Abstract
Modern fluorescent modalities play an important role in the functional diagnostic of various physiological processes in living tissues. Utilizing the fluorescence spectroscopy approach we observe the circulation of fluorescent-labelled nanocapsules with rhodamine tetramethylrhodamine in a microcirculatory blood system. The measurements were conducted transcutaneously on the surface of healthy Wistar rat thighs in vivo. The administration of the preparation capsule suspension with a rhodamine concentration of 5 mg kg-1 of the animal weight resulted in a two-fold increase of fluorescence intensity relative to the baseline level. The dissemination of nanocapsules in the adjacent tissues via the circulatory system was observed and assessed quantitatively. The approach can be used for the transdermal assessment of rhodamine-loaded capsules in vivo.
| Original language | English |
|---|---|
| Article number | 105601 |
| Journal | Laser Physics Letters |
| Volume | 15 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2018 |
| MoE publication type | A1 Journal article-refereed |
Funding
This work was supported by the Russian Foundation on Innovations U.M.N.I.K., the 2017 SPIE Optics and Photonics Education Scholarship, the CIMO Fellowship (TM-15-9729, YT), the EDUFI Fellowship (TM-17-10389, YT), the MEPhI Academic Excellence Project (contract no. 02.a03.21.0005, IM), the Tomsk State University Competitiveness Improvement Programme (IM), and the Academy of Finland (grant no. 311698, IM, grant 290596, AP). EZ kindly acknowledges the funding from the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement no. 703145. AD and ES kindly acknowledges the funding by the Russian Science Foundation under project №18-15-00201.
Keywords
- blood microcirculation
- drug delivery
- fluorescence spectroscopy
- fluorescent-labelled particles
- Nanocomposite polymeric capsules
- optical measurements in vivo