Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 535-561 |
| Number of pages | 27 |
| Journal | Journal of Biosciences |
| Volume | 41 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 4 Aug 2016 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Aptamer
- cancer
- conjugation-methods
- diagnostics
- drug-delivery
- imaging
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Application of aptamers in diagnostics, drug-delivery and imaging. / Chandola, Chetan; Kalme, Sheetal; Casteleijn, Marco G.; Urtti, Arto; Neerathilingam, Muniasamy (Corresponding Author).
In: Journal of Biosciences, Vol. 41, No. 3, 04.08.2016, p. 535-561.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Application of aptamers in diagnostics, drug-delivery and imaging
AU - Chandola, Chetan
AU - Kalme, Sheetal
AU - Casteleijn, Marco G.
AU - Urtti, Arto
AU - Neerathilingam, Muniasamy
PY - 2016/8/4
Y1 - 2016/8/4
N2 - Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target with high specificity and affinity. Although aptamers are analogous to antibodies for a wide range of target recognition and variety of applications, they have significant advantages over antibodies. Since aptamers have recently emerged as a class of biomolecules with an application in a wide array of fields, we need to summarize the latest developments herein. In this review we will discuss about the latest developments in using aptamers in diagnostics, drug delivery and imaging. We begin with diagnostics, discussing the application of aptamers for the detection of infective agents itself, antigens/toxins (bacteria), biomarkers (cancer), or a combination. The ease of conjugation and labelling of aptamers makes them a potential tool for diagnostics. Also, due to the reduced off-target effects of aptamers, their use as a potential drug delivery tool is emerging rapidly. Hence, we discuss their use in targeted delivery in conjugation with siRNAs, nanoparticles, liposomes, drugs and antibodies. Finally, we discuss about the conjugation strategies applicable for RNA and DNA aptamers for imaging. Their stability and self-assembly after heating makes them superior over protein-based binding molecules in terms of labelling and conjugation strategies.
AB - Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target with high specificity and affinity. Although aptamers are analogous to antibodies for a wide range of target recognition and variety of applications, they have significant advantages over antibodies. Since aptamers have recently emerged as a class of biomolecules with an application in a wide array of fields, we need to summarize the latest developments herein. In this review we will discuss about the latest developments in using aptamers in diagnostics, drug delivery and imaging. We begin with diagnostics, discussing the application of aptamers for the detection of infective agents itself, antigens/toxins (bacteria), biomarkers (cancer), or a combination. The ease of conjugation and labelling of aptamers makes them a potential tool for diagnostics. Also, due to the reduced off-target effects of aptamers, their use as a potential drug delivery tool is emerging rapidly. Hence, we discuss their use in targeted delivery in conjugation with siRNAs, nanoparticles, liposomes, drugs and antibodies. Finally, we discuss about the conjugation strategies applicable for RNA and DNA aptamers for imaging. Their stability and self-assembly after heating makes them superior over protein-based binding molecules in terms of labelling and conjugation strategies.
KW - Aptamer
KW - cancer
KW - conjugation-methods
KW - diagnostics
KW - drug-delivery
KW - imaging
UR - http://www.scopus.com/inward/record.url?scp=84982840435&partnerID=8YFLogxK
U2 - 10.1007/s12038-016-9632-y
DO - 10.1007/s12038-016-9632-y
M3 - Article
VL - 41
SP - 535
EP - 561
JO - Journal of Biosciences
JF - Journal of Biosciences
SN - 0250-5991
IS - 3
ER -