Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films

Jari Vartiainen (Corresponding Author), Marjaana Rättö, Sabine Paulussen

    Research output: Contribution to journalArticleScientificpeer-review

    79 Citations (Scopus)

    Abstract

    Antimicrobial enzyme, glucose oxidase (GOX), was covalently immobilized onto amino‐ and carboxyl‐plasma‐activated biorientated polypropylene films (BOPP) via glutaraldehyde and carbodiimide chemistries. N2‐plasma + NH3 and N2‐plasma + CO2 treatments were utilized to create amino (1.1 nmol/cm2) and carboxyl (0.9 nmol/cm2) groups densities onto the surface of BOPP films. GOX‐immobilized onto amino‐activated BOPP films using 2.5% glutaraldehyde produced higher enzymatic activities than GOX‐immobilized by 0.4% carbodiimide. Further immobilizations were carried out with glutaraldehyde as the coupling agent at temperatures of 4–75°C at pH 5.6 and 7.2. 10 s treatment was sufficient to immobilize GOX at high temperatures in both pH conditions, producing enzymatically active films which remained active over 30 days of storage. GOX covalently immobilized onto BOPP films completely inhibited the growth of Escherichia coli and substantially inhibited the growth of Bacillus subtilis; thus, they may have great potential to be exploited in various antimicrobial packaging film applications.
    Original languageEnglish
    Pages (from-to)243 - 251
    Number of pages9
    JournalPackaging Technology and Science
    Volume18
    Issue number5
    DOIs
    Publication statusPublished - 2005
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Glucose Oxidase
    Glucose oxidase
    Polypropylenes
    Plasmas
    Glutaral
    Carbodiimides
    Coupling agents
    Bacilli
    Escherichia coli
    Packaging
    Enzymes
    Temperature

    Keywords

    • plastics
    • enzymes
    • antimicrobial properties
    • food packaging

    Cite this

    Vartiainen, Jari ; Rättö, Marjaana ; Paulussen, Sabine. / Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films. In: Packaging Technology and Science. 2005 ; Vol. 18, No. 5. pp. 243 - 251.
    @article{d0ff20d721804317b6cf2971e5e22978,
    title = "Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films",
    abstract = "Antimicrobial enzyme, glucose oxidase (GOX), was covalently immobilized onto amino‐ and carboxyl‐plasma‐activated biorientated polypropylene films (BOPP) via glutaraldehyde and carbodiimide chemistries. N2‐plasma + NH3 and N2‐plasma + CO2 treatments were utilized to create amino (1.1 nmol/cm2) and carboxyl (0.9 nmol/cm2) groups densities onto the surface of BOPP films. GOX‐immobilized onto amino‐activated BOPP films using 2.5{\%} glutaraldehyde produced higher enzymatic activities than GOX‐immobilized by 0.4{\%} carbodiimide. Further immobilizations were carried out with glutaraldehyde as the coupling agent at temperatures of 4–75°C at pH 5.6 and 7.2. 10 s treatment was sufficient to immobilize GOX at high temperatures in both pH conditions, producing enzymatically active films which remained active over 30 days of storage. GOX covalently immobilized onto BOPP films completely inhibited the growth of Escherichia coli and substantially inhibited the growth of Bacillus subtilis; thus, they may have great potential to be exploited in various antimicrobial packaging film applications.",
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    author = "Jari Vartiainen and Marjaana R{\"a}tt{\"o} and Sabine Paulussen",
    year = "2005",
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    Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films. / Vartiainen, Jari (Corresponding Author); Rättö, Marjaana; Paulussen, Sabine.

    In: Packaging Technology and Science, Vol. 18, No. 5, 2005, p. 243 - 251.

    Research output: Contribution to journalArticleScientificpeer-review

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    AU - Vartiainen, Jari

    AU - Rättö, Marjaana

    AU - Paulussen, Sabine

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    N2 - Antimicrobial enzyme, glucose oxidase (GOX), was covalently immobilized onto amino‐ and carboxyl‐plasma‐activated biorientated polypropylene films (BOPP) via glutaraldehyde and carbodiimide chemistries. N2‐plasma + NH3 and N2‐plasma + CO2 treatments were utilized to create amino (1.1 nmol/cm2) and carboxyl (0.9 nmol/cm2) groups densities onto the surface of BOPP films. GOX‐immobilized onto amino‐activated BOPP films using 2.5% glutaraldehyde produced higher enzymatic activities than GOX‐immobilized by 0.4% carbodiimide. Further immobilizations were carried out with glutaraldehyde as the coupling agent at temperatures of 4–75°C at pH 5.6 and 7.2. 10 s treatment was sufficient to immobilize GOX at high temperatures in both pH conditions, producing enzymatically active films which remained active over 30 days of storage. GOX covalently immobilized onto BOPP films completely inhibited the growth of Escherichia coli and substantially inhibited the growth of Bacillus subtilis; thus, they may have great potential to be exploited in various antimicrobial packaging film applications.

    AB - Antimicrobial enzyme, glucose oxidase (GOX), was covalently immobilized onto amino‐ and carboxyl‐plasma‐activated biorientated polypropylene films (BOPP) via glutaraldehyde and carbodiimide chemistries. N2‐plasma + NH3 and N2‐plasma + CO2 treatments were utilized to create amino (1.1 nmol/cm2) and carboxyl (0.9 nmol/cm2) groups densities onto the surface of BOPP films. GOX‐immobilized onto amino‐activated BOPP films using 2.5% glutaraldehyde produced higher enzymatic activities than GOX‐immobilized by 0.4% carbodiimide. Further immobilizations were carried out with glutaraldehyde as the coupling agent at temperatures of 4–75°C at pH 5.6 and 7.2. 10 s treatment was sufficient to immobilize GOX at high temperatures in both pH conditions, producing enzymatically active films which remained active over 30 days of storage. GOX covalently immobilized onto BOPP films completely inhibited the growth of Escherichia coli and substantially inhibited the growth of Bacillus subtilis; thus, they may have great potential to be exploited in various antimicrobial packaging film applications.

    KW - plastics

    KW - enzymes

    KW - antimicrobial properties

    KW - food packaging

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