Abstract
Fast and sensitive detection of cocaine in saliva is realized utilizing a
surface-sensitive fluorescence measurement platform. The platform is
based on a polystyrene parabolic lens that enables the simultaneous
application of total internal reflection excitation (TIR) and
supercritical angle fluorescence detection (SAF), which results in
extreme surface sensitivity in the measurements. The molecular
recognition takes place in a scheme, where cocaine molecules to be
detected generate a displacement of the labelled anti-cocaine antibodies
from the cocaine-BSA-conjugate molecules immobilized on a surface. The
results with untreated saliva spiked with cocaine demonstrate that by
monitoring the dissociation process in real time, cocaine concentrations
down to 1 ng/mL can be detected within 60 s.
Original language | English |
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Pages (from-to) | 968-971 |
Number of pages | 4 |
Journal | Procedia Engineering |
Volume | 25 |
DOIs | |
Publication status | Published - 2011 |
MoE publication type | A1 Journal article-refereed |
Event | 25th Eurosensors Conference - Athens, Greece Duration: 4 Sept 2011 → 7 Sept 2011 |
Keywords
- Antibody displacement
- cocaine
- drugs
- on-site detection
- oral fluids
- SAF
- saliva
- surface sensitive fluorescence
- TIR