Applying total internal reflection excitation and super critical angle fluorescence detection to a morphine assay

Hannu Välimäki (Corresponding Author), Timo Pulli, Kirsi Tappura

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

A surface-sensitive fluorescence measurement platform is utilised in the detection of morphine. The platform is based on a polystyrene parabolic lens that enables the simultaneous application of total internal reflection excitation and supercritical angle fluorescence detection in the measurements. The molecular recognition of morphine is based on two antibodies, one against morphine and the other against the immune complex formed between the anti-morphine antibody and a morphine molecule. The antibodies are applied in a sandwich-like format in a one-step test, where the molecular binding onto a liquid-solid-interface is monitored in real time. Morphine concentrations between 0.6 and 18.2 ng/mL were reliably determined in 60 s, while concentrations down to 2.7 ng/mL were detected already in 20 s. With appropriate recognition molecules the technique is applicable also to other drugs and small analytes.
Original languageEnglish
Pages (from-to)1003-1008
Number of pages6
JournalJournal of Fluorescence
Volume20
Issue number5
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Morphine
Assays
Fluorescence
drug
Antibodies
Molecular recognition
Molecules
Polystyrenes
Antigen-Antibody Complex
Exercise Test
Lenses
Anti-Idiotypic Antibodies
Liquids
Pharmaceutical Preparations
time

Keywords

  • Drug testing
  • Fluoroimmunoassays
  • Morphine
  • Supercritical angle fluorescence (SAF)
  • Total internal reflection fluorescence (TIRF)

Cite this

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title = "Applying total internal reflection excitation and super critical angle fluorescence detection to a morphine assay",
abstract = "A surface-sensitive fluorescence measurement platform is utilised in the detection of morphine. The platform is based on a polystyrene parabolic lens that enables the simultaneous application of total internal reflection excitation and supercritical angle fluorescence detection in the measurements. The molecular recognition of morphine is based on two antibodies, one against morphine and the other against the immune complex formed between the anti-morphine antibody and a morphine molecule. The antibodies are applied in a sandwich-like format in a one-step test, where the molecular binding onto a liquid-solid-interface is monitored in real time. Morphine concentrations between 0.6 and 18.2 ng/mL were reliably determined in 60 s, while concentrations down to 2.7 ng/mL were detected already in 20 s. With appropriate recognition molecules the technique is applicable also to other drugs and small analytes.",
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Applying total internal reflection excitation and super critical angle fluorescence detection to a morphine assay. / Välimäki, Hannu (Corresponding Author); Pulli, Timo; Tappura, Kirsi.

In: Journal of Fluorescence, Vol. 20, No. 5, 2010, p. 1003-1008.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Välimäki, Hannu

AU - Pulli, Timo

AU - Tappura, Kirsi

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KW - Fluoroimmunoassays

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