Analysis of human gut model metabolites by GCxGC-TOF

Ismo Mattila, Anna-Marja Aura, Sarah Bazzocco, Jarkko Miettinen, Tuulikki Seppänen-Laakso, Matej Oresic

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Dietary phenolic compounds are plant derived secondary metabolites, which can be divided into flavonoids, phenolic acids, stilbenes, tannins and lignans. Catechins can be found as monomers e.g. in tea or as condensed forms, proanthocyanidins (PA), in bilberries, apples, grapes and beverages derived from those fruits. PAs are formed from (+)-catechin and (-)-epicatechin units. Phenolic compounds are ubiquitous metabolites that are metabolised extensively during uptake. It is possible that a major proportion of the metabolites are formed in the colon by microbiota. For example lignans are converted to enterodiol and enterolactone, flavonols are converted to phenylacetic acids and flavanols and flavanones to phenylpropionic acid derivatives. These metabolites, predominantly small phenolic acids, have been identified in human plasma and urine. The residence time of the colonic metabolites is longer than the original structures from plant foods. Epidemiological studies have shown that high concentration of enterolactone is associated to lowered risk of chronic diseases like cancer and cardiovascular disease. Similar evidence for flavanoids and other phenolic compounds is lacking. Identification of diverse flavonoid metabolites is needed. This work aims to compare the in vitro microbial metabolism of (+)-catechin and (-)-epicatechin units using human faecal microbiota as an inoculum to identify their main degradation products. Pure (+)-catechin and (-)-epicatechin were fermented with pooled human faecal microbiota in strictly anaerobic conditions. The sample preparation steps include extraction with ethyl acetate and silylation with MSTFA. GCxGC-TOF analytical method was developed for the analysis of the microbial metabolites. We will describe the method including data processing steps and the preceding sample preparation steps for the analysis of microbial metabolites of (+)-catechin and (-)-epicatechin. The compounds include e.g. hydroxyphenyl propionic and acetic acid derivatives. Financial support of the project STREP-FLAVO (Food-CT-2004-513960) is gratefully acknowledged.
Original languageEnglish
Title of host publicationPlants for Human Health in the Post-Genome Era
Subtitle of host publicationPSE Congress
EditorsAnnemari Kuokka-Ihalainen, Kirsi-Marja Oksman-Caldentey, Heiko Rischer, Anneli Ritala
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages59-59
ISBN (Electronic)978-951-38-6322-7
ISBN (Print)978-951-38-6321-0 978-951-38-6322-7
Publication statusPublished - 2007
EventPSE Congress: Plants for Human Health in the Post-Genome Era - Helsinki, Finland
Duration: 26 Aug 200729 Aug 2007

Publication series

NameVTT Symposium
Number249
ISSN (Print)0357-9387
ISSN (Electronic)1455-0873

Conference

ConferencePSE Congress: Plants for Human Health in the Post-Genome Era
CountryFinland
CityHelsinki
Period26/08/0729/08/07

Fingerprint

Catechin
Metabolites
Lignans
Flavonoids
Phenylacetates
Flavanones
Plasma (human)
Derivatives
Stilbenes
Proanthocyanidins
Flavonols
Beverages
Tannins
Phytochemicals
Fruits
Metabolism
Monomers
Degradation
Acids

Cite this

Mattila, I., Aura, A-M., Bazzocco, S., Miettinen, J., Seppänen-Laakso, T., & Oresic, M. (2007). Analysis of human gut model metabolites by GCxGC-TOF. In A. Kuokka-Ihalainen, K-M. Oksman-Caldentey, H. Rischer, & A. Ritala (Eds.), Plants for Human Health in the Post-Genome Era: PSE Congress (pp. 59-59). [A1] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 249
Mattila, Ismo ; Aura, Anna-Marja ; Bazzocco, Sarah ; Miettinen, Jarkko ; Seppänen-Laakso, Tuulikki ; Oresic, Matej. / Analysis of human gut model metabolites by GCxGC-TOF. Plants for Human Health in the Post-Genome Era: PSE Congress. editor / Annemari Kuokka-Ihalainen ; Kirsi-Marja Oksman-Caldentey ; Heiko Rischer ; Anneli Ritala. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 59-59 (VTT Symposium; No. 249).
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Mattila, I, Aura, A-M, Bazzocco, S, Miettinen, J, Seppänen-Laakso, T & Oresic, M 2007, Analysis of human gut model metabolites by GCxGC-TOF. in A Kuokka-Ihalainen, K-M Oksman-Caldentey, H Rischer & A Ritala (eds), Plants for Human Health in the Post-Genome Era: PSE Congress., A1, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 249, pp. 59-59, PSE Congress: Plants for Human Health in the Post-Genome Era, Helsinki, Finland, 26/08/07.

Analysis of human gut model metabolites by GCxGC-TOF. / Mattila, Ismo; Aura, Anna-Marja; Bazzocco, Sarah; Miettinen, Jarkko; Seppänen-Laakso, Tuulikki; Oresic, Matej.

Plants for Human Health in the Post-Genome Era: PSE Congress. ed. / Annemari Kuokka-Ihalainen; Kirsi-Marja Oksman-Caldentey; Heiko Rischer; Anneli Ritala. Espoo : VTT Technical Research Centre of Finland, 2007. p. 59-59 A1 (VTT Symposium; No. 249).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Analysis of human gut model metabolites by GCxGC-TOF

AU - Mattila, Ismo

AU - Aura, Anna-Marja

AU - Bazzocco, Sarah

AU - Miettinen, Jarkko

AU - Seppänen-Laakso, Tuulikki

AU - Oresic, Matej

PY - 2007

Y1 - 2007

N2 - Dietary phenolic compounds are plant derived secondary metabolites, which can be divided into flavonoids, phenolic acids, stilbenes, tannins and lignans. Catechins can be found as monomers e.g. in tea or as condensed forms, proanthocyanidins (PA), in bilberries, apples, grapes and beverages derived from those fruits. PAs are formed from (+)-catechin and (-)-epicatechin units. Phenolic compounds are ubiquitous metabolites that are metabolised extensively during uptake. It is possible that a major proportion of the metabolites are formed in the colon by microbiota. For example lignans are converted to enterodiol and enterolactone, flavonols are converted to phenylacetic acids and flavanols and flavanones to phenylpropionic acid derivatives. These metabolites, predominantly small phenolic acids, have been identified in human plasma and urine. The residence time of the colonic metabolites is longer than the original structures from plant foods. Epidemiological studies have shown that high concentration of enterolactone is associated to lowered risk of chronic diseases like cancer and cardiovascular disease. Similar evidence for flavanoids and other phenolic compounds is lacking. Identification of diverse flavonoid metabolites is needed. This work aims to compare the in vitro microbial metabolism of (+)-catechin and (-)-epicatechin units using human faecal microbiota as an inoculum to identify their main degradation products. Pure (+)-catechin and (-)-epicatechin were fermented with pooled human faecal microbiota in strictly anaerobic conditions. The sample preparation steps include extraction with ethyl acetate and silylation with MSTFA. GCxGC-TOF analytical method was developed for the analysis of the microbial metabolites. We will describe the method including data processing steps and the preceding sample preparation steps for the analysis of microbial metabolites of (+)-catechin and (-)-epicatechin. The compounds include e.g. hydroxyphenyl propionic and acetic acid derivatives. Financial support of the project STREP-FLAVO (Food-CT-2004-513960) is gratefully acknowledged.

AB - Dietary phenolic compounds are plant derived secondary metabolites, which can be divided into flavonoids, phenolic acids, stilbenes, tannins and lignans. Catechins can be found as monomers e.g. in tea or as condensed forms, proanthocyanidins (PA), in bilberries, apples, grapes and beverages derived from those fruits. PAs are formed from (+)-catechin and (-)-epicatechin units. Phenolic compounds are ubiquitous metabolites that are metabolised extensively during uptake. It is possible that a major proportion of the metabolites are formed in the colon by microbiota. For example lignans are converted to enterodiol and enterolactone, flavonols are converted to phenylacetic acids and flavanols and flavanones to phenylpropionic acid derivatives. These metabolites, predominantly small phenolic acids, have been identified in human plasma and urine. The residence time of the colonic metabolites is longer than the original structures from plant foods. Epidemiological studies have shown that high concentration of enterolactone is associated to lowered risk of chronic diseases like cancer and cardiovascular disease. Similar evidence for flavanoids and other phenolic compounds is lacking. Identification of diverse flavonoid metabolites is needed. This work aims to compare the in vitro microbial metabolism of (+)-catechin and (-)-epicatechin units using human faecal microbiota as an inoculum to identify their main degradation products. Pure (+)-catechin and (-)-epicatechin were fermented with pooled human faecal microbiota in strictly anaerobic conditions. The sample preparation steps include extraction with ethyl acetate and silylation with MSTFA. GCxGC-TOF analytical method was developed for the analysis of the microbial metabolites. We will describe the method including data processing steps and the preceding sample preparation steps for the analysis of microbial metabolites of (+)-catechin and (-)-epicatechin. The compounds include e.g. hydroxyphenyl propionic and acetic acid derivatives. Financial support of the project STREP-FLAVO (Food-CT-2004-513960) is gratefully acknowledged.

M3 - Conference abstract in proceedings

SN - 978-951-38-6321-0 978-951-38-6322-7

T3 - VTT Symposium

SP - 59

EP - 59

BT - Plants for Human Health in the Post-Genome Era

A2 - Kuokka-Ihalainen, Annemari

A2 - Oksman-Caldentey, Kirsi-Marja

A2 - Rischer, Heiko

A2 - Ritala, Anneli

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Mattila I, Aura A-M, Bazzocco S, Miettinen J, Seppänen-Laakso T, Oresic M. Analysis of human gut model metabolites by GCxGC-TOF. In Kuokka-Ihalainen A, Oksman-Caldentey K-M, Rischer H, Ritala A, editors, Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo: VTT Technical Research Centre of Finland. 2007. p. 59-59. A1. (VTT Symposium; No. 249).