Applying a surface-sensitive fluorescence method to fast on-site detection of cocaine in saliva

Hannu Välimäki (Corresponding Author), Sanna Auer, Ann-Charlotte Hellgren, Kirsi Tappura

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Fast and sensitive detection of cocaine in saliva is realized utilizing a surface-sensitive fluorescence measurement platform. The platform is based on a polystyrene parabolic lens that enables the simultaneous application of total internal reflection excitation (TIR) and supercritical angle fluorescence detection (SAF), which results in extreme surface sensitivity in the measurements. The molecular recognition takes place in a scheme, where cocaine molecules to be detected generate a displacement of the labelled anti-cocaine antibodies from the cocaine-BSA-conjugate molecules immobilized on a surface. The results with untreated saliva spiked with cocaine demonstrate that by monitoring the dissociation process in real time, cocaine concentrations down to 1 ng/mL can be detected within 60 s.
Original languageEnglish
Pages (from-to)968-971
Number of pages4
JournalProcedia Engineering
Volume25
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed
Event25th Eurosensors Conference - Athens, Greece
Duration: 4 Sep 20117 Sep 2011

Fingerprint

Fluorescence
Molecular recognition
Molecules
Antibodies
Lenses
Polystyrenes
Monitoring

Keywords

  • Antibody displacement
  • cocaine
  • drugs
  • on-site detection
  • oral fluids
  • SAF
  • saliva
  • surface sensitive fluorescence
  • TIR

Cite this

Välimäki, Hannu ; Auer, Sanna ; Hellgren, Ann-Charlotte ; Tappura, Kirsi. / Applying a surface-sensitive fluorescence method to fast on-site detection of cocaine in saliva. In: Procedia Engineering. 2011 ; Vol. 25. pp. 968-971.
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Applying a surface-sensitive fluorescence method to fast on-site detection of cocaine in saliva. / Välimäki, Hannu (Corresponding Author); Auer, Sanna; Hellgren, Ann-Charlotte; Tappura, Kirsi.

In: Procedia Engineering, Vol. 25, 2011, p. 968-971.

Research output: Contribution to journalArticleScientificpeer-review

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KW - Antibody displacement

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