Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems

Annikka Mustranta, Pirkko Forssell, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

88 Citations (Scopus)

Abstract

Lipases from Candida cylindracea, Aspergillus niger, and Pseudomonas fluorescens were immobilized by adsorption on anion-exchange resin and diatomaceous earth using buffer or hexane as a reaction medium. The enzyme preparations were tested in the transesterification of triolein with lauric acid and the esterification of lauric acid with different alcohols. Immobilized C. cylindracea preparations were more active when hexane was used as the reaction medium, and anion-exchange resin was a better support than diatomaceous earth. Hexane was also a better immobilization medium for A. niger lipase. No difference was observed in the lipase activity of P. fluorescens lipase immobilized in different ways. The synthetic activities of the immobilized enzymes could be predicted from their hydrolytic activities: the higher the hydrolytic activity, the higher the synthetic activity. There was no direct correlation, however, between the lipolytic and the transesterification activities.

Original languageEnglish
Pages (from-to)133 - 139
Number of pages7
JournalEnzyme and Microbial Technology
Volume15
Issue number2
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

Fingerprint

lauric acid
Esterification
Transesterification
Lipases
Lipase
Hexanes
Hexane
Anion Exchange Resins
Diatomaceous Earth
Pseudomonas fluorescens
Aspergillus niger
Ion exchange
Negative ions
Enzymes
Resins
Earth (planet)
Triolein
Immobilized Enzymes
Acids
Candida

Cite this

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title = "Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems",
abstract = "Lipases from Candida cylindracea, Aspergillus niger, and Pseudomonas fluorescens were immobilized by adsorption on anion-exchange resin and diatomaceous earth using buffer or hexane as a reaction medium. The enzyme preparations were tested in the transesterification of triolein with lauric acid and the esterification of lauric acid with different alcohols. Immobilized C. cylindracea preparations were more active when hexane was used as the reaction medium, and anion-exchange resin was a better support than diatomaceous earth. Hexane was also a better immobilization medium for A. niger lipase. No difference was observed in the lipase activity of P. fluorescens lipase immobilized in different ways. The synthetic activities of the immobilized enzymes could be predicted from their hydrolytic activities: the higher the hydrolytic activity, the higher the synthetic activity. There was no direct correlation, however, between the lipolytic and the transesterification activities.",
author = "Annikka Mustranta and Pirkko Forssell and Kaisa Poutanen",
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Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems. / Mustranta, Annikka; Forssell, Pirkko; Poutanen, Kaisa.

In: Enzyme and Microbial Technology, Vol. 15, No. 2, 1993, p. 133 - 139.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems

AU - Mustranta, Annikka

AU - Forssell, Pirkko

AU - Poutanen, Kaisa

N1 - Project code: BIO2013

PY - 1993

Y1 - 1993

N2 - Lipases from Candida cylindracea, Aspergillus niger, and Pseudomonas fluorescens were immobilized by adsorption on anion-exchange resin and diatomaceous earth using buffer or hexane as a reaction medium. The enzyme preparations were tested in the transesterification of triolein with lauric acid and the esterification of lauric acid with different alcohols. Immobilized C. cylindracea preparations were more active when hexane was used as the reaction medium, and anion-exchange resin was a better support than diatomaceous earth. Hexane was also a better immobilization medium for A. niger lipase. No difference was observed in the lipase activity of P. fluorescens lipase immobilized in different ways. The synthetic activities of the immobilized enzymes could be predicted from their hydrolytic activities: the higher the hydrolytic activity, the higher the synthetic activity. There was no direct correlation, however, between the lipolytic and the transesterification activities.

AB - Lipases from Candida cylindracea, Aspergillus niger, and Pseudomonas fluorescens were immobilized by adsorption on anion-exchange resin and diatomaceous earth using buffer or hexane as a reaction medium. The enzyme preparations were tested in the transesterification of triolein with lauric acid and the esterification of lauric acid with different alcohols. Immobilized C. cylindracea preparations were more active when hexane was used as the reaction medium, and anion-exchange resin was a better support than diatomaceous earth. Hexane was also a better immobilization medium for A. niger lipase. No difference was observed in the lipase activity of P. fluorescens lipase immobilized in different ways. The synthetic activities of the immobilized enzymes could be predicted from their hydrolytic activities: the higher the hydrolytic activity, the higher the synthetic activity. There was no direct correlation, however, between the lipolytic and the transesterification activities.

U2 - 10.1016/0141-0229(93)90037-3

DO - 10.1016/0141-0229(93)90037-3

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JO - Enzyme and Microbial Technology

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