Application of aptamers in diagnostics, drug-delivery and imaging

Chetan Chandola, Sheetal Kalme, Marco G. Casteleijn, Arto Urtti, Muniasamy Neerathilingam (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target with high specificity and affinity. Although aptamers are analogous to antibodies for a wide range of target recognition and variety of applications, they have significant advantages over antibodies. Since aptamers have recently emerged as a class of biomolecules with an application in a wide array of fields, we need to summarize the latest developments herein. In this review we will discuss about the latest developments in using aptamers in diagnostics, drug delivery and imaging. We begin with diagnostics, discussing the application of aptamers for the detection of infective agents itself, antigens/toxins (bacteria), biomarkers (cancer), or a combination. The ease of conjugation and labelling of aptamers makes them a potential tool for diagnostics. Also, due to the reduced off-target effects of aptamers, their use as a potential drug delivery tool is emerging rapidly. Hence, we discuss their use in targeted delivery in conjugation with siRNAs, nanoparticles, liposomes, drugs and antibodies. Finally, we discuss about the conjugation strategies applicable for RNA and DNA aptamers for imaging. Their stability and self-assembly after heating makes them superior over protein-based binding molecules in terms of labelling and conjugation strategies.
Original languageEnglish
Pages (from-to)535-561
Number of pages27
JournalJournal of Biosciences
Volume41
Issue number3
DOIs
Publication statusPublished - 4 Aug 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Drug delivery
Nucleotide Aptamers
image analysis
Imaging techniques
drugs
Labeling
antibodies
Antibodies
Pharmaceutical Preparations
RNA
Single-Stranded DNA
Biomolecules
DNA
nanoparticles
Tumor Biomarkers
oligonucleotides
Protein Binding
Liposomes
Oligonucleotides
Nanoparticles

Keywords

  • Aptamer
  • cancer
  • conjugation-methods
  • diagnostics
  • drug-delivery
  • imaging

Cite this

Chandola, Chetan ; Kalme, Sheetal ; Casteleijn, Marco G. ; Urtti, Arto ; Neerathilingam, Muniasamy. / Application of aptamers in diagnostics, drug-delivery and imaging. In: Journal of Biosciences. 2016 ; Vol. 41, No. 3. pp. 535-561.
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Application of aptamers in diagnostics, drug-delivery and imaging. / Chandola, Chetan; Kalme, Sheetal; Casteleijn, Marco G.; Urtti, Arto; Neerathilingam, Muniasamy (Corresponding Author).

In: Journal of Biosciences, Vol. 41, No. 3, 04.08.2016, p. 535-561.

Research output: Contribution to journalArticleScientificpeer-review

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