Abstract
Under a limited set of hitherto incompletely defined conditions, inhibition of respiration has been shown to cause transient oscillations in NAD(P)H fluorescence of yeast cells. In this paper, we apply a new method [1992, Anal. Biochem. 204, 118-132] for extraction of intracellular metabolites. This method involves spraying the cells into -40°C methanol; the neutral pH allows extraction of nearly all intracellular metabolites, including NADH. Close to the shift from glucose to ethanol as a growth substrate, the cells acquire a make-up amenable to sustained oscillations in intracellular concentrations of NADH and glycolytic intermediates such as glucose-6-phosphate. NADH was found to oscillate between 200 μM and 400 μM intracellular concentration. The cellular make-up determining the tendency to oscillate is 'remembered' by the cells after three hours of starvation.
| Original language | English |
|---|---|
| Pages (from-to) | 80-82 |
| Number of pages | 3 |
| Journal | FEBS Letters |
| Volume | 318 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 22 Feb 1993 |
| MoE publication type | Not Eligible |
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Keywords
- Glycolysis
- Intracellular metabolite
- Oscillation
- Yeast
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Around the growth phase transition S. cerevisiae's make-up favours sustained oscillations of intracellular metabolites. / Richard, Peter; Teusink, Bas; Westerhoff, Hans V.; van Dam, Karel (Corresponding Author).
In: FEBS Letters, Vol. 318, No. 1, 22.02.1993, p. 80-82.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Around the growth phase transition S. cerevisiae's make-up favours sustained oscillations of intracellular metabolites
AU - Richard, Peter
AU - Teusink, Bas
AU - Westerhoff, Hans V.
AU - van Dam, Karel
PY - 1993/2/22
Y1 - 1993/2/22
N2 - Under a limited set of hitherto incompletely defined conditions, inhibition of respiration has been shown to cause transient oscillations in NAD(P)H fluorescence of yeast cells. In this paper, we apply a new method [1992, Anal. Biochem. 204, 118-132] for extraction of intracellular metabolites. This method involves spraying the cells into -40°C methanol; the neutral pH allows extraction of nearly all intracellular metabolites, including NADH. Close to the shift from glucose to ethanol as a growth substrate, the cells acquire a make-up amenable to sustained oscillations in intracellular concentrations of NADH and glycolytic intermediates such as glucose-6-phosphate. NADH was found to oscillate between 200 μM and 400 μM intracellular concentration. The cellular make-up determining the tendency to oscillate is 'remembered' by the cells after three hours of starvation.
AB - Under a limited set of hitherto incompletely defined conditions, inhibition of respiration has been shown to cause transient oscillations in NAD(P)H fluorescence of yeast cells. In this paper, we apply a new method [1992, Anal. Biochem. 204, 118-132] for extraction of intracellular metabolites. This method involves spraying the cells into -40°C methanol; the neutral pH allows extraction of nearly all intracellular metabolites, including NADH. Close to the shift from glucose to ethanol as a growth substrate, the cells acquire a make-up amenable to sustained oscillations in intracellular concentrations of NADH and glycolytic intermediates such as glucose-6-phosphate. NADH was found to oscillate between 200 μM and 400 μM intracellular concentration. The cellular make-up determining the tendency to oscillate is 'remembered' by the cells after three hours of starvation.
KW - Glycolysis
KW - Intracellular metabolite
KW - Oscillation
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=0027446595&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(93)81332-T
DO - 10.1016/0014-5793(93)81332-T
M3 - Article
C2 - 8436231
AN - SCOPUS:0027446595
VL - 318
SP - 80
EP - 82
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -