Agrobacterium rhizogenes-mediated transformation

Root cultures as a source of alkaloids

Research output: Contribution to journalArticleScientificpeer-review

180 Citations (Scopus)

Abstract

Hairy roots, transformed with Agrobacterium rhizogenes, have been found to be suitable for the production of secondary metabolites because of their stable and high productivity in hormone-free culture conditions. A number of plant species including many medicinal plants have been successfully transformed with Agrobacterium rhizogenes. Transformed root cultures have also been found to be a potential source of high-value pharmaceuticals. In this article the most important alkaloids produced by hairy roots are summarised. Several different methods have been used to increase the alkaloid accumulation in hairy root cultures. The selection of high productive root lines based on somaclonal variation offers an interesting option to enhance the productivity. Elicitors and modification of culture conditions have been shown to increase the growth and the alkaloid production in some cases. Genetic engineering is a modern tool to regulate the secondary metabolism also in hairy roots. However, our knowledge on biosynthesis of many alkaloids is still poor. Only a limited number of enzymes and their respective genes which regulate the biosynthetic pathways are fully characterised.
Original languageEnglish
Pages (from-to)859-868
JournalPlanta Medica
Volume68
Issue number10
DOIs
Publication statusPublished - 2002
MoE publication typeNot Eligible

Fingerprint

Agrobacterium
Alkaloids
Productivity
Secondary Metabolism
Genetic engineering
Genetic Engineering
Biosynthesis
Biosynthetic Pathways
Medicinal Plants
Metabolites
Genes
Hormones
Enzymes
Growth
Pharmaceutical Preparations

Keywords

  • Agrobacterium rhizogenes
  • alkaloids
  • genetic engineering
  • hairy roots
  • secondary metabolites
  • metabolites

Cite this

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title = "Agrobacterium rhizogenes-mediated transformation: Root cultures as a source of alkaloids",
abstract = "Hairy roots, transformed with Agrobacterium rhizogenes, have been found to be suitable for the production of secondary metabolites because of their stable and high productivity in hormone-free culture conditions. A number of plant species including many medicinal plants have been successfully transformed with Agrobacterium rhizogenes. Transformed root cultures have also been found to be a potential source of high-value pharmaceuticals. In this article the most important alkaloids produced by hairy roots are summarised. Several different methods have been used to increase the alkaloid accumulation in hairy root cultures. The selection of high productive root lines based on somaclonal variation offers an interesting option to enhance the productivity. Elicitors and modification of culture conditions have been shown to increase the growth and the alkaloid production in some cases. Genetic engineering is a modern tool to regulate the secondary metabolism also in hairy roots. However, our knowledge on biosynthesis of many alkaloids is still poor. Only a limited number of enzymes and their respective genes which regulate the biosynthetic pathways are fully characterised.",
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author = "Nina Sevon and Kirsi-Marja Oksman-Caldentey",
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language = "English",
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Agrobacterium rhizogenes-mediated transformation : Root cultures as a source of alkaloids. / Sevon, Nina; Oksman-Caldentey, Kirsi-Marja.

In: Planta Medica, Vol. 68, No. 10, 2002, p. 859-868.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Agrobacterium rhizogenes-mediated transformation

T2 - Root cultures as a source of alkaloids

AU - Sevon, Nina

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2002

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N2 - Hairy roots, transformed with Agrobacterium rhizogenes, have been found to be suitable for the production of secondary metabolites because of their stable and high productivity in hormone-free culture conditions. A number of plant species including many medicinal plants have been successfully transformed with Agrobacterium rhizogenes. Transformed root cultures have also been found to be a potential source of high-value pharmaceuticals. In this article the most important alkaloids produced by hairy roots are summarised. Several different methods have been used to increase the alkaloid accumulation in hairy root cultures. The selection of high productive root lines based on somaclonal variation offers an interesting option to enhance the productivity. Elicitors and modification of culture conditions have been shown to increase the growth and the alkaloid production in some cases. Genetic engineering is a modern tool to regulate the secondary metabolism also in hairy roots. However, our knowledge on biosynthesis of many alkaloids is still poor. Only a limited number of enzymes and their respective genes which regulate the biosynthetic pathways are fully characterised.

AB - Hairy roots, transformed with Agrobacterium rhizogenes, have been found to be suitable for the production of secondary metabolites because of their stable and high productivity in hormone-free culture conditions. A number of plant species including many medicinal plants have been successfully transformed with Agrobacterium rhizogenes. Transformed root cultures have also been found to be a potential source of high-value pharmaceuticals. In this article the most important alkaloids produced by hairy roots are summarised. Several different methods have been used to increase the alkaloid accumulation in hairy root cultures. The selection of high productive root lines based on somaclonal variation offers an interesting option to enhance the productivity. Elicitors and modification of culture conditions have been shown to increase the growth and the alkaloid production in some cases. Genetic engineering is a modern tool to regulate the secondary metabolism also in hairy roots. However, our knowledge on biosynthesis of many alkaloids is still poor. Only a limited number of enzymes and their respective genes which regulate the biosynthetic pathways are fully characterised.

KW - Agrobacterium rhizogenes

KW - alkaloids

KW - genetic engineering

KW - hairy roots

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KW - metabolites

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