Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 584-593 |
| Journal | Plant Cell Reports |
| Volume | 22 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2004 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- stilbenes
- pinosylvin
- resveratrol
- decay fungi
- transformation
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Antifungal activity of stilbenes in in vitro bioassays and in transgenic Populus expressing a gene encoding pinosylvin synthase. / Seppänen, S.K. (Corresponding Author); Syrjälä, L.; von Weissenberg, K.; Teeri, T.H.; Paajanen, Leena; Pappinen, A.
In: Plant Cell Reports, Vol. 22, No. 8, 2004, p. 584-593.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Antifungal activity of stilbenes in in vitro bioassays and in transgenic Populus expressing a gene encoding pinosylvin synthase
AU - Seppänen, S.K.
AU - Syrjälä, L.
AU - von Weissenberg, K.
AU - Teeri, T.H.
AU - Paajanen, Leena
AU - Pappinen, A.
PY - 2004
Y1 - 2004
N2 - The effect of two stilbene compounds, pinosylvin and resveratrol, on the growth of several fungi was evaluated in plate tests. Wood decay tests were carried out with birch and aspen samples impregnated with the two stilbenes. In plate experiments, resveratrol had an enhancing effect on growth at concentrations where pinosylvin was already enough to prevent the growth of most fungi studied. Pinosylvin impregnated at 0.2% (w/w) concentration significantly reduced the decay caused by all fungi except Phellinus tremulae. In contrast, a resveratrol content of 0.8%, did not protect the wood from decay. A pinosylvin-synthase-encoding gene from Pinus sylvestris was transferred into aspen (Populus tremula) and two hybrid aspen clones (Populus tremula×tremuloides) by Agrobacterium tumefaciens-mediated transformation. Transgenic plants accumulated pinosylvin synthase-specific mRNA and showed stilbene synthase enzyme activity in vitro. Transgenic aspen line H4 showed increased resistance to Phellinus tremulae, while two hybrid aspen transformants decayed faster than the control trees. However, we were unable to detect the accumulation of stilbenes in the transgenic plantlets.
AB - The effect of two stilbene compounds, pinosylvin and resveratrol, on the growth of several fungi was evaluated in plate tests. Wood decay tests were carried out with birch and aspen samples impregnated with the two stilbenes. In plate experiments, resveratrol had an enhancing effect on growth at concentrations where pinosylvin was already enough to prevent the growth of most fungi studied. Pinosylvin impregnated at 0.2% (w/w) concentration significantly reduced the decay caused by all fungi except Phellinus tremulae. In contrast, a resveratrol content of 0.8%, did not protect the wood from decay. A pinosylvin-synthase-encoding gene from Pinus sylvestris was transferred into aspen (Populus tremula) and two hybrid aspen clones (Populus tremula×tremuloides) by Agrobacterium tumefaciens-mediated transformation. Transgenic plants accumulated pinosylvin synthase-specific mRNA and showed stilbene synthase enzyme activity in vitro. Transgenic aspen line H4 showed increased resistance to Phellinus tremulae, while two hybrid aspen transformants decayed faster than the control trees. However, we were unable to detect the accumulation of stilbenes in the transgenic plantlets.
KW - stilbenes
KW - pinosylvin
KW - resveratrol
KW - decay fungi
KW - transformation
U2 - 10.1007/s00299-003-0728-0
DO - 10.1007/s00299-003-0728-0
M3 - Article
VL - 22
SP - 584
EP - 593
JO - Plant Cell Reports
JF - Plant Cell Reports
SN - 0721-7714
IS - 8
ER -