Arctic bramble cell cultures as a source of berry phenolics

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Berries are rich in phenolic compounds with beneficial biological properties for human health. Arctic bramble (Rubus arcticus) grows in borealic zone, and its ruby red berries are well known for good taste and flavour. In addition, arctic bramble is rich in bioactive phenolic compounds, especially the complex phenolic polymer ellagitannin, which is reported to have e.g. in vitro anti-oxidant activities and antimicrobial effects against human pathogens. The colour of arctic bramble originates from anthocyanins, which also possess variable beneficial effects on human health. The crop of both wild and cultivated arctic bramble is very low, and therefore cell cultures are the potential choice for production of already characterized and novel secondary metabolites for food and pharmaceutical purposes. Arctic bramble cell cultures were established from cuts of sterile in vitro leaves on medium with plant hormones kinetin and NAA (alpha-Naphtalen-acetic acid). Callus lines were grown on the hormone medium, and good growth and bright colours were used for selection criteria for maintenance and initiation of suspension cultures. Phenolic compounds were measured from stable callus and suspension cultures obtained, and selected culture was used for elicitation experiment aiming to increase production of phenolic secondary metabolites. Elicitors used were methyl jasmonate, ethephon, S-ABA and chitosan, and they were introduced in the cultures in late logarithmic growth phase. Samples collected at different time points were filtered, and the cell mass was freeze-dried and extracted with methanol. Freeze-dried extracts were analysed by HPLC-DAD. In callus and suspension cultures phenolic acids were the main phenolics detected in methanol extracts. Elicitation clearly enhanced the production of some phenolic compounds in suspension cultures, and elicitation with S-ABA also promoted synthesis of a new phenolic compound, which still needs to be characterized.
Original languageEnglish
Title of host publicationPlants for Human Health in the Post-Genome Era
Subtitle of host publicationPSE Congress
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages87-87
ISBN (Electronic)978-951-38-6322-7
ISBN (Print)978-951-38-6321-0
Publication statusPublished - 2007
EventPSE Congress: Plants for Human Health in the Post-Genome Era - Helsinki, Finland
Duration: 26 Aug 200729 Aug 2007

Publication series

NameVTT Symposium
PublisherVTT
Number249
ISSN (Print)0357-9387
ISSN (Electronic)1445-0873

Conference

ConferencePSE Congress: Plants for Human Health in the Post-Genome Era
CountryFinland
CityHelsinki
Period26/08/0729/08/07

Fingerprint

small fruits
Arctic region
phenolic compounds
cell culture
callus
secondary metabolites
human health
abscisic acid
Rubus arcticus
methanol
color
ethephon
extracts
methyl jasmonate
anti-infective properties
selection criteria
chitosan
kinetin
phenolic acids
plant hormones

Cite this

Nohynek, L., Nissilä, R., Seppänen-Laakso, T., Oksman-Caldentey, K-M., & Puupponen-Pimiä, R. (2007). Arctic bramble cell cultures as a source of berry phenolics. In Plants for Human Health in the Post-Genome Era: PSE Congress (pp. 87-87). [C1] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 249
Nohynek, Liisa ; Nissilä, R. ; Seppänen-Laakso, Tuulikki ; Oksman-Caldentey, Kirsi-Marja ; Puupponen-Pimiä, Riitta. / Arctic bramble cell cultures as a source of berry phenolics. Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 87-87 (VTT Symposium; No. 249).
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abstract = "Berries are rich in phenolic compounds with beneficial biological properties for human health. Arctic bramble (Rubus arcticus) grows in borealic zone, and its ruby red berries are well known for good taste and flavour. In addition, arctic bramble is rich in bioactive phenolic compounds, especially the complex phenolic polymer ellagitannin, which is reported to have e.g. in vitro anti-oxidant activities and antimicrobial effects against human pathogens. The colour of arctic bramble originates from anthocyanins, which also possess variable beneficial effects on human health. The crop of both wild and cultivated arctic bramble is very low, and therefore cell cultures are the potential choice for production of already characterized and novel secondary metabolites for food and pharmaceutical purposes. Arctic bramble cell cultures were established from cuts of sterile in vitro leaves on medium with plant hormones kinetin and NAA (alpha-Naphtalen-acetic acid). Callus lines were grown on the hormone medium, and good growth and bright colours were used for selection criteria for maintenance and initiation of suspension cultures. Phenolic compounds were measured from stable callus and suspension cultures obtained, and selected culture was used for elicitation experiment aiming to increase production of phenolic secondary metabolites. Elicitors used were methyl jasmonate, ethephon, S-ABA and chitosan, and they were introduced in the cultures in late logarithmic growth phase. Samples collected at different time points were filtered, and the cell mass was freeze-dried and extracted with methanol. Freeze-dried extracts were analysed by HPLC-DAD. In callus and suspension cultures phenolic acids were the main phenolics detected in methanol extracts. Elicitation clearly enhanced the production of some phenolic compounds in suspension cultures, and elicitation with S-ABA also promoted synthesis of a new phenolic compound, which still needs to be characterized.",
author = "Liisa Nohynek and R. Nissil{\"a} and Tuulikki Sepp{\"a}nen-Laakso and Kirsi-Marja Oksman-Caldentey and Riitta Puupponen-Pimi{\"a}",
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Nohynek, L, Nissilä, R, Seppänen-Laakso, T, Oksman-Caldentey, K-M & Puupponen-Pimiä, R 2007, Arctic bramble cell cultures as a source of berry phenolics. in Plants for Human Health in the Post-Genome Era: PSE Congress., C1, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 249, pp. 87-87, PSE Congress: Plants for Human Health in the Post-Genome Era, Helsinki, Finland, 26/08/07.

Arctic bramble cell cultures as a source of berry phenolics. / Nohynek, Liisa; Nissilä, R.; Seppänen-Laakso, Tuulikki; Oksman-Caldentey, Kirsi-Marja; Puupponen-Pimiä, Riitta.

Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo : VTT Technical Research Centre of Finland, 2007. p. 87-87 C1 (VTT Symposium; No. 249).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Arctic bramble cell cultures as a source of berry phenolics

AU - Nohynek, Liisa

AU - Nissilä, R.

AU - Seppänen-Laakso, Tuulikki

AU - Oksman-Caldentey, Kirsi-Marja

AU - Puupponen-Pimiä, Riitta

PY - 2007

Y1 - 2007

N2 - Berries are rich in phenolic compounds with beneficial biological properties for human health. Arctic bramble (Rubus arcticus) grows in borealic zone, and its ruby red berries are well known for good taste and flavour. In addition, arctic bramble is rich in bioactive phenolic compounds, especially the complex phenolic polymer ellagitannin, which is reported to have e.g. in vitro anti-oxidant activities and antimicrobial effects against human pathogens. The colour of arctic bramble originates from anthocyanins, which also possess variable beneficial effects on human health. The crop of both wild and cultivated arctic bramble is very low, and therefore cell cultures are the potential choice for production of already characterized and novel secondary metabolites for food and pharmaceutical purposes. Arctic bramble cell cultures were established from cuts of sterile in vitro leaves on medium with plant hormones kinetin and NAA (alpha-Naphtalen-acetic acid). Callus lines were grown on the hormone medium, and good growth and bright colours were used for selection criteria for maintenance and initiation of suspension cultures. Phenolic compounds were measured from stable callus and suspension cultures obtained, and selected culture was used for elicitation experiment aiming to increase production of phenolic secondary metabolites. Elicitors used were methyl jasmonate, ethephon, S-ABA and chitosan, and they were introduced in the cultures in late logarithmic growth phase. Samples collected at different time points were filtered, and the cell mass was freeze-dried and extracted with methanol. Freeze-dried extracts were analysed by HPLC-DAD. In callus and suspension cultures phenolic acids were the main phenolics detected in methanol extracts. Elicitation clearly enhanced the production of some phenolic compounds in suspension cultures, and elicitation with S-ABA also promoted synthesis of a new phenolic compound, which still needs to be characterized.

AB - Berries are rich in phenolic compounds with beneficial biological properties for human health. Arctic bramble (Rubus arcticus) grows in borealic zone, and its ruby red berries are well known for good taste and flavour. In addition, arctic bramble is rich in bioactive phenolic compounds, especially the complex phenolic polymer ellagitannin, which is reported to have e.g. in vitro anti-oxidant activities and antimicrobial effects against human pathogens. The colour of arctic bramble originates from anthocyanins, which also possess variable beneficial effects on human health. The crop of both wild and cultivated arctic bramble is very low, and therefore cell cultures are the potential choice for production of already characterized and novel secondary metabolites for food and pharmaceutical purposes. Arctic bramble cell cultures were established from cuts of sterile in vitro leaves on medium with plant hormones kinetin and NAA (alpha-Naphtalen-acetic acid). Callus lines were grown on the hormone medium, and good growth and bright colours were used for selection criteria for maintenance and initiation of suspension cultures. Phenolic compounds were measured from stable callus and suspension cultures obtained, and selected culture was used for elicitation experiment aiming to increase production of phenolic secondary metabolites. Elicitors used were methyl jasmonate, ethephon, S-ABA and chitosan, and they were introduced in the cultures in late logarithmic growth phase. Samples collected at different time points were filtered, and the cell mass was freeze-dried and extracted with methanol. Freeze-dried extracts were analysed by HPLC-DAD. In callus and suspension cultures phenolic acids were the main phenolics detected in methanol extracts. Elicitation clearly enhanced the production of some phenolic compounds in suspension cultures, and elicitation with S-ABA also promoted synthesis of a new phenolic compound, which still needs to be characterized.

M3 - Conference abstract in proceedings

SN - 978-951-38-6321-0

T3 - VTT Symposium

SP - 87

EP - 87

BT - Plants for Human Health in the Post-Genome Era

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Nohynek L, Nissilä R, Seppänen-Laakso T, Oksman-Caldentey K-M, Puupponen-Pimiä R. Arctic bramble cell cultures as a source of berry phenolics. In Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo: VTT Technical Research Centre of Finland. 2007. p. 87-87. C1. (VTT Symposium; No. 249).