Application of paper-supported printed gold electrodes for impedimetric immunosensor development

P. Ihalainen (Corresponding Author), Himadri Majumdar (Corresponding Author), T. Viitala, B. Törngren, T. Näreoja, A. Määttänen, J. Sarfraz, H. Härmä, M. Yliperttula, R. Österbacka, J. Peltonen

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Abstract

In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.
Original languageEnglish
Pages (from-to)1-17
Number of pages16
JournalBiosensors
Volume3
Issue number1
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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Keywords

  • paper electronics
  • nanoparticles
  • inkjet printing
  • immunoassays
  • impedance spectroscopy

Cite this

Ihalainen, P., Majumdar, H., Viitala, T., Törngren, B., Näreoja, T., Määttänen, A., Sarfraz, J., Härmä, H., Yliperttula, M., Österbacka, R., & Peltonen, J. (2013). Application of paper-supported printed gold electrodes for impedimetric immunosensor development. Biosensors, 3(1), 1-17. https://doi.org/10.3390/bios3010001