Application of paper-supported printed gold electrodes for impedimetric immunosensor development

P. Ihalainen (Corresponding Author), Himadri Majumdar (Corresponding Author), T. Viitala, B. Törngren, T. Näreoja, A. Määttänen, J. Sarfraz, H. Härmä, M. Yliperttula, R. Österbacka, J. Peltonen

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.
Original languageEnglish
Pages (from-to)1-17
Number of pages16
JournalBiosensors
Volume3
Issue number1
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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Immunosensors
Gold
Electrodes
Biosensing Techniques
Biosensors
Point-of-Care Systems
Photoelectron Spectroscopy
Printing
Ink
Streptavidin
Surface Plasmon Resonance
Atomic Force Microscopy
Surface plasmon resonance
Substrates
Biotin
Nanoparticles
Anti-Idiotypic Antibodies
Atomic force microscopy
X ray photoelectron spectroscopy
Antigens

Keywords

  • paper electronics
  • nanoparticles
  • inkjet printing
  • immunoassays
  • impedance spectroscopy

Cite this

Ihalainen, P., Majumdar, H., Viitala, T., Törngren, B., Näreoja, T., Määttänen, A., ... Peltonen, J. (2013). Application of paper-supported printed gold electrodes for impedimetric immunosensor development. Biosensors, 3(1), 1-17. https://doi.org/10.3390/bios3010001
Ihalainen, P. ; Majumdar, Himadri ; Viitala, T. ; Törngren, B. ; Näreoja, T. ; Määttänen, A. ; Sarfraz, J. ; Härmä, H. ; Yliperttula, M. ; Österbacka, R. ; Peltonen, J. / Application of paper-supported printed gold electrodes for impedimetric immunosensor development. In: Biosensors. 2013 ; Vol. 3, No. 1. pp. 1-17.
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abstract = "In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.",
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Ihalainen, P, Majumdar, H, Viitala, T, Törngren, B, Näreoja, T, Määttänen, A, Sarfraz, J, Härmä, H, Yliperttula, M, Österbacka, R & Peltonen, J 2013, 'Application of paper-supported printed gold electrodes for impedimetric immunosensor development', Biosensors, vol. 3, no. 1, pp. 1-17. https://doi.org/10.3390/bios3010001

Application of paper-supported printed gold electrodes for impedimetric immunosensor development. / Ihalainen, P. (Corresponding Author); Majumdar, Himadri (Corresponding Author); Viitala, T.; Törngren, B.; Näreoja, T.; Määttänen, A.; Sarfraz, J.; Härmä, H.; Yliperttula, M.; Österbacka, R.; Peltonen, J.

In: Biosensors, Vol. 3, No. 1, 2013, p. 1-17.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Application of paper-supported printed gold electrodes for impedimetric immunosensor development

AU - Ihalainen, P.

AU - Majumdar, Himadri

AU - Viitala, T.

AU - Törngren, B.

AU - Näreoja, T.

AU - Määttänen, A.

AU - Sarfraz, J.

AU - Härmä, H.

AU - Yliperttula, M.

AU - Österbacka, R.

AU - Peltonen, J.

PY - 2013

Y1 - 2013

N2 - In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

AB - In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

KW - paper electronics

KW - nanoparticles

KW - inkjet printing

KW - immunoassays

KW - impedance spectroscopy

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DO - 10.3390/bios3010001

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JO - Biosensors

JF - Biosensors

SN - 2079-6374

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