Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems

Annikka Mustranta, Pirkko Forssell, Kaisa Poutanen

    Research output: Contribution to journalArticleScientificpeer-review

    90 Citations (Scopus)

    Abstract

    Lipases from Candida cylindracea, Aspergillus niger, and Pseudomonas fluorescens were immobilized by adsorption on anion-exchange resin and diatomaceous earth using buffer or hexane as a reaction medium. The enzyme preparations were tested in the transesterification of triolein with lauric acid and the esterification of lauric acid with different alcohols. Immobilized C. cylindracea preparations were more active when hexane was used as the reaction medium, and anion-exchange resin was a better support than diatomaceous earth. Hexane was also a better immobilization medium for A. niger lipase. No difference was observed in the lipase activity of P. fluorescens lipase immobilized in different ways. The synthetic activities of the immobilized enzymes could be predicted from their hydrolytic activities: the higher the hydrolytic activity, the higher the synthetic activity. There was no direct correlation, however, between the lipolytic and the transesterification activities.

    Original languageEnglish
    Pages (from-to)133 - 139
    Number of pages7
    JournalEnzyme and Microbial Technology
    Volume15
    Issue number2
    DOIs
    Publication statusPublished - 1993
    MoE publication typeA1 Journal article-refereed

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