An extension of the host range of phage A: Salmonella typhimurium as a model: Dissertation

Anu Harkki

Research output: ThesisDissertationCollection of Articles

Abstract

In this study the host range of phage A has been extended to Salmonella typhimurium. All tested derivatives of A could both produce plaques on this strain at frequencies comparable to Escherichia coli and Iysogenize this strain. This was achieved by including the E. coli genes lamB (receptor for 1) and nusA (a host factor of transcription complex) into S. typhimurium. To facilitate strain constructions a vector expressing both of these E. coli genes was constructed. The feasibility of this vector was studied in S. typhimurium by isolating in vivo gene fusions to an outer membrane porin gene with phage A vehicles. An expression vector for introduction of,' receptor function into other enteric bacteria was constructed. The ability of this vector to express lamB was verified in several enteric bacteria: E. coli, S. typhimurium, Vibrio cholerae and Erwinia carotovora. The function of LamB as a A receptor was determined in V. cholerae by studying the ability of these new V. cholerae derivatives to support cosmid transduction with 1 vehicles. In conclusion, it has been shown that the host range of A can be extended and furthermore that the ability of A to serve as a vehicle for transfer of genetic elements can be introduced via /lamBEC into variety of enteric bacteria.
Original languageEnglish
QualificationDoctor Degree
Awarding Institution
  • University of Helsinki
Supervisors/Advisors
  • Palva, Tapio, Supervisor, External person
Award date5 Jun 1987
Place of PublicationEspoo
Publisher
Print ISBNs951-38-2876-X
Publication statusPublished - 1987
MoE publication typeG5 Doctoral dissertation (article)

Keywords

  • bacteriophages
  • Salmonella typhimurium
  • genetic engineering

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    Harkki, A. (1987). An extension of the host range of phage A: Salmonella typhimurium as a model: Dissertation. VTT Technical Research Centre of Finland.