Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 356-364 |
| Number of pages | 9 |
| Journal | European Journal of Soil Biology |
| Volume | 46 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2010 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Cultured soil archaea
- Halobacterium
- Methanogen
- Enrichment
- Rhizosphere
- Methane
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Anaerobic Eury- and Crenarchaeota inhabit ectomycorrhizas of boreal forest Scots pine. / Bomberg, Malin; Montonen, L. (Corresponding Author); Timonen, S.
In: European Journal of Soil Biology, Vol. 46, No. 6, 2010, p. 356-364.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Anaerobic Eury- and Crenarchaeota inhabit ectomycorrhizas of boreal forest Scots pine
AU - Bomberg, Malin
AU - Montonen, L.
AU - Timonen, S.
PY - 2010
Y1 - 2010
N2 - Members of the euryarchaeotal genera Methanolobus and Halobacterium as well as group 1.1c Crenarchaeota were enriched from ectomycorrhizal samples and cultured under anaerobic conditions. 16S rRNA gene sequences of Methanolobus were obtained in a H2 + CO2 atmosphere and autofluorescent putatively methanogenic microbial cells were detected by epifluorescence microscopy of the anaerobic methane-producing enrichment cultures. Halobacterium and group 1.1c Crenarchaeota grew anaerobically when either H2 or CH4 was added to the atmosphere. Group 1.1c Crenarchaeota were also enriched under aerobic conditions on mineral media, but only when methane or methanol was added as carbon sources. The 16S rRNA gene sequences of 1.1c Crenarchaeota grown under both anaerobic and aerobic conditions were highly similar. Our study demonstrates the growth of group 1.1c Crenarchaeota and Halobacteria derived from non-extreme soil environment in non-saline enrichments under anaerobic conditions. The results suggest that 1.1c Crenarchaeota may play a role in the cycling of C-1 substrates in the boreal forest soil ecosystem.
AB - Members of the euryarchaeotal genera Methanolobus and Halobacterium as well as group 1.1c Crenarchaeota were enriched from ectomycorrhizal samples and cultured under anaerobic conditions. 16S rRNA gene sequences of Methanolobus were obtained in a H2 + CO2 atmosphere and autofluorescent putatively methanogenic microbial cells were detected by epifluorescence microscopy of the anaerobic methane-producing enrichment cultures. Halobacterium and group 1.1c Crenarchaeota grew anaerobically when either H2 or CH4 was added to the atmosphere. Group 1.1c Crenarchaeota were also enriched under aerobic conditions on mineral media, but only when methane or methanol was added as carbon sources. The 16S rRNA gene sequences of 1.1c Crenarchaeota grown under both anaerobic and aerobic conditions were highly similar. Our study demonstrates the growth of group 1.1c Crenarchaeota and Halobacteria derived from non-extreme soil environment in non-saline enrichments under anaerobic conditions. The results suggest that 1.1c Crenarchaeota may play a role in the cycling of C-1 substrates in the boreal forest soil ecosystem.
KW - Cultured soil archaea
KW - Halobacterium
KW - Methanogen
KW - Enrichment
KW - Rhizosphere
KW - Methane
U2 - 10.1016/j.ejsobi.2010.09.002
DO - 10.1016/j.ejsobi.2010.09.002
M3 - Article
VL - 46
SP - 356
EP - 364
JO - European Journal of Soil Biology
JF - European Journal of Soil Biology
SN - 1164-5563
IS - 6
ER -