Abstract
A method for amplification in a microfluidic assay, comprising binding an analyte (90) to a transport element, wherein the transport element comprises a particle (70) and a first antibody element (80) and the analyte is bound to the first antibody element (80); moving the transport element with the analyte (90) bound thereto towards a tracer storage site (10b) comprising tracer elements, wherein a tracer element comprises a first binder element (30), a label element (40) and a second antibody element (50); binding the analyte to the second antibody element (50); moving the transport element with the analyte (90) and the tracer element bound therewith towards a tracer transport site (10b) comprising second binder elements (20); binding the first binder element (30) with a second binder element (20); moving the transport element with the analyte (90) bound thereto towards the tracer storage site (10b) so that the analyte is detached from the second antibody element (50) of the tracer element while the first binder element (30) remains bound to the second binder element (20); andrepeating the steps subsequent to binding the analyte (90) to the transport element a predetermined number of times; wherein the first antibody element (80) has a higher affinity for the analyte (90) than the second antibody element (50). Also a method for detecting an analyte, and an apparatus and a system for a microfluidic assay.
Patent family as of 22.12.2021
EP3884275 A1 20210929 EP20190808864 20191114
FI128458 B 20200529 FI20180006055 20181205
FI20186055 A 20200529 FI20180006055 20181205
US2021292808 AA 20210923 US20210338922 20210604
WO20115356 A1 20200611 WO2019FI50810 20191114
Link to current patent family on right
| Original language | English |
|---|---|
| Patent number | FI20186055 |
| IPC | G01N 35/ 00 A I |
| Priority date | 5/12/18 |
| Publication status | Published - 29 May 2020 |
| MoE publication type | H1 Granted patent |