Abstract
This publication is a summary of seven research reports dealing with the
application of High Performance Liquid Chromatography (HPLC) to the isolation
of microbiologically active compounds in heated neutral glucose and fructose
solutions and in aqueous extracts of the mushroom Lactarius necator.In both
cases the starting point was a micro biologically measurable activity which
was used as detection method during the isolation procedures.The formation of
antimicrobial activity in neutral aqueous glucose and fructose solutions was
the first object of study.Starting from the original solutions and by
chromatographing them twice the compounds responsible for the antimicrobial
activity could be recovered in crystalline form.The first separation step was
an ordinary reverse phase chromatography, whereas the second was specific for
these compounds and involved the application of a gel permeation
chromatography column for reverse phase separation.As a result the compounds
responsible for the antimicrobial activity were identified as various phenolic
compounds.The aqueous extract of the edible mushroom Lactarius necator has
been shown to exhibit strong mutagenic activity in the Ames Salmonella assay.
The compound respon sible for this activity was isolated by HPLC This
compound, designated here as necatorin was shown to be one of the strongest
mutagens hitherto found in nature (6000 revertants/ g with the tester strain
TA100).Necatorin was found to be rather stable, so that the normal methods
applied in the preparation of mushrooms for consumption blanching, brining,
pickling or freezing do not remove or inactivate it efficiently.Necatorin is a
normal constituent of the mushroom with a rather high initial concentration
(3...20 mg/kg fresh weight), and not an accidental contamination.In both the
systems studied the application of HPLC in reverse phase mode made possible
the use of the original microbial activity test as detector with minimal
manipulation of the isolated fractions.Therefore investigations could be
directed to the correct fractions in these complex mixtures.
Original language | English |
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Qualification | Doctor Degree |
Awarding Institution |
|
Award date | 3 Jun 1986 |
Place of Publication | Espoo |
Publisher | |
Print ISBNs | 951-38-2589-2 |
Publication status | Published - 1986 |
MoE publication type | G4 Doctoral dissertation (monograph) |
Keywords
- high performance
- liquid chromatography
- HPLC
- extracts
- Lactarius necator
- mutagens
- antiinfective agents