Abstract
We aimed to determine the minimum inhibitory concentrations (MICs) of Lactobacillus rhamnosus (n=75) strains,
to study their antibiotic resistance genes with microarray, and to assess the microbiological cut-off values of tested
antimicrobial agents. L. rhamnosus strains were tested with agar dilution, broth microdilution and Etest methods
for ampicillin, clindamycin, erythromycin, gentamicin, streptomycin, and tetracycline using specific LSM medium.
Most of the L. rhamnosus strains were found phenotypically susceptible to all six antibiotics tested. Four of the strains
were phenotypically multiresistant, three strains to clindamycin, erythromycin and streptomycin and one strain
to streptomycin and tetracycline. Some of the resistant (n=8) and susceptible (n=5) strains were further studied
with a microarray method to reveal the antibiotic resistance genes behind the phenotypic resistances. From our
experience, we recommend that microbiological cut-off values should be proposed according to the method used.
| Original language | English |
|---|---|
| Pages (from-to) | 75-80 |
| Journal | Beneficial Microbes |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- minimum inhibitory concentration (MIC)
- agar dilution
- broth microdilution
- Etest
- microarray