Anaerobic Eury- and Crenarchaeota inhabit ectomycorrhizas of boreal forest Scots pine

Malin Bomberg, L. Montonen (Corresponding Author), S. Timonen

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Members of the euryarchaeotal genera Methanolobus and Halobacterium as well as group 1.1c Crenarchaeota were enriched from ectomycorrhizal samples and cultured under anaerobic conditions. 16S rRNA gene sequences of Methanolobus were obtained in a H2 + CO2 atmosphere and autofluorescent putatively methanogenic microbial cells were detected by epifluorescence microscopy of the anaerobic methane-producing enrichment cultures. Halobacterium and group 1.1c Crenarchaeota grew anaerobically when either H2 or CH4 was added to the atmosphere. Group 1.1c Crenarchaeota were also enriched under aerobic conditions on mineral media, but only when methane or methanol was added as carbon sources. The 16S rRNA gene sequences of 1.1c Crenarchaeota grown under both anaerobic and aerobic conditions were highly similar. Our study demonstrates the growth of group 1.1c Crenarchaeota and Halobacteria derived from non-extreme soil environment in non-saline enrichments under anaerobic conditions. The results suggest that 1.1c Crenarchaeota may play a role in the cycling of C-1 substrates in the boreal forest soil ecosystem.
Original languageEnglish
Pages (from-to)356-364
Number of pages9
JournalEuropean Journal of Soil Biology
Volume46
Issue number6
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Crenarchaeota
ectomycorrhizae
boreal forests
anoxic conditions
boreal forest
Pinus sylvestris
oxic conditions
Methanosarcinaceae
methane
Halobacterium
anaerobic conditions
soil ecosystem
atmosphere
gene
Methane
aerobic conditions
Atmosphere
rRNA Genes
forest ecosystem
forest soil

Keywords

  • Cultured soil archaea
  • Halobacterium
  • Methanogen
  • Enrichment
  • Rhizosphere
  • Methane

Cite this

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title = "Anaerobic Eury- and Crenarchaeota inhabit ectomycorrhizas of boreal forest Scots pine",
abstract = "Members of the euryarchaeotal genera Methanolobus and Halobacterium as well as group 1.1c Crenarchaeota were enriched from ectomycorrhizal samples and cultured under anaerobic conditions. 16S rRNA gene sequences of Methanolobus were obtained in a H2 + CO2 atmosphere and autofluorescent putatively methanogenic microbial cells were detected by epifluorescence microscopy of the anaerobic methane-producing enrichment cultures. Halobacterium and group 1.1c Crenarchaeota grew anaerobically when either H2 or CH4 was added to the atmosphere. Group 1.1c Crenarchaeota were also enriched under aerobic conditions on mineral media, but only when methane or methanol was added as carbon sources. The 16S rRNA gene sequences of 1.1c Crenarchaeota grown under both anaerobic and aerobic conditions were highly similar. Our study demonstrates the growth of group 1.1c Crenarchaeota and Halobacteria derived from non-extreme soil environment in non-saline enrichments under anaerobic conditions. The results suggest that 1.1c Crenarchaeota may play a role in the cycling of C-1 substrates in the boreal forest soil ecosystem.",
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Anaerobic Eury- and Crenarchaeota inhabit ectomycorrhizas of boreal forest Scots pine. / Bomberg, Malin; Montonen, L. (Corresponding Author); Timonen, S.

In: European Journal of Soil Biology, Vol. 46, No. 6, 2010, p. 356-364.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Anaerobic Eury- and Crenarchaeota inhabit ectomycorrhizas of boreal forest Scots pine

AU - Bomberg, Malin

AU - Montonen, L.

AU - Timonen, S.

PY - 2010

Y1 - 2010

N2 - Members of the euryarchaeotal genera Methanolobus and Halobacterium as well as group 1.1c Crenarchaeota were enriched from ectomycorrhizal samples and cultured under anaerobic conditions. 16S rRNA gene sequences of Methanolobus were obtained in a H2 + CO2 atmosphere and autofluorescent putatively methanogenic microbial cells were detected by epifluorescence microscopy of the anaerobic methane-producing enrichment cultures. Halobacterium and group 1.1c Crenarchaeota grew anaerobically when either H2 or CH4 was added to the atmosphere. Group 1.1c Crenarchaeota were also enriched under aerobic conditions on mineral media, but only when methane or methanol was added as carbon sources. The 16S rRNA gene sequences of 1.1c Crenarchaeota grown under both anaerobic and aerobic conditions were highly similar. Our study demonstrates the growth of group 1.1c Crenarchaeota and Halobacteria derived from non-extreme soil environment in non-saline enrichments under anaerobic conditions. The results suggest that 1.1c Crenarchaeota may play a role in the cycling of C-1 substrates in the boreal forest soil ecosystem.

AB - Members of the euryarchaeotal genera Methanolobus and Halobacterium as well as group 1.1c Crenarchaeota were enriched from ectomycorrhizal samples and cultured under anaerobic conditions. 16S rRNA gene sequences of Methanolobus were obtained in a H2 + CO2 atmosphere and autofluorescent putatively methanogenic microbial cells were detected by epifluorescence microscopy of the anaerobic methane-producing enrichment cultures. Halobacterium and group 1.1c Crenarchaeota grew anaerobically when either H2 or CH4 was added to the atmosphere. Group 1.1c Crenarchaeota were also enriched under aerobic conditions on mineral media, but only when methane or methanol was added as carbon sources. The 16S rRNA gene sequences of 1.1c Crenarchaeota grown under both anaerobic and aerobic conditions were highly similar. Our study demonstrates the growth of group 1.1c Crenarchaeota and Halobacteria derived from non-extreme soil environment in non-saline enrichments under anaerobic conditions. The results suggest that 1.1c Crenarchaeota may play a role in the cycling of C-1 substrates in the boreal forest soil ecosystem.

KW - Cultured soil archaea

KW - Halobacterium

KW - Methanogen

KW - Enrichment

KW - Rhizosphere

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DO - 10.1016/j.ejsobi.2010.09.002

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