Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR

Erja Malinen, Teemu Rinttilä, Kajsa Kajander, Jaana Mättö, Anna Kassinen, Lotta Krogius, Maria Saarela, Riitta Korpela, Airi Palva (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

492 Citations (Scopus)

Abstract

OBJECTIVE:

The gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (IBS). In this study, the microbiotas of patients suffering from IBS were compared with a control group devoid of gastrointestinal (GI) symptoms.

METHODS:

Fecal microbiota of patients (n = 27) fulfilling the Rome II criteria for IBS was compared with age- and gender-matched control subjects (n = 22). Fecal samples were obtained at 3 months intervals. Total bacterial DNA was analyzed by 20 quantitative real-time PCR assays covering approximately 300 bacterial species.

RESULTS:

Extensive individual variation was observed in the GI microbiota among both the IBS- and control groups. Sorting of the IBS patients according to the symptom subtypes (diarrhea, constipation, and alternating predominant type) revealed that lower amounts of Lactobacillus spp. were present in the samples of diarrhea predominant IBS patients wheras constipation predominant IBS patients carried increased amounts of Veillonella spp. Average results from three fecal samples suggested differences in the Clostridium coccoides subgroup and Bifidobacterium catenulatum group between IBS patients (n = 21) and controls (n = 15). Of the intestinal pathogens earlier associated with IBS, no indications of Helicobacter spp. or Clostridium difficile were found whereas one case of Campylobacter jejuni was identified by sequencing.

CONCLUSIONS:

With these real-time PCR assays, quantitative alterations in the GI microbiota of IBS patients were found. Increasing microbial DNA sequence information will further allow designing of new real-time PCR assays for a more extensive analysis of intestinal microbes in IBS.

Original languageEnglish
Pages (from-to)373 - 382
Number of pages10
JournalThe American journal of gastroenterology
Volume100
Issue number2
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Fingerprint

Irritable Bowel Syndrome
Microbiota
Real-Time Polymerase Chain Reaction
Constipation
Diarrhea
Veillonella
Helicobacter
Bacterial DNA
Control Groups
Campylobacter jejuni
Bifidobacterium
Clostridium difficile
Clostridium
Lactobacillus
Maintenance

Keywords

  • irritable bowel syndrome
  • IBS
  • gut microbiota
  • gut microflora
  • fecal microbiota
  • gastrointestinal (GI) microbiota

Cite this

Malinen, Erja ; Rinttilä, Teemu ; Kajander, Kajsa ; Mättö, Jaana ; Kassinen, Anna ; Krogius, Lotta ; Saarela, Maria ; Korpela, Riitta ; Palva, Airi. / Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR. In: The American journal of gastroenterology. 2005 ; Vol. 100, No. 2. pp. 373 - 382.
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Malinen, E, Rinttilä, T, Kajander, K, Mättö, J, Kassinen, A, Krogius, L, Saarela, M, Korpela, R & Palva, A 2005, 'Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR', The American journal of gastroenterology, vol. 100, no. 2, pp. 373 - 382. https://doi.org/10.1111/j.1572-0241.2005.40312.x

Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR. / Malinen, Erja; Rinttilä, Teemu; Kajander, Kajsa; Mättö, Jaana; Kassinen, Anna; Krogius, Lotta; Saarela, Maria; Korpela, Riitta; Palva, Airi (Corresponding Author).

In: The American journal of gastroenterology, Vol. 100, No. 2, 2005, p. 373 - 382.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR

AU - Malinen, Erja

AU - Rinttilä, Teemu

AU - Kajander, Kajsa

AU - Mättö, Jaana

AU - Kassinen, Anna

AU - Krogius, Lotta

AU - Saarela, Maria

AU - Korpela, Riitta

AU - Palva, Airi

PY - 2005

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N2 - OBJECTIVE:The gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (IBS). In this study, the microbiotas of patients suffering from IBS were compared with a control group devoid of gastrointestinal (GI) symptoms.METHODS:Fecal microbiota of patients (n = 27) fulfilling the Rome II criteria for IBS was compared with age- and gender-matched control subjects (n = 22). Fecal samples were obtained at 3 months intervals. Total bacterial DNA was analyzed by 20 quantitative real-time PCR assays covering approximately 300 bacterial species.RESULTS:Extensive individual variation was observed in the GI microbiota among both the IBS- and control groups. Sorting of the IBS patients according to the symptom subtypes (diarrhea, constipation, and alternating predominant type) revealed that lower amounts of Lactobacillus spp. were present in the samples of diarrhea predominant IBS patients wheras constipation predominant IBS patients carried increased amounts of Veillonella spp. Average results from three fecal samples suggested differences in the Clostridium coccoides subgroup and Bifidobacterium catenulatum group between IBS patients (n = 21) and controls (n = 15). Of the intestinal pathogens earlier associated with IBS, no indications of Helicobacter spp. or Clostridium difficile were found whereas one case of Campylobacter jejuni was identified by sequencing.CONCLUSIONS:With these real-time PCR assays, quantitative alterations in the GI microbiota of IBS patients were found. Increasing microbial DNA sequence information will further allow designing of new real-time PCR assays for a more extensive analysis of intestinal microbes in IBS.

AB - OBJECTIVE:The gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (IBS). In this study, the microbiotas of patients suffering from IBS were compared with a control group devoid of gastrointestinal (GI) symptoms.METHODS:Fecal microbiota of patients (n = 27) fulfilling the Rome II criteria for IBS was compared with age- and gender-matched control subjects (n = 22). Fecal samples were obtained at 3 months intervals. Total bacterial DNA was analyzed by 20 quantitative real-time PCR assays covering approximately 300 bacterial species.RESULTS:Extensive individual variation was observed in the GI microbiota among both the IBS- and control groups. Sorting of the IBS patients according to the symptom subtypes (diarrhea, constipation, and alternating predominant type) revealed that lower amounts of Lactobacillus spp. were present in the samples of diarrhea predominant IBS patients wheras constipation predominant IBS patients carried increased amounts of Veillonella spp. Average results from three fecal samples suggested differences in the Clostridium coccoides subgroup and Bifidobacterium catenulatum group between IBS patients (n = 21) and controls (n = 15). Of the intestinal pathogens earlier associated with IBS, no indications of Helicobacter spp. or Clostridium difficile were found whereas one case of Campylobacter jejuni was identified by sequencing.CONCLUSIONS:With these real-time PCR assays, quantitative alterations in the GI microbiota of IBS patients were found. Increasing microbial DNA sequence information will further allow designing of new real-time PCR assays for a more extensive analysis of intestinal microbes in IBS.

KW - irritable bowel syndrome

KW - IBS

KW - gut microbiota

KW - gut microflora

KW - fecal microbiota

KW - gastrointestinal (GI) microbiota

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M3 - Article

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