Abstract
Theβ-exotoxin fromBacillus thuringensis is a well known insecticide. The bioassay usually employed for its analysis is time-consuming and subject to many disturbances. The previously reported HPLC analysis has been shown to give erroneous results in some cases.
A new analysis system has how been designed. The system is based on separation ofβ-exotoxin from the culture broth, and from various preparations containingβ-exotoxin by ion-pairing. For sample preparation in more complex matrices an method employing solid phase extraction was developed.
In order to obtain a standard for quantitative analysis a preparative method for the isolation ofβ-exotoxin from concentrated culture broth after successive precipitations was also developed.
A new analysis system has how been designed. The system is based on separation ofβ-exotoxin from the culture broth, and from various preparations containingβ-exotoxin by ion-pairing. For sample preparation in more complex matrices an method employing solid phase extraction was developed.
In order to obtain a standard for quantitative analysis a preparative method for the isolation ofβ-exotoxin from concentrated culture broth after successive precipitations was also developed.
| Original language | English |
|---|---|
| Pages (from-to) | 745-747 |
| Journal | Chromatographia |
| Volume | 24 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1987 |
| MoE publication type | A1 Journal article-refereed |