Analytical and preparative methods for β-exotoxin fromBacillus Thuringensis

Tapani Suortti, Lasse Ylinen, Pirkko Kärppä

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Theβ-exotoxin fromBacillus thuringensis is a well known insecticide. The bioassay usually employed for its analysis is time-consuming and subject to many disturbances. The previously reported HPLC analysis has been shown to give erroneous results in some cases.

A new analysis system has how been designed. The system is based on separation ofβ-exotoxin from the culture broth, and from various preparations containingβ-exotoxin by ion-pairing. For sample preparation in more complex matrices an method employing solid phase extraction was developed.

In order to obtain a standard for quantitative analysis a preparative method for the isolation ofβ-exotoxin from concentrated culture broth after successive precipitations was also developed.
Original languageEnglish
Pages (from-to)745-747
JournalChromatographia
Volume24
Issue number1
DOIs
Publication statusPublished - 1987
MoE publication typeA1 Journal article-refereed

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