Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films

Jari Vartiainen (Corresponding Author), Marjaana Rättö, Sabine Paulussen

Research output: Contribution to journalArticleScientificpeer-review

79 Citations (Scopus)

Abstract

Antimicrobial enzyme, glucose oxidase (GOX), was covalently immobilized onto amino‐ and carboxyl‐plasma‐activated biorientated polypropylene films (BOPP) via glutaraldehyde and carbodiimide chemistries. N2‐plasma + NH3 and N2‐plasma + CO2 treatments were utilized to create amino (1.1 nmol/cm2) and carboxyl (0.9 nmol/cm2) groups densities onto the surface of BOPP films. GOX‐immobilized onto amino‐activated BOPP films using 2.5% glutaraldehyde produced higher enzymatic activities than GOX‐immobilized by 0.4% carbodiimide. Further immobilizations were carried out with glutaraldehyde as the coupling agent at temperatures of 4–75°C at pH 5.6 and 7.2. 10 s treatment was sufficient to immobilize GOX at high temperatures in both pH conditions, producing enzymatically active films which remained active over 30 days of storage. GOX covalently immobilized onto BOPP films completely inhibited the growth of Escherichia coli and substantially inhibited the growth of Bacillus subtilis; thus, they may have great potential to be exploited in various antimicrobial packaging film applications.
Original languageEnglish
Pages (from-to)243 - 251
Number of pages9
JournalPackaging Technology and Science
Volume18
Issue number5
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Fingerprint

Glucose Oxidase
Glucose oxidase
Polypropylenes
Plasmas
Glutaral
Carbodiimides
Coupling agents
Bacilli
Escherichia coli
Packaging
Enzymes
Temperature

Keywords

  • plastics
  • enzymes
  • antimicrobial properties
  • food packaging

Cite this

Vartiainen, Jari ; Rättö, Marjaana ; Paulussen, Sabine. / Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films. In: Packaging Technology and Science. 2005 ; Vol. 18, No. 5. pp. 243 - 251.
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title = "Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films",
abstract = "Antimicrobial enzyme, glucose oxidase (GOX), was covalently immobilized onto amino‐ and carboxyl‐plasma‐activated biorientated polypropylene films (BOPP) via glutaraldehyde and carbodiimide chemistries. N2‐plasma + NH3 and N2‐plasma + CO2 treatments were utilized to create amino (1.1 nmol/cm2) and carboxyl (0.9 nmol/cm2) groups densities onto the surface of BOPP films. GOX‐immobilized onto amino‐activated BOPP films using 2.5{\%} glutaraldehyde produced higher enzymatic activities than GOX‐immobilized by 0.4{\%} carbodiimide. Further immobilizations were carried out with glutaraldehyde as the coupling agent at temperatures of 4–75°C at pH 5.6 and 7.2. 10 s treatment was sufficient to immobilize GOX at high temperatures in both pH conditions, producing enzymatically active films which remained active over 30 days of storage. GOX covalently immobilized onto BOPP films completely inhibited the growth of Escherichia coli and substantially inhibited the growth of Bacillus subtilis; thus, they may have great potential to be exploited in various antimicrobial packaging film applications.",
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Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films. / Vartiainen, Jari (Corresponding Author); Rättö, Marjaana; Paulussen, Sabine.

In: Packaging Technology and Science, Vol. 18, No. 5, 2005, p. 243 - 251.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Antimicrobial activity of glucose oxidase-immobilized plasma-activated polypropylene films

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AU - Rättö, Marjaana

AU - Paulussen, Sabine

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AB - Antimicrobial enzyme, glucose oxidase (GOX), was covalently immobilized onto amino‐ and carboxyl‐plasma‐activated biorientated polypropylene films (BOPP) via glutaraldehyde and carbodiimide chemistries. N2‐plasma + NH3 and N2‐plasma + CO2 treatments were utilized to create amino (1.1 nmol/cm2) and carboxyl (0.9 nmol/cm2) groups densities onto the surface of BOPP films. GOX‐immobilized onto amino‐activated BOPP films using 2.5% glutaraldehyde produced higher enzymatic activities than GOX‐immobilized by 0.4% carbodiimide. Further immobilizations were carried out with glutaraldehyde as the coupling agent at temperatures of 4–75°C at pH 5.6 and 7.2. 10 s treatment was sufficient to immobilize GOX at high temperatures in both pH conditions, producing enzymatically active films which remained active over 30 days of storage. GOX covalently immobilized onto BOPP films completely inhibited the growth of Escherichia coli and substantially inhibited the growth of Bacillus subtilis; thus, they may have great potential to be exploited in various antimicrobial packaging film applications.

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