Antimicrobial susceptibility of Lactobacillus rhamnosus

J.-M. Korhonen (Corresponding Author), A.H.A.M. Van Hoek, Maria Saarela, G. Huys, L. Tosi, S. Mayrhofer, Atte von Wright

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

We aimed to determine the minimum inhibitory concentrations (MICs) of Lactobacillus rhamnosus (n=75) strains, to study their antibiotic resistance genes with microarray, and to assess the microbiological cut-off values of tested antimicrobial agents. L. rhamnosus strains were tested with agar dilution, broth microdilution and Etest methods for ampicillin, clindamycin, erythromycin, gentamicin, streptomycin, and tetracycline using specific LSM medium. Most of the L. rhamnosus strains were found phenotypically susceptible to all six antibiotics tested. Four of the strains were phenotypically multiresistant, three strains to clindamycin, erythromycin and streptomycin and one strain to streptomycin and tetracycline. Some of the resistant (n=8) and susceptible (n=5) strains were further studied with a microarray method to reveal the antibiotic resistance genes behind the phenotypic resistances. From our experience, we recommend that microbiological cut-off values should be proposed according to the method used.
Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalBeneficial Microbes
Volume1
Issue number1
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Lactobacillus rhamnosus
Streptomycin
Clindamycin
Erythromycin
Microbial Drug Resistance
Tetracycline
Disk Diffusion Antimicrobial Tests
Microbial Sensitivity Tests
Ampicillin
Gentamicins
Genes
Agar
Anti-Bacterial Agents

Keywords

  • minimum inhibitory concentration (MIC)
  • agar dilution
  • broth microdilution
  • Etest
  • microarray

Cite this

Korhonen, J-M., Van Hoek, A. H. A. M., Saarela, M., Huys, G., Tosi, L., Mayrhofer, S., & von Wright, A. (2010). Antimicrobial susceptibility of Lactobacillus rhamnosus. Beneficial Microbes, 1(1), 75-80. https://doi.org/10.3920/BM2009.0002
Korhonen, J.-M. ; Van Hoek, A.H.A.M. ; Saarela, Maria ; Huys, G. ; Tosi, L. ; Mayrhofer, S. ; von Wright, Atte. / Antimicrobial susceptibility of Lactobacillus rhamnosus. In: Beneficial Microbes. 2010 ; Vol. 1, No. 1. pp. 75-80.
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Korhonen, J-M, Van Hoek, AHAM, Saarela, M, Huys, G, Tosi, L, Mayrhofer, S & von Wright, A 2010, 'Antimicrobial susceptibility of Lactobacillus rhamnosus', Beneficial Microbes, vol. 1, no. 1, pp. 75-80. https://doi.org/10.3920/BM2009.0002

Antimicrobial susceptibility of Lactobacillus rhamnosus. / Korhonen, J.-M. (Corresponding Author); Van Hoek, A.H.A.M.; Saarela, Maria; Huys, G.; Tosi, L.; Mayrhofer, S.; von Wright, Atte.

In: Beneficial Microbes, Vol. 1, No. 1, 2010, p. 75-80.

Research output: Contribution to journalArticleScientificpeer-review

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Korhonen J-M, Van Hoek AHAM, Saarela M, Huys G, Tosi L, Mayrhofer S et al. Antimicrobial susceptibility of Lactobacillus rhamnosus. Beneficial Microbes. 2010;1(1):75-80. https://doi.org/10.3920/BM2009.0002