Antimicrobial susceptibility of Lactobacillus rhamnosus

J.-M. Korhonen (Corresponding Author), A.H.A.M. Van Hoek, Maria Saarela, G. Huys, L. Tosi, S. Mayrhofer, Atte von Wright

    Research output: Contribution to journalArticleScientificpeer-review

    19 Citations (Scopus)

    Abstract

    We aimed to determine the minimum inhibitory concentrations (MICs) of Lactobacillus rhamnosus (n=75) strains, to study their antibiotic resistance genes with microarray, and to assess the microbiological cut-off values of tested antimicrobial agents. L. rhamnosus strains were tested with agar dilution, broth microdilution and Etest methods for ampicillin, clindamycin, erythromycin, gentamicin, streptomycin, and tetracycline using specific LSM medium. Most of the L. rhamnosus strains were found phenotypically susceptible to all six antibiotics tested. Four of the strains were phenotypically multiresistant, three strains to clindamycin, erythromycin and streptomycin and one strain to streptomycin and tetracycline. Some of the resistant (n=8) and susceptible (n=5) strains were further studied with a microarray method to reveal the antibiotic resistance genes behind the phenotypic resistances. From our experience, we recommend that microbiological cut-off values should be proposed according to the method used.
    Original languageEnglish
    Pages (from-to)75-80
    Number of pages6
    JournalBeneficial Microbes
    Volume1
    Issue number1
    DOIs
    Publication statusPublished - 2010
    MoE publication typeA1 Journal article-refereed

    Keywords

    • minimum inhibitory concentration (MIC)
    • agar dilution
    • broth microdilution
    • Etest
    • microarray

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