Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 42-51 |
| Number of pages | 10 |
| Journal | Carbohydrate Research |
| Volume | 373 |
| DOIs | |
| Publication status | Published - 2013 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- ß-glucosidase
- capillary electrophoresis
- derivatization
- endoglucanase
- ionic liquid
- saccharide
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Analysis of mono- and oligosaccharides in ionic liquid containing matrices. / Wahlström, Ronny (Corresponding Author); Rovio, Stella; Suurnäkki, Anna.
In: Carbohydrate Research, Vol. 373, 2013, p. 42-51.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Analysis of mono- and oligosaccharides in ionic liquid containing matrices
AU - Wahlström, Ronny
AU - Rovio, Stella
AU - Suurnäkki, Anna
PY - 2013
Y1 - 2013
N2 - Ionic liquids (ILs), that is, salts with melting points <100 °C, have recently attracted a lot of attention in biomass processing due to their ability to dissolve lignocellulosics. In this work, we studied how two imidazolium-based, hydrophilic, cellulose dissolving ionic liquids 1,3-dimethylimidazolium dimethylphosphate [DMIM]DMP and 1-ethyl-3-methylimidazolium acetate [EMIM]AcO affect the usually employed analytical methods for mono- and oligosaccharides, typical products from hydrolytic treatments of biomass. HPLC methods were severely hampered by the presence of ILs with loss of separation power and severe baseline problems, making their use for saccharide quantification extremely challenging. Problems in DNS photometric assay and chromatography were also encountered at high ionic liquid concentrations and many capillary electrophoresis (CE) methods did not allow an efficient analysis of saccharides in these matrices. In this paper we describe an optimized CE method with pre-column derivatization for the qualitative and quantitative analysis of mono- and oligosaccharides in sample matrices containing moderate (20–40% (v/v)) concentrations of ILs. The IL content and type in the sample matrix was found to affect both peak shape and quantification parameters. Generally, the presence of high IL concentrations (⩾20% (v/v)) had a dampening effect on the detection of the analytes. IL in lower concentrations of <20% (v/v) was, however, found to improve peak shape and/or separation in some cases. The optimized CE method has good sensitivity in moderate concentrations of the ionic liquids used, with limits of detection of 5 mg/L for cellooligomers up to the size of cellotetraose and 5–20 mg/L for cellopentaose and cellohexaose, depending on the matrix. The method was used for analysing the action of a commercial β-glucosidase in ILs and for analysing saccharides in the IL containing hydrolysates from the hydrolysis of microcrystalline cellulose with Trichoderma reesei endoglucanase Cel5A. According to the results, [DMIM]DMP and [EMIM]AcO] showed clear differences in enzyme inactivation.
AB - Ionic liquids (ILs), that is, salts with melting points <100 °C, have recently attracted a lot of attention in biomass processing due to their ability to dissolve lignocellulosics. In this work, we studied how two imidazolium-based, hydrophilic, cellulose dissolving ionic liquids 1,3-dimethylimidazolium dimethylphosphate [DMIM]DMP and 1-ethyl-3-methylimidazolium acetate [EMIM]AcO affect the usually employed analytical methods for mono- and oligosaccharides, typical products from hydrolytic treatments of biomass. HPLC methods were severely hampered by the presence of ILs with loss of separation power and severe baseline problems, making their use for saccharide quantification extremely challenging. Problems in DNS photometric assay and chromatography were also encountered at high ionic liquid concentrations and many capillary electrophoresis (CE) methods did not allow an efficient analysis of saccharides in these matrices. In this paper we describe an optimized CE method with pre-column derivatization for the qualitative and quantitative analysis of mono- and oligosaccharides in sample matrices containing moderate (20–40% (v/v)) concentrations of ILs. The IL content and type in the sample matrix was found to affect both peak shape and quantification parameters. Generally, the presence of high IL concentrations (⩾20% (v/v)) had a dampening effect on the detection of the analytes. IL in lower concentrations of <20% (v/v) was, however, found to improve peak shape and/or separation in some cases. The optimized CE method has good sensitivity in moderate concentrations of the ionic liquids used, with limits of detection of 5 mg/L for cellooligomers up to the size of cellotetraose and 5–20 mg/L for cellopentaose and cellohexaose, depending on the matrix. The method was used for analysing the action of a commercial β-glucosidase in ILs and for analysing saccharides in the IL containing hydrolysates from the hydrolysis of microcrystalline cellulose with Trichoderma reesei endoglucanase Cel5A. According to the results, [DMIM]DMP and [EMIM]AcO] showed clear differences in enzyme inactivation.
KW - ß-glucosidase
KW - capillary electrophoresis
KW - derivatization
KW - endoglucanase
KW - ionic liquid
KW - saccharide
U2 - 10.1016/j.carres.2012.11.006
DO - 10.1016/j.carres.2012.11.006
M3 - Article
VL - 373
SP - 42
EP - 51
JO - Carbohydrate Research
JF - Carbohydrate Research
SN - 0008-6215
ER -