Aqueous and non-aqueous capillary electrophoresis of polar drugs

Heli Sirén (Corresponding Author), Tarja Hiissa, Yuan Min

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Aqueous and non-aqueous capillary electrophoresis (CE) systems were employed for the separation of basic and acidic polar drugs using capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MECC) techniques. This work was performed to determine the differences in selectivity, after chemical adjustment of the media, of the separation of closely related drugs (by measurement of separation efficiency, resolution and detection) in aqueous and non-aqueous solvents. Various electrolytes were tested in aqueous and organic solvent media. The best separation in aqueous CZE was obtained in 25 mM phosphate solution at pH 7.3. In aqueous MECC, the best performance was obtained in pH 10.5 buffer containing glycine and dodecyl sulfate. Non-aqueous CE separations were performed in electrolyte mixtures containing methanol–acetonitrile, ethanol–acetonitrile and propan-2-ol–acetonitrile (50∶49, v/v) modified by ammonium acetate and water-free glacial acetic acid. Acetone was also used instead of acetonitrile in the separation media. In non-aqueous CE systems, the effect of water in the non-aqueous sample and as part of the electrolyte solution played a significant role in the separation of polar anionic drugs.
Original languageEnglish
Pages (from-to)1561 - 1568
Number of pages8
JournalThe Analyst
Volume125
Issue number9
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

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Capillary electrophoresis
Capillary Electrophoresis
electrokinesis
drug
Electrolytes
Pharmaceutical Preparations
electrolyte
Chromatography
Electrophoresis
chromatography
Water
Acetone
Acetic Acid
Glycine
Buffers
Phosphates
Acetonitrile
acetone
Acetic acid
acetic acid

Cite this

Sirén, Heli ; Hiissa, Tarja ; Min, Yuan. / Aqueous and non-aqueous capillary electrophoresis of polar drugs. In: The Analyst. 2000 ; Vol. 125, No. 9. pp. 1561 - 1568.
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title = "Aqueous and non-aqueous capillary electrophoresis of polar drugs",
abstract = "Aqueous and non-aqueous capillary electrophoresis (CE) systems were employed for the separation of basic and acidic polar drugs using capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MECC) techniques. This work was performed to determine the differences in selectivity, after chemical adjustment of the media, of the separation of closely related drugs (by measurement of separation efficiency, resolution and detection) in aqueous and non-aqueous solvents. Various electrolytes were tested in aqueous and organic solvent media. The best separation in aqueous CZE was obtained in 25 mM phosphate solution at pH 7.3. In aqueous MECC, the best performance was obtained in pH 10.5 buffer containing glycine and dodecyl sulfate. Non-aqueous CE separations were performed in electrolyte mixtures containing methanol–acetonitrile, ethanol–acetonitrile and propan-2-ol–acetonitrile (50∶49, v/v) modified by ammonium acetate and water-free glacial acetic acid. Acetone was also used instead of acetonitrile in the separation media. In non-aqueous CE systems, the effect of water in the non-aqueous sample and as part of the electrolyte solution played a significant role in the separation of polar anionic drugs.",
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Sirén, H, Hiissa, T & Min, Y 2000, 'Aqueous and non-aqueous capillary electrophoresis of polar drugs', The Analyst, vol. 125, no. 9, pp. 1561 - 1568. https://doi.org/10.1039/A910305H

Aqueous and non-aqueous capillary electrophoresis of polar drugs. / Sirén, Heli (Corresponding Author); Hiissa, Tarja; Min, Yuan.

In: The Analyst, Vol. 125, No. 9, 2000, p. 1561 - 1568.

Research output: Contribution to journalArticleScientificpeer-review

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AB - Aqueous and non-aqueous capillary electrophoresis (CE) systems were employed for the separation of basic and acidic polar drugs using capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MECC) techniques. This work was performed to determine the differences in selectivity, after chemical adjustment of the media, of the separation of closely related drugs (by measurement of separation efficiency, resolution and detection) in aqueous and non-aqueous solvents. Various electrolytes were tested in aqueous and organic solvent media. The best separation in aqueous CZE was obtained in 25 mM phosphate solution at pH 7.3. In aqueous MECC, the best performance was obtained in pH 10.5 buffer containing glycine and dodecyl sulfate. Non-aqueous CE separations were performed in electrolyte mixtures containing methanol–acetonitrile, ethanol–acetonitrile and propan-2-ol–acetonitrile (50∶49, v/v) modified by ammonium acetate and water-free glacial acetic acid. Acetone was also used instead of acetonitrile in the separation media. In non-aqueous CE systems, the effect of water in the non-aqueous sample and as part of the electrolyte solution played a significant role in the separation of polar anionic drugs.

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