51440-51450.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Orelma, Hannes

AU - Morales, Luis O.

AU - Johansson, Leena Sisko

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Orelma H, Morales LO, Johansson LS, Hoeger IC, Filpponen I, Castro C et al. Affibody conjugation onto bacterial cellulose tubes and bioseparation of human serum albumin. RSC Advances. 2014 Oct 3;4(93):51440-51450. https://doi.org/10.1039/c4ra08882d