An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study

Stine M. Ulven; Kirsten B. Holven; Amanda Rundblad; Mari C. W. Myhrstad; Lena Leder; Ingrid Dahlman; Vanessa D. De Mello; Ursula Schwab; Carsten Carlberg; Jussi Pihlajamäki; Kjeld Hermansen; Lars O. Dragsted; Ingibjörg Gunnarsdottir; Lieselotte Cloetens; Björn Åkesson; Fredrik Rosqvist; Janne Hukkanen; Karl-heinz Herzig; Markku J Savolainen; Ulf Risérus; Inga Thorsdottir; Kaisa S Poutanen; Peter Arner; Matti Uusitupa; Marjukka Kolehmainen

doi:10.3390/nu11122932

An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study

Stine M. Ulven (Corresponding Author), Kirsten B. Holven, Amanda Rundblad, Mari C. W. Myhrstad, Lena Leder, Ingrid Dahlman, Vanessa D. De Mello, Ursula Schwab, Carsten Carlberg, Jussi Pihlajamäki, Kjeld Hermansen, Lars O. Dragsted, Ingibjörg Gunnarsdottir, Lieselotte Cloetens, Björn Åkesson, Fredrik Rosqvist, Janne Hukkanen, Karl-heinz Herzig, Markku J Savolainen, Ulf RisérusInga Thorsdottir, Kaisa S Poutanen, Peter Arner, Matti Uusitupa, Marjukka Kolehmainen

BA34 Industrial biotechnology and food solutions

Research output: Contribution to journal › Article › Scientific › peer-review

2 Citations (Scopus)

Abstract

A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor necrosis factor (TNF) receptor superfamily member 1A (TNFRSF1A) was down-regulated (p = 0.004), whereas the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit, RELA proto-oncogene, was up-regulated (p = 0.016) in the ND group compared to the CD group. In conclusion, intake of an ND in individuals with the MetS may affect immune function.

Original language	English
Article number	2932
Journal	Nutrients
Volume	11
Issue number	12
DOIs	https://doi.org/10.3390/nu11122932
Publication status	Published - Dec 2019
MoE publication type	A1 Journal article-refereed

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Ulven, S. M., Holven, K. B., Rundblad, A., Myhrstad, M. C. W., Leder, L., Dahlman, I., Mello, V. D. D., Schwab, U., Carlberg, C., Pihlajamäki, J., Hermansen, K., Dragsted, L. O., Gunnarsdottir, I., Cloetens, L., Åkesson, B., Rosqvist, F., Hukkanen, J., Herzig, K., Savolainen, M. J., ... Kolehmainen, M. (2019). An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study. Nutrients, 11(12), [2932]. https://doi.org/10.3390/nu11122932

Ulven, Stine M. ; Holven, Kirsten B. ; Rundblad, Amanda ; Myhrstad, Mari C. W. ; Leder, Lena ; Dahlman, Ingrid ; Mello, Vanessa D. De ; Schwab, Ursula ; Carlberg, Carsten ; Pihlajamäki, Jussi ; Hermansen, Kjeld ; Dragsted, Lars O. ; Gunnarsdottir, Ingibjörg ; Cloetens, Lieselotte ; Åkesson, Björn ; Rosqvist, Fredrik ; Hukkanen, Janne ; Herzig, Karl-heinz ; Savolainen, Markku J ; Risérus, Ulf ; Thorsdottir, Inga ; Poutanen, Kaisa S ; Arner, Peter ; Uusitupa, Matti ; Kolehmainen, Marjukka. / An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study. In: Nutrients. 2019 ; Vol. 11, No. 12.

@article{4ca1fa7836d349b9aab23e3d9d1efb76,

title = "An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study",

abstract = "A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor necrosis factor (TNF) receptor superfamily member 1A (TNFRSF1A) was down-regulated (p = 0.004), whereas the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit, RELA proto-oncogene, was up-regulated (p = 0.016) in the ND group compared to the CD group. In conclusion, intake of an ND in individuals with the MetS may affect immune function.",

author = "Ulven, {Stine M.} and Holven, {Kirsten B.} and Amanda Rundblad and Myhrstad, {Mari C. W.} and Lena Leder and Ingrid Dahlman and Mello, {Vanessa D. De} and Ursula Schwab and Carsten Carlberg and Jussi Pihlajam{\"a}ki and Kjeld Hermansen and Dragsted, {Lars O.} and Ingibj{\"o}rg Gunnarsdottir and Lieselotte Cloetens and Bj{\"o}rn {\AA}kesson and Fredrik Rosqvist and Janne Hukkanen and Karl-heinz Herzig and Savolainen, {Markku J} and Ulf Ris{\'e}rus and Inga Thorsdottir and Poutanen, {Kaisa S} and Peter Arner and Matti Uusitupa and Marjukka Kolehmainen",

note = "Funding Information: Funding: This research was funded by the NordForsk Nordic Centre of Excellence in Food, Nutrition and Health project 070014 [SYSDIET (Systems Biology in Controlled Dietary Interventions and Cohort Studies)] and further, the University of Oslo (Norway), Throne Holst Foundation (Norway), Academy of Finland, Swedish Research council, Svenska Diabetesf{\"o}rbundet, SRP Diabetes, Finnish Diabetes Research Foundation, Finnish Foundation for Cardiovascular Research, the Sigrid Juselius Foundation, EVO funding from Kuopio University Hospital (Finland), the Druvan Foundation, Sk{\aa}ne University Hospital, the Heart-Lung Foundation, Diabetesfonden and Foundation Cerealia (Sweden), the Agricultural Productivity Fund, and the Research Fund of the University of Iceland (Iceland). Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

year = "2019",

month = dec,

doi = "10.3390/nu11122932",

language = "English",

volume = "11",

journal = "Nutrients",

issn = "2072-6643",

publisher = "MDPI",

number = "12",

}

Ulven, SM, Holven, KB, Rundblad, A, Myhrstad, MCW, Leder, L, Dahlman, I, Mello, VDD, Schwab, U, Carlberg, C, Pihlajamäki, J, Hermansen, K, Dragsted, LO, Gunnarsdottir, I, Cloetens, L, Åkesson, B, Rosqvist, F, Hukkanen, J, Herzig, K, Savolainen, MJ, Risérus, U, Thorsdottir, I, Poutanen, KS, Arner, P, Uusitupa, M & Kolehmainen, M 2019, 'An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study', Nutrients, vol. 11, no. 12, 2932. https://doi.org/10.3390/nu11122932

An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study. / Ulven, Stine M. (Corresponding Author); Holven, Kirsten B.; Rundblad, Amanda; Myhrstad, Mari C. W.; Leder, Lena; Dahlman, Ingrid; Mello, Vanessa D. De; Schwab, Ursula; Carlberg, Carsten; Pihlajamäki, Jussi; Hermansen, Kjeld; Dragsted, Lars O.; Gunnarsdottir, Ingibjörg; Cloetens, Lieselotte; Åkesson, Björn; Rosqvist, Fredrik; Hukkanen, Janne; Herzig, Karl-heinz; Savolainen, Markku J; Risérus, Ulf; Thorsdottir, Inga; Poutanen, Kaisa S; Arner, Peter; Uusitupa, Matti; Kolehmainen, Marjukka.

In: Nutrients, Vol. 11, No. 12, 2932, 12.2019.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study

AU - Ulven, Stine M.

AU - Holven, Kirsten B.

AU - Rundblad, Amanda

AU - Myhrstad, Mari C. W.

AU - Leder, Lena

AU - Dahlman, Ingrid

AU - Mello, Vanessa D. De

AU - Schwab, Ursula

AU - Carlberg, Carsten

AU - Pihlajamäki, Jussi

AU - Hermansen, Kjeld

AU - Dragsted, Lars O.

AU - Gunnarsdottir, Ingibjörg

AU - Cloetens, Lieselotte

AU - Åkesson, Björn

AU - Rosqvist, Fredrik

AU - Hukkanen, Janne

AU - Herzig, Karl-heinz

AU - Savolainen, Markku J

AU - Risérus, Ulf

AU - Thorsdottir, Inga

AU - Poutanen, Kaisa S

AU - Arner, Peter

AU - Uusitupa, Matti

AU - Kolehmainen, Marjukka

N1 - Funding Information: Funding: This research was funded by the NordForsk Nordic Centre of Excellence in Food, Nutrition and Health project 070014 [SYSDIET (Systems Biology in Controlled Dietary Interventions and Cohort Studies)] and further, the University of Oslo (Norway), Throne Holst Foundation (Norway), Academy of Finland, Swedish Research council, Svenska Diabetesförbundet, SRP Diabetes, Finnish Diabetes Research Foundation, Finnish Foundation for Cardiovascular Research, the Sigrid Juselius Foundation, EVO funding from Kuopio University Hospital (Finland), the Druvan Foundation, Skåne University Hospital, the Heart-Lung Foundation, Diabetesfonden and Foundation Cerealia (Sweden), the Agricultural Productivity Fund, and the Research Fund of the University of Iceland (Iceland). Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/12

Y1 - 2019/12

N2 - A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor necrosis factor (TNF) receptor superfamily member 1A (TNFRSF1A) was down-regulated (p = 0.004), whereas the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit, RELA proto-oncogene, was up-regulated (p = 0.016) in the ND group compared to the CD group. In conclusion, intake of an ND in individuals with the MetS may affect immune function.

AB - A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor necrosis factor (TNF) receptor superfamily member 1A (TNFRSF1A) was down-regulated (p = 0.004), whereas the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit, RELA proto-oncogene, was up-regulated (p = 0.016) in the ND group compared to the CD group. In conclusion, intake of an ND in individuals with the MetS may affect immune function.

UR - http://www.scopus.com/inward/record.url?scp=85076023786&partnerID=8YFLogxK

U2 - 10.3390/nu11122932

DO - 10.3390/nu11122932

M3 - Article

VL - 11

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 12

M1 - 2932

ER -