Around the growth phase transition S. cerevisiae's make-up favours sustained oscillations of intracellular metabolites

Peter Richard, Bas Teusink, Hans V. Westerhoff, Karel van Dam (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

72 Citations (Scopus)

Abstract

Under a limited set of hitherto incompletely defined conditions, inhibition of respiration has been shown to cause transient oscillations in NAD(P)H fluorescence of yeast cells. In this paper, we apply a new method [1992, Anal. Biochem. 204, 118-132] for extraction of intracellular metabolites. This method involves spraying the cells into -40°C methanol; the neutral pH allows extraction of nearly all intracellular metabolites, including NADH. Close to the shift from glucose to ethanol as a growth substrate, the cells acquire a make-up amenable to sustained oscillations in intracellular concentrations of NADH and glycolytic intermediates such as glucose-6-phosphate. NADH was found to oscillate between 200 μM and 400 μM intracellular concentration. The cellular make-up determining the tendency to oscillate is 'remembered' by the cells after three hours of starvation.

Original languageEnglish
Pages (from-to)80-82
Number of pages3
JournalFEBS Letters
Volume318
Issue number1
DOIs
Publication statusPublished - 22 Feb 1993
MoE publication typeNot Eligible

Keywords

  • Glycolysis
  • Intracellular metabolite
  • Oscillation
  • Yeast

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