Amino acid-derived betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet-A nontargeted metabolomics study

Jenna Pekkinen (Corresponding Author), Natalia Rosa-Sibakov, Valerie Micard, Pekka Keski-Rahkonen, Marko Lehtonen, Kaisa Poutanen, Hannu Mykkänen, Kati Hanhineva

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

Scope: Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results: Nontargeted hydrophilic interaction chromatography-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from three groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived betaines after both rye diets. These included proline betaine, alanine betaine, valine betaine, phenylalanine betaine, pipecolic acid betaine, and trigonelline, but not glycine betaine. Furthermore, bioprocessing may have improved the bioavailability of rye-derived phytochemicals, as higher increase in, e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion: Urinary excretion of various betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.
Original languageEnglish
Pages (from-to)1550-1562
JournalMolecular Nutrition and Food Research
Volume59
Issue number8
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

rye bran
Betaine
Metabolomics
betaine
metabolomics
High Fat Diet
high fat diet
Amino Acids
amino acids
mice
Phytochemicals
bioprocessing
phytopharmaceuticals
Biological Availability
bioavailability
ferulic acid
Diet
rye
urine
pipecolic acid

Keywords

  • betaines
  • dietary fiber
  • HILIC
  • metabolite profiling
  • rye

Cite this

Pekkinen, Jenna ; Rosa-Sibakov, Natalia ; Micard, Valerie ; Keski-Rahkonen, Pekka ; Lehtonen, Marko ; Poutanen, Kaisa ; Mykkänen, Hannu ; Hanhineva, Kati. / Amino acid-derived betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet-A nontargeted metabolomics study. In: Molecular Nutrition and Food Research. 2015 ; Vol. 59, No. 8. pp. 1550-1562.
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abstract = "Scope: Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results: Nontargeted hydrophilic interaction chromatography-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from three groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived betaines after both rye diets. These included proline betaine, alanine betaine, valine betaine, phenylalanine betaine, pipecolic acid betaine, and trigonelline, but not glycine betaine. Furthermore, bioprocessing may have improved the bioavailability of rye-derived phytochemicals, as higher increase in, e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion: Urinary excretion of various betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.",
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Amino acid-derived betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet-A nontargeted metabolomics study. / Pekkinen, Jenna (Corresponding Author); Rosa-Sibakov, Natalia; Micard, Valerie; Keski-Rahkonen, Pekka; Lehtonen, Marko; Poutanen, Kaisa; Mykkänen, Hannu; Hanhineva, Kati.

In: Molecular Nutrition and Food Research, Vol. 59, No. 8, 2015, p. 1550-1562.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Amino acid-derived betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet-A nontargeted metabolomics study

AU - Pekkinen, Jenna

AU - Rosa-Sibakov, Natalia

AU - Micard, Valerie

AU - Keski-Rahkonen, Pekka

AU - Lehtonen, Marko

AU - Poutanen, Kaisa

AU - Mykkänen, Hannu

AU - Hanhineva, Kati

PY - 2015

Y1 - 2015

N2 - Scope: Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results: Nontargeted hydrophilic interaction chromatography-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from three groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived betaines after both rye diets. These included proline betaine, alanine betaine, valine betaine, phenylalanine betaine, pipecolic acid betaine, and trigonelline, but not glycine betaine. Furthermore, bioprocessing may have improved the bioavailability of rye-derived phytochemicals, as higher increase in, e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion: Urinary excretion of various betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

AB - Scope: Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results: Nontargeted hydrophilic interaction chromatography-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from three groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived betaines after both rye diets. These included proline betaine, alanine betaine, valine betaine, phenylalanine betaine, pipecolic acid betaine, and trigonelline, but not glycine betaine. Furthermore, bioprocessing may have improved the bioavailability of rye-derived phytochemicals, as higher increase in, e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion: Urinary excretion of various betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

KW - betaines

KW - dietary fiber

KW - HILIC

KW - metabolite profiling

KW - rye

U2 - 10.1002/mnfr.201500066

DO - 10.1002/mnfr.201500066

M3 - Article

VL - 59

SP - 1550

EP - 1562

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

IS - 8

ER -