Analytical and preparative methods for β-exotoxin fromBacillus Thuringensis

Tapani Suortti, Lasse Ylinen, Pirkko Kärppä

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2 Citations (Scopus)

Abstract

Theβ-exotoxin fromBacillus thuringensis is a well known insecticide. The bioassay usually employed for its analysis is time-consuming and subject to many disturbances. The previously reported HPLC analysis has been shown to give erroneous results in some cases.

A new analysis system has how been designed. The system is based on separation ofβ-exotoxin from the culture broth, and from various preparations containingβ-exotoxin by ion-pairing. For sample preparation in more complex matrices an method employing solid phase extraction was developed.

In order to obtain a standard for quantitative analysis a preparative method for the isolation ofβ-exotoxin from concentrated culture broth after successive precipitations was also developed.
Original languageEnglish
Pages (from-to)745-747
JournalChromatographia
Volume24
Issue number1
DOIs
Publication statusPublished - 1987
MoE publication typeA1 Journal article-refereed

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Exotoxins
Bioassay
Solid Phase Extraction
Insecticides
Biological Assay
High Pressure Liquid Chromatography
Ions
Chemical analysis

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Suortti, Tapani ; Ylinen, Lasse ; Kärppä, Pirkko. / Analytical and preparative methods for β-exotoxin fromBacillus Thuringensis. In: Chromatographia. 1987 ; Vol. 24, No. 1. pp. 745-747.
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Analytical and preparative methods for β-exotoxin fromBacillus Thuringensis. / Suortti, Tapani; Ylinen, Lasse; Kärppä, Pirkko.

In: Chromatographia, Vol. 24, No. 1, 1987, p. 745-747.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Analytical and preparative methods for β-exotoxin fromBacillus Thuringensis

AU - Suortti, Tapani

AU - Ylinen, Lasse

AU - Kärppä, Pirkko

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N2 - Theβ-exotoxin fromBacillus thuringensis is a well known insecticide. The bioassay usually employed for its analysis is time-consuming and subject to many disturbances. The previously reported HPLC analysis has been shown to give erroneous results in some cases.A new analysis system has how been designed. The system is based on separation ofβ-exotoxin from the culture broth, and from various preparations containingβ-exotoxin by ion-pairing. For sample preparation in more complex matrices an method employing solid phase extraction was developed.In order to obtain a standard for quantitative analysis a preparative method for the isolation ofβ-exotoxin from concentrated culture broth after successive precipitations was also developed.

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