TY - JOUR
T1 - Allocation of rhodamine-loaded nanocapsules from blood circulatory system to adjacent tissues assessed in vivo by fluorescence spectroscopy
AU - Tarakanchikova, Yana
AU - Stelmashchuk, Olga
AU - Seryogina, Evgeniya
AU - Piavchenko, Gennadii
AU - Zherebtsov, Evgeny
AU - Dunaev, Andrey
AU - Popov, Alexey
AU - Meglinski, Igor
PY - 2018/10
Y1 - 2018/10
N2 - Modern fluorescent modalities play an important role in the functional diagnostic of various physiological processes in living tissues. Utilizing the fluorescence spectroscopy approach we observe the circulation of fluorescent-labelled nanocapsules with rhodamine tetramethylrhodamine in a microcirculatory blood system. The measurements were conducted transcutaneously on the surface of healthy Wistar rat thighs in vivo. The administration of the preparation capsule suspension with a rhodamine concentration of 5 mg kg-1 of the animal weight resulted in a two-fold increase of fluorescence intensity relative to the baseline level. The dissemination of nanocapsules in the adjacent tissues via the circulatory system was observed and assessed quantitatively. The approach can be used for the transdermal assessment of rhodamine-loaded capsules in vivo.
AB - Modern fluorescent modalities play an important role in the functional diagnostic of various physiological processes in living tissues. Utilizing the fluorescence spectroscopy approach we observe the circulation of fluorescent-labelled nanocapsules with rhodamine tetramethylrhodamine in a microcirculatory blood system. The measurements were conducted transcutaneously on the surface of healthy Wistar rat thighs in vivo. The administration of the preparation capsule suspension with a rhodamine concentration of 5 mg kg-1 of the animal weight resulted in a two-fold increase of fluorescence intensity relative to the baseline level. The dissemination of nanocapsules in the adjacent tissues via the circulatory system was observed and assessed quantitatively. The approach can be used for the transdermal assessment of rhodamine-loaded capsules in vivo.
KW - blood microcirculation
KW - drug delivery
KW - fluorescence spectroscopy
KW - fluorescent-labelled particles
KW - Nanocomposite polymeric capsules
KW - optical measurements in vivo
UR - http://www.scopus.com/inward/record.url?scp=85054300938&partnerID=8YFLogxK
U2 - 10.1088/1612-202X/aad857
DO - 10.1088/1612-202X/aad857
M3 - Article
AN - SCOPUS:85054300938
SN - 1612-2011
VL - 15
JO - Laser Physics Letters
JF - Laser Physics Letters
IS - 10
M1 - 105601
ER -