apaH polymorphism in clinical Actinobacillus actinomycetemcomitans isolates

Maria Saarela, Sirkka Asikainen, Satu Alaluusua, Paula Fives-Taylor

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

In the present study theapaHpolymorphism in clinicalA. actinomycetemcomitansisolates were investigated in relation to their serotype and periodontal status of the donor subjects. The material included 122A. actinomycetemcomitansisolates representing serotypes a–e from 103 periodontally healthy and diseased subjects.
TheapaHpolymorphism was investigated by both restriction analysis of the specific PCR amplification product and also by sequencing of PCR amplification products from selected clinical isolates.
AnapaHspecific PCR amplification product was obtained from all isolates but the restriction patterns of the amplification products varied. Serotype c and genogroup 2 within serotype e formed genetically distinct groups, whereas isolates of serotype a, b, d and genogroup 1 within serotype e could not be separated from each other based on theapaHrestriction analysis.
No relation between the restriction pattern ofapaHand the periodontal status of the individuals was detected.
These results indicate that serotype c isolates form a uniformly distinct group withinA. actinomycetemcomitansand that a subpopulation of serotype e isolates clearly diverge from all otherA. actinomycetemcomitansisolates.
Original languageEnglish
Pages (from-to)139-144
JournalAnaerobe
Volume4
Issue number3
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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Aggregatibacter actinomycetemcomitans
Polymerase Chain Reaction
Genotype
Serogroup
Healthy Volunteers
Tissue Donors

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Saarela, M., Asikainen, S., Alaluusua, S., & Fives-Taylor, P. (1998). apaH polymorphism in clinical Actinobacillus actinomycetemcomitans isolates. Anaerobe, 4(3), 139-144. https://doi.org/10.1006/anae.1998.0158
Saarela, Maria ; Asikainen, Sirkka ; Alaluusua, Satu ; Fives-Taylor, Paula. / apaH polymorphism in clinical Actinobacillus actinomycetemcomitans isolates. In: Anaerobe. 1998 ; Vol. 4, No. 3. pp. 139-144.
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Saarela, M, Asikainen, S, Alaluusua, S & Fives-Taylor, P 1998, 'apaH polymorphism in clinical Actinobacillus actinomycetemcomitans isolates', Anaerobe, vol. 4, no. 3, pp. 139-144. https://doi.org/10.1006/anae.1998.0158

apaH polymorphism in clinical Actinobacillus actinomycetemcomitans isolates. / Saarela, Maria; Asikainen, Sirkka; Alaluusua, Satu; Fives-Taylor, Paula.

In: Anaerobe, Vol. 4, No. 3, 1998, p. 139-144.

Research output: Contribution to journalArticleScientificpeer-review

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AB - In the present study theapaHpolymorphism in clinicalA. actinomycetemcomitansisolates were investigated in relation to their serotype and periodontal status of the donor subjects. The material included 122A. actinomycetemcomitansisolates representing serotypes a–e from 103 periodontally healthy and diseased subjects. TheapaHpolymorphism was investigated by both restriction analysis of the specific PCR amplification product and also by sequencing of PCR amplification products from selected clinical isolates. AnapaHspecific PCR amplification product was obtained from all isolates but the restriction patterns of the amplification products varied. Serotype c and genogroup 2 within serotype e formed genetically distinct groups, whereas isolates of serotype a, b, d and genogroup 1 within serotype e could not be separated from each other based on theapaHrestriction analysis. No relation between the restriction pattern ofapaHand the periodontal status of the individuals was detected. These results indicate that serotype c isolates form a uniformly distinct group withinA. actinomycetemcomitansand that a subpopulation of serotype e isolates clearly diverge from all otherA. actinomycetemcomitansisolates.

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