Antifungal activities of two Lactobacillus plantarum strains against Fusarium moulds in vitro and in malting of barley

Arja Laitila (Corresponding Author), Hanna-Leena Alakomi, Laura Raaska, Tiina Mattila-Sandholm, Auli Haikara

Research output: Contribution to journalArticleScientificpeer-review

102 Citations (Scopus)

Abstract

Aims: The Lactobacillus plantarum strains VTT E‐78076 (E76) and VTT E‐79098 (E98) were studied for their antifungal potential against Fusarium species.
Methods and Results:In vitro screening with automated turbidometry as well as direct and indirect impedimetric methods clearly showed Lact. plantarum cell‐free extracts to be effective against Fusarium species including Fusarium avenaceum, F. culmorum, F. graminearum and F.oxysporum. However, great variation in growth inhibition was observed between different Fusarium species and even between strains. The antifungal potential of Lact. plantarum E76 culture, including cells and spent medium, was also examined in laboratory‐scale malting with naturally contaminated two‐rowed barley from the crops of 1990–96. The growth of the indigenous Fusarium flora was restricted by the addition of Lact. plantarum E76 to the steeping water. However, the antifungal effect was greatly dependent on the contamination level and the fungal species/strains present on barley in different years.
Conclusions:Lactobacillus plantarum strains E76 and E98 had a fungistatic effect against different plant pathogenic, toxigenic and gushing‐active Fusarium fungi.
Significance and Impact of the Study: The present study indicates that Lact. plantarum strains with known and selected characteristics could be used as a natural, food‐grade biocontrol agent for management of problems caused by Fusarium fungi during germination of cereals.
Original languageEnglish
Pages (from-to)566-576
JournalJournal of Applied Microbiology
Volume93
Issue number4
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

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Lactobacillus plantarum
Fusarium
Hordeum
Fungi
Growth
Germination
In Vitro Techniques
Cell Culture Techniques
Water

Keywords

  • Lactobacillus plantarum
  • Lactobacillus
  • Fusarium
  • fungi
  • cereals
  • biocontrol

Cite this

Laitila, Arja ; Alakomi, Hanna-Leena ; Raaska, Laura ; Mattila-Sandholm, Tiina ; Haikara, Auli. / Antifungal activities of two Lactobacillus plantarum strains against Fusarium moulds in vitro and in malting of barley. In: Journal of Applied Microbiology. 2002 ; Vol. 93, No. 4. pp. 566-576.
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abstract = "Aims: The Lactobacillus plantarum strains VTT E‐78076 (E76) and VTT E‐79098 (E98) were studied for their antifungal potential against Fusarium species. Methods and Results:In vitro screening with automated turbidometry as well as direct and indirect impedimetric methods clearly showed Lact. plantarum cell‐free extracts to be effective against Fusarium species including Fusarium avenaceum, F. culmorum, F. graminearum and F.oxysporum. However, great variation in growth inhibition was observed between different Fusarium species and even between strains. The antifungal potential of Lact. plantarum E76 culture, including cells and spent medium, was also examined in laboratory‐scale malting with naturally contaminated two‐rowed barley from the crops of 1990–96. The growth of the indigenous Fusarium flora was restricted by the addition of Lact. plantarum E76 to the steeping water. However, the antifungal effect was greatly dependent on the contamination level and the fungal species/strains present on barley in different years. Conclusions:Lactobacillus plantarum strains E76 and E98 had a fungistatic effect against different plant pathogenic, toxigenic and gushing‐active Fusarium fungi. Significance and Impact of the Study: The present study indicates that Lact. plantarum strains with known and selected characteristics could be used as a natural, food‐grade biocontrol agent for management of problems caused by Fusarium fungi during germination of cereals.",
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author = "Arja Laitila and Hanna-Leena Alakomi and Laura Raaska and Tiina Mattila-Sandholm and Auli Haikara",
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doi = "10.1046/j.1365-2672.2002.01731.x",
language = "English",
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pages = "566--576",
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Antifungal activities of two Lactobacillus plantarum strains against Fusarium moulds in vitro and in malting of barley. / Laitila, Arja (Corresponding Author); Alakomi, Hanna-Leena; Raaska, Laura; Mattila-Sandholm, Tiina; Haikara, Auli.

In: Journal of Applied Microbiology, Vol. 93, No. 4, 2002, p. 566-576.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Antifungal activities of two Lactobacillus plantarum strains against Fusarium moulds in vitro and in malting of barley

AU - Laitila, Arja

AU - Alakomi, Hanna-Leena

AU - Raaska, Laura

AU - Mattila-Sandholm, Tiina

AU - Haikara, Auli

PY - 2002

Y1 - 2002

N2 - Aims: The Lactobacillus plantarum strains VTT E‐78076 (E76) and VTT E‐79098 (E98) were studied for their antifungal potential against Fusarium species. Methods and Results:In vitro screening with automated turbidometry as well as direct and indirect impedimetric methods clearly showed Lact. plantarum cell‐free extracts to be effective against Fusarium species including Fusarium avenaceum, F. culmorum, F. graminearum and F.oxysporum. However, great variation in growth inhibition was observed between different Fusarium species and even between strains. The antifungal potential of Lact. plantarum E76 culture, including cells and spent medium, was also examined in laboratory‐scale malting with naturally contaminated two‐rowed barley from the crops of 1990–96. The growth of the indigenous Fusarium flora was restricted by the addition of Lact. plantarum E76 to the steeping water. However, the antifungal effect was greatly dependent on the contamination level and the fungal species/strains present on barley in different years. Conclusions:Lactobacillus plantarum strains E76 and E98 had a fungistatic effect against different plant pathogenic, toxigenic and gushing‐active Fusarium fungi. Significance and Impact of the Study: The present study indicates that Lact. plantarum strains with known and selected characteristics could be used as a natural, food‐grade biocontrol agent for management of problems caused by Fusarium fungi during germination of cereals.

AB - Aims: The Lactobacillus plantarum strains VTT E‐78076 (E76) and VTT E‐79098 (E98) were studied for their antifungal potential against Fusarium species. Methods and Results:In vitro screening with automated turbidometry as well as direct and indirect impedimetric methods clearly showed Lact. plantarum cell‐free extracts to be effective against Fusarium species including Fusarium avenaceum, F. culmorum, F. graminearum and F.oxysporum. However, great variation in growth inhibition was observed between different Fusarium species and even between strains. The antifungal potential of Lact. plantarum E76 culture, including cells and spent medium, was also examined in laboratory‐scale malting with naturally contaminated two‐rowed barley from the crops of 1990–96. The growth of the indigenous Fusarium flora was restricted by the addition of Lact. plantarum E76 to the steeping water. However, the antifungal effect was greatly dependent on the contamination level and the fungal species/strains present on barley in different years. Conclusions:Lactobacillus plantarum strains E76 and E98 had a fungistatic effect against different plant pathogenic, toxigenic and gushing‐active Fusarium fungi. Significance and Impact of the Study: The present study indicates that Lact. plantarum strains with known and selected characteristics could be used as a natural, food‐grade biocontrol agent for management of problems caused by Fusarium fungi during germination of cereals.

KW - Lactobacillus plantarum

KW - Lactobacillus

KW - Fusarium

KW - fungi

KW - cereals

KW - biocontrol

U2 - 10.1046/j.1365-2672.2002.01731.x

DO - 10.1046/j.1365-2672.2002.01731.x

M3 - Article

VL - 93

SP - 566

EP - 576

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 4

ER -