An integrated young interferometer based on UV-imprinted polymer waveguides for label-free biosensing applications

M. Wang (Corresponding Author), Jussi Hiltunen, Christina Liedert, Leena Hakalahti, R. Myllylä

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

We demonstrate a polymer rib waveguide Young interferometer sensor fabricated by UV-imprinting. An inverted rib waveguide structure was utilized in order to simplify the fabrication process. In this configuration grooves are formed on the under cladding layer by UV-imprinting and core material is spin coated on top to fill the grooves. Glucose water solution was used to characterize the sensor response against ambient refractive index changes. The sensing responses correspond linearly with the refractive index change of glucose solutions with a detection limit of about 10-5. To verify the capability of the polymer sensor for biosensing, an immunoassay was performed with c-reactive protein (CRP) and human CRP specific antibody adsorbed on the waveguide surface as a receptor. The CRP solution in PBS (phosphate buffered saline) buffer with a concentration of 2 µg/ml (16 nM) resulted in an obvious response which was over a couple hundred times of the noise level. Based on these values, a detection limit of about 2.4 pg/mm2 was found for the surface sensing of molecular adsorption. With the proposed waveguide configuration, the fabrication of polymer sensors can be ultimately transferred to roll-to-roll mass production to produce low-cost disposable sensors.
Original languageEnglish
JournalJournal of the European Optical Society: Rapid Publications
Volume7
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

interferometers
waveguides
sensors
polymers
proteins
glucose
grooves
refractivity
immunoassay
fabrication
configurations
antibodies
phosphates
buffers
adsorption
water

Keywords

  • planar waveguide biosensors
  • polymer waveguide
  • UV-imprinting lithography
  • young interferometer

Cite this

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title = "An integrated young interferometer based on UV-imprinted polymer waveguides for label-free biosensing applications",
abstract = "We demonstrate a polymer rib waveguide Young interferometer sensor fabricated by UV-imprinting. An inverted rib waveguide structure was utilized in order to simplify the fabrication process. In this configuration grooves are formed on the under cladding layer by UV-imprinting and core material is spin coated on top to fill the grooves. Glucose water solution was used to characterize the sensor response against ambient refractive index changes. The sensing responses correspond linearly with the refractive index change of glucose solutions with a detection limit of about 10-5. To verify the capability of the polymer sensor for biosensing, an immunoassay was performed with c-reactive protein (CRP) and human CRP specific antibody adsorbed on the waveguide surface as a receptor. The CRP solution in PBS (phosphate buffered saline) buffer with a concentration of 2 µg/ml (16 nM) resulted in an obvious response which was over a couple hundred times of the noise level. Based on these values, a detection limit of about 2.4 pg/mm2 was found for the surface sensing of molecular adsorption. With the proposed waveguide configuration, the fabrication of polymer sensors can be ultimately transferred to roll-to-roll mass production to produce low-cost disposable sensors.",
keywords = "planar waveguide biosensors, polymer waveguide, UV-imprinting lithography, young interferometer",
author = "M. Wang and Jussi Hiltunen and Christina Liedert and Leena Hakalahti and R. Myllyl{\"a}",
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TY - JOUR

T1 - An integrated young interferometer based on UV-imprinted polymer waveguides for label-free biosensing applications

AU - Wang, M.

AU - Hiltunen, Jussi

AU - Liedert, Christina

AU - Hakalahti, Leena

AU - Myllylä, R.

PY - 2012

Y1 - 2012

N2 - We demonstrate a polymer rib waveguide Young interferometer sensor fabricated by UV-imprinting. An inverted rib waveguide structure was utilized in order to simplify the fabrication process. In this configuration grooves are formed on the under cladding layer by UV-imprinting and core material is spin coated on top to fill the grooves. Glucose water solution was used to characterize the sensor response against ambient refractive index changes. The sensing responses correspond linearly with the refractive index change of glucose solutions with a detection limit of about 10-5. To verify the capability of the polymer sensor for biosensing, an immunoassay was performed with c-reactive protein (CRP) and human CRP specific antibody adsorbed on the waveguide surface as a receptor. The CRP solution in PBS (phosphate buffered saline) buffer with a concentration of 2 µg/ml (16 nM) resulted in an obvious response which was over a couple hundred times of the noise level. Based on these values, a detection limit of about 2.4 pg/mm2 was found for the surface sensing of molecular adsorption. With the proposed waveguide configuration, the fabrication of polymer sensors can be ultimately transferred to roll-to-roll mass production to produce low-cost disposable sensors.

AB - We demonstrate a polymer rib waveguide Young interferometer sensor fabricated by UV-imprinting. An inverted rib waveguide structure was utilized in order to simplify the fabrication process. In this configuration grooves are formed on the under cladding layer by UV-imprinting and core material is spin coated on top to fill the grooves. Glucose water solution was used to characterize the sensor response against ambient refractive index changes. The sensing responses correspond linearly with the refractive index change of glucose solutions with a detection limit of about 10-5. To verify the capability of the polymer sensor for biosensing, an immunoassay was performed with c-reactive protein (CRP) and human CRP specific antibody adsorbed on the waveguide surface as a receptor. The CRP solution in PBS (phosphate buffered saline) buffer with a concentration of 2 µg/ml (16 nM) resulted in an obvious response which was over a couple hundred times of the noise level. Based on these values, a detection limit of about 2.4 pg/mm2 was found for the surface sensing of molecular adsorption. With the proposed waveguide configuration, the fabrication of polymer sensors can be ultimately transferred to roll-to-roll mass production to produce low-cost disposable sensors.

KW - planar waveguide biosensors

KW - polymer waveguide

KW - UV-imprinting lithography

KW - young interferometer

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M3 - Article

VL - 7

JO - Journal of the European Optical Society: Rapid Publications

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SN - 1990-2573

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