Alternative methods for sampling from surfaces

Satu Salo, Erna Storgårds, Gun Wirtanen

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Detection of attached microorganisms and biofilms on surfaces is essential in assessing process hygiene. Alternative methods to assess surface hygiene were studied. As a sampling method, ultrasonication was found to be more effective than swabbing. Swabbing can, however, be improved using wetting agents. The wetting agent breaks the biofilm and reveals the bacteria in it without affecting the survival rate of the bacteria. These agents can be applied either by spraying directly onto the surface or by moistening the swabs before sampling. The viability of surface-bound microorganisms and/or the quantity of biofilm was estimated by ATP bioluminescence, by protein residue detection, by impedimetry and by epifluorescence microscopy. The ATP method and detection of protein are easily applicable for industrial use, whereas impedimetry and epifluorescence microscopy are indispensable research tools. These techniques offer many benefits compared to conventional methods, although some limitations were also noticed.
Original languageEnglish
Title of host publication30th R3-Nordic Contamination Control Symposium
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages187-198
ISBN (Print)951-38-5268-7
Publication statusPublished - 1999
MoE publication typeA4 Article in a conference publication
Event30th R3-Nordic Contamination Control Symposium
- Helsinki, Finland
Duration: 30 May 19992 Jun 1999

Publication series

NameVTT Symposium
PublisherVTT
Number193
ISSN (Print)0357-9387
ISSN (Electronic)1455-0873

Conference

Conference30th R3-Nordic Contamination Control Symposium
CountryFinland
CityHelsinki
Period30/05/992/06/99

Fingerprint

Biofilms
Wetting Agents
Sampling
Microorganisms
Bacteria
Microscopic examination
Adenosine Triphosphate
Bioluminescence
Spraying
Wetting
Proteins

Cite this

Salo, S., Storgårds, E., & Wirtanen, G. (1999). Alternative methods for sampling from surfaces. In 30th R3-Nordic Contamination Control Symposium (pp. 187-198). Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 193
Salo, Satu ; Storgårds, Erna ; Wirtanen, Gun. / Alternative methods for sampling from surfaces. 30th R3-Nordic Contamination Control Symposium. Espoo : VTT Technical Research Centre of Finland, 1999. pp. 187-198 (VTT Symposium; No. 193).
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Salo, S, Storgårds, E & Wirtanen, G 1999, Alternative methods for sampling from surfaces. in 30th R3-Nordic Contamination Control Symposium. VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 193, pp. 187-198, 30th R3-Nordic Contamination Control Symposium
, Helsinki, Finland, 30/05/99.

Alternative methods for sampling from surfaces. / Salo, Satu; Storgårds, Erna; Wirtanen, Gun.

30th R3-Nordic Contamination Control Symposium. Espoo : VTT Technical Research Centre of Finland, 1999. p. 187-198 (VTT Symposium; No. 193).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

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AB - Detection of attached microorganisms and biofilms on surfaces is essential in assessing process hygiene. Alternative methods to assess surface hygiene were studied. As a sampling method, ultrasonication was found to be more effective than swabbing. Swabbing can, however, be improved using wetting agents. The wetting agent breaks the biofilm and reveals the bacteria in it without affecting the survival rate of the bacteria. These agents can be applied either by spraying directly onto the surface or by moistening the swabs before sampling. The viability of surface-bound microorganisms and/or the quantity of biofilm was estimated by ATP bioluminescence, by protein residue detection, by impedimetry and by epifluorescence microscopy. The ATP method and detection of protein are easily applicable for industrial use, whereas impedimetry and epifluorescence microscopy are indispensable research tools. These techniques offer many benefits compared to conventional methods, although some limitations were also noticed.

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Salo S, Storgårds E, Wirtanen G. Alternative methods for sampling from surfaces. In 30th R3-Nordic Contamination Control Symposium. Espoo: VTT Technical Research Centre of Finland. 1999. p. 187-198. (VTT Symposium; No. 193).