Aldose dehydrogenase-modified carbon paste electrodes as amperometric aldose sensors

Maria Smolander; György Marko-Varga; Lo Gorton

doi:10.1016/0003-2670(94)00469-3

Aldose dehydrogenase-modified carbon paste electrodes as amperometric aldose sensors

Maria Smolander (Corresponding Author), György Marko-Varga, Lo Gorton

Lund University

Research output: Contribution to journal › Article › Scientific › peer-review

32 Citations (Scopus)

Abstract

A biosensor using pyrroloquinoline quinone-dependent aldose dehydrogenase (ALDH) as a biological component was developed and used for the measurement of the aldose sugars xylose and glucose. Different immobilization methods for ALDH in carbon paste were studied. The best electrode performance was obtained when ALDH was adsorbed on the surface of a carbon paste electrode. Several mediator compounds were mixed into the carbon paste. The lowest working potential and highest catalytic current were obtained with dimethylferrocene as a mediator. Both storage and operational stability of the ALDH electrodes could be improved by the application of a membrane consisting of a poly(ester-sulfonic acid) cation-exchanger, Eastman AQ-29D. Application of the membrane reduced the non-specific oxidation of fermentation samples on the electrode surface.

Original language	English
Pages (from-to)	233-240
Journal	Analytica Chimica Acta
Volume	302
Issue number	2-3
DOIs	https://doi.org/10.1016/0003-2670(94)00469-3
Publication status	Published - 1995
MoE publication type	A1 Journal article-refereed

Access to Document

10.1016/0003-2670(94)00469-3

Cite this

@article{0af4b7ea0ecb4e209dc3f83a8f7dcb6a,

title = "Aldose dehydrogenase-modified carbon paste electrodes as amperometric aldose sensors",

abstract = "A biosensor using pyrroloquinoline quinone-dependent aldose dehydrogenase (ALDH) as a biological component was developed and used for the measurement of the aldose sugars xylose and glucose. Different immobilization methods for ALDH in carbon paste were studied. The best electrode performance was obtained when ALDH was adsorbed on the surface of a carbon paste electrode. Several mediator compounds were mixed into the carbon paste. The lowest working potential and highest catalytic current were obtained with dimethylferrocene as a mediator. Both storage and operational stability of the ALDH electrodes could be improved by the application of a membrane consisting of a poly(ester-sulfonic acid) cation-exchanger, Eastman AQ-29D. Application of the membrane reduced the non-specific oxidation of fermentation samples on the electrode surface.",

author = "Maria Smolander and Gy{\"o}rgy Marko-Varga and Lo Gorton",

note = "Project code: B5SU00064",

year = "1995",

doi = "10.1016/0003-2670(94)00469-3",

language = "English",

volume = "302",

pages = "233--240",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier",

number = "2-3",

}

TY - JOUR

T1 - Aldose dehydrogenase-modified carbon paste electrodes as amperometric aldose sensors

AU - Smolander, Maria

AU - Marko-Varga, György

AU - Gorton, Lo

N1 - Project code: B5SU00064

PY - 1995

Y1 - 1995

N2 - A biosensor using pyrroloquinoline quinone-dependent aldose dehydrogenase (ALDH) as a biological component was developed and used for the measurement of the aldose sugars xylose and glucose. Different immobilization methods for ALDH in carbon paste were studied. The best electrode performance was obtained when ALDH was adsorbed on the surface of a carbon paste electrode. Several mediator compounds were mixed into the carbon paste. The lowest working potential and highest catalytic current were obtained with dimethylferrocene as a mediator. Both storage and operational stability of the ALDH electrodes could be improved by the application of a membrane consisting of a poly(ester-sulfonic acid) cation-exchanger, Eastman AQ-29D. Application of the membrane reduced the non-specific oxidation of fermentation samples on the electrode surface.

AB - A biosensor using pyrroloquinoline quinone-dependent aldose dehydrogenase (ALDH) as a biological component was developed and used for the measurement of the aldose sugars xylose and glucose. Different immobilization methods for ALDH in carbon paste were studied. The best electrode performance was obtained when ALDH was adsorbed on the surface of a carbon paste electrode. Several mediator compounds were mixed into the carbon paste. The lowest working potential and highest catalytic current were obtained with dimethylferrocene as a mediator. Both storage and operational stability of the ALDH electrodes could be improved by the application of a membrane consisting of a poly(ester-sulfonic acid) cation-exchanger, Eastman AQ-29D. Application of the membrane reduced the non-specific oxidation of fermentation samples on the electrode surface.

U2 - 10.1016/0003-2670(94)00469-3

DO - 10.1016/0003-2670(94)00469-3

M3 - Article

SN - 0003-2670

VL - 302

SP - 233

EP - 240

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 2-3

ER -

Aldose dehydrogenase-modified carbon paste electrodes as amperometric aldose sensors

Abstract

Access to Document

Fingerprint

Cite this