TY - JOUR
T1 - An amperometric biosensor of L-fucose in urine for the first screening test of cancer
AU - Takeda, Kouta
AU - Kusuoka, Ryo
AU - Inukai, Misaki
AU - Igarashi, Kiyohiko
AU - Ohno, Hiroyuki
AU - Nakamura, Nobuhumi
N1 - Funding Information:
Funding was provided by a Grant-in-Aid for Young Scientific Research (B) ( 17 K17703 to K.T.) and a Grant-in-Aid for Scientific Research (B) ( 19H03013 to K.I.) from Japan Society for the Promotion of Science (JSPS) . This research was also supported by a Grant-in-Aid for Innovative Areas from the Japanese Ministry of Education, Culture, Sports, and Technology (MEXT) (No. 18H05494 to K.I.). K.I. thanks to the Business Finland (BF, Previously the Finnish Funding Agency for Innovation (TEKES)) for the support of the Finland Distinguished Professor (FiDiPro) Program “Advanced approaches for enzymatic biomass utilization and modification (BioAD).”
PY - 2021/2/15
Y1 - 2021/2/15
N2 - Quantitative routine detection of fucose, which is a cancer marker, in urine is effective for the preliminary screening of cancer. Amperometric biosensing methods have the advantage of being simple, rapid, and precise for urinalysis. However, coexisting electroactive interferences such as ascorbic acid (AA), dopamine (DA), and uric acid (UA) prevent accurate measurements. In this work, an amperometric L-fucose biosensor unaffected by interferences was developed and utilizes direct electron transfer type bioelectrocatalysis of pyrroloquinoline quinone (PQQ)-dependent pyranose dehydrogenase from Coprinopsis cinerea (CcPDH). The isolated PQQ domain from CcPDH was immobilized on gold nanoparticle (AuNP)-modified electrodes, which obtained a catalytic current at a lower potential than the oxidation potential of the interfering compounds. Applying an operating potential of −0.1 V vs. Ag|AgCl (3 M NaCl) enabled the detection of L-fucose while completely eliminating the oxidation of AA, DA, and UA on the electrodes. The increase in the specific area of the electrodes by increasing the AuNP drop-casting time resulted in an improvement in the sensor performance. The biosensor exhibited a linear range for L-fucose detection between 0.1 mM and 1 mM (R2 = 0.9996), including a cut-off value, the sensitivity was 3.12 ± 0.05 μA mM−1 cm−2, and the detection limit was 13.6 μM at a signal-to-noise ratio of three. The biosensor can be used to quantify the concentration of L-fucose at physiological levels and does not require urine preprocessing, making it applicable to practical use for point-of-care testing with urine.
AB - Quantitative routine detection of fucose, which is a cancer marker, in urine is effective for the preliminary screening of cancer. Amperometric biosensing methods have the advantage of being simple, rapid, and precise for urinalysis. However, coexisting electroactive interferences such as ascorbic acid (AA), dopamine (DA), and uric acid (UA) prevent accurate measurements. In this work, an amperometric L-fucose biosensor unaffected by interferences was developed and utilizes direct electron transfer type bioelectrocatalysis of pyrroloquinoline quinone (PQQ)-dependent pyranose dehydrogenase from Coprinopsis cinerea (CcPDH). The isolated PQQ domain from CcPDH was immobilized on gold nanoparticle (AuNP)-modified electrodes, which obtained a catalytic current at a lower potential than the oxidation potential of the interfering compounds. Applying an operating potential of −0.1 V vs. Ag|AgCl (3 M NaCl) enabled the detection of L-fucose while completely eliminating the oxidation of AA, DA, and UA on the electrodes. The increase in the specific area of the electrodes by increasing the AuNP drop-casting time resulted in an improvement in the sensor performance. The biosensor exhibited a linear range for L-fucose detection between 0.1 mM and 1 mM (R2 = 0.9996), including a cut-off value, the sensitivity was 3.12 ± 0.05 μA mM−1 cm−2, and the detection limit was 13.6 μM at a signal-to-noise ratio of three. The biosensor can be used to quantify the concentration of L-fucose at physiological levels and does not require urine preprocessing, making it applicable to practical use for point-of-care testing with urine.
KW - Ascorbic acid interference
KW - Bioelectrocatalysis
KW - Direct electron transfer
KW - Enzyme biosensor
KW - Gold nanoparticle
KW - PQQ
UR - http://www.scopus.com/inward/record.url?scp=85097225353&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2020.112831
DO - 10.1016/j.bios.2020.112831
M3 - Article
C2 - 33288426
AN - SCOPUS:85097225353
SN - 0956-5663
VL - 174
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
M1 - 112831
ER -