Applying a surface-sensitive fluorescence method to fast on-site detection of cocaine in saliva

Hannu Välimäki (Corresponding Author), Sanna Auer, Ann-Charlotte Hellgren, Kirsi Tappura

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    Fast and sensitive detection of cocaine in saliva is realized utilizing a surface-sensitive fluorescence measurement platform. The platform is based on a polystyrene parabolic lens that enables the simultaneous application of total internal reflection excitation (TIR) and supercritical angle fluorescence detection (SAF), which results in extreme surface sensitivity in the measurements. The molecular recognition takes place in a scheme, where cocaine molecules to be detected generate a displacement of the labelled anti-cocaine antibodies from the cocaine-BSA-conjugate molecules immobilized on a surface. The results with untreated saliva spiked with cocaine demonstrate that by monitoring the dissociation process in real time, cocaine concentrations down to 1 ng/mL can be detected within 60 s.
    Original languageEnglish
    Pages (from-to)968-971
    Number of pages4
    JournalProcedia Engineering
    Volume25
    DOIs
    Publication statusPublished - 2011
    MoE publication typeA1 Journal article-refereed
    Event25th Eurosensors Conference - Athens, Greece
    Duration: 4 Sep 20117 Sep 2011

    Keywords

    • Antibody displacement
    • cocaine
    • drugs
    • on-site detection
    • oral fluids
    • SAF
    • saliva
    • surface sensitive fluorescence
    • TIR

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