Array-in-well epitope mapping of phage-displayed antibodies

Urpo Lamminmäki; Gaurav Batra; Petri Saviranta

doi:10.1007/978-1-4939-7841-0_9

Array-in-well epitope mapping of phage-displayed antibodies

Urpo Lamminmäki^*, Gaurav Batra, Petri Saviranta

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

Abstract

Novel affinity reagents, such as single chain (scFv) antibody fragments, can be generated by isolating them from recombinant protein libraries using phage display selection. A successful selection process against a target protein can produce a number of binder candidates among which the desired binders are identified by screening and characterization of individual clones. Obtaining information on the binding properties, such as the binding epitope, already during the screening step helps to choose the most useful candidates for further development at early phase saving time and resources. To this end, we describe here an Array-in-Well-based screening procedure to perform activity testing and epitope mapping for filamentous phage-displayed scFvs in an integrated manner with a single assay.

Original language	English
Title of host publication	Epitope Mapping Protocols
Publisher	Springer
Pages	129-140
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-4939-7841-0_9
Publication status	Published - 1 Jan 2018
MoE publication type	A3 Part of a book or another research book

Publication series

Series	Methods in Molecular Biology
Volume	1785
ISSN	1064-3745

Keywords

Antibody discovery
Antibody phage library
Array-in-well (AinW)
Epitope mapping
Peptide array
Protein array

Access to Document

10.1007/978-1-4939-7841-0_9

Cite this

@inbook{f3ff9563b72e491d84bc9a8851846d98,

title = "Array-in-well epitope mapping of phage-displayed antibodies",

abstract = "Novel affinity reagents, such as single chain (scFv) antibody fragments, can be generated by isolating them from recombinant protein libraries using phage display selection. A successful selection process against a target protein can produce a number of binder candidates among which the desired binders are identified by screening and characterization of individual clones. Obtaining information on the binding properties, such as the binding epitope, already during the screening step helps to choose the most useful candidates for further development at early phase saving time and resources. To this end, we describe here an Array-in-Well-based screening procedure to perform activity testing and epitope mapping for filamentous phage-displayed scFvs in an integrated manner with a single assay.",

keywords = "Antibody discovery, Antibody phage library, Array-in-well (AinW), Epitope mapping, Peptide array, Protein array",

author = "Urpo Lamminm{\"a}ki and Gaurav Batra and Petri Saviranta",

year = "2018",

month = jan,

day = "1",

doi = "10.1007/978-1-4939-7841-0_9",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Springer",

pages = "129--140",

booktitle = "Epitope Mapping Protocols",

address = "Germany",

}

TY - CHAP

T1 - Array-in-well epitope mapping of phage-displayed antibodies

AU - Lamminmäki, Urpo

AU - Batra, Gaurav

AU - Saviranta, Petri

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Novel affinity reagents, such as single chain (scFv) antibody fragments, can be generated by isolating them from recombinant protein libraries using phage display selection. A successful selection process against a target protein can produce a number of binder candidates among which the desired binders are identified by screening and characterization of individual clones. Obtaining information on the binding properties, such as the binding epitope, already during the screening step helps to choose the most useful candidates for further development at early phase saving time and resources. To this end, we describe here an Array-in-Well-based screening procedure to perform activity testing and epitope mapping for filamentous phage-displayed scFvs in an integrated manner with a single assay.

AB - Novel affinity reagents, such as single chain (scFv) antibody fragments, can be generated by isolating them from recombinant protein libraries using phage display selection. A successful selection process against a target protein can produce a number of binder candidates among which the desired binders are identified by screening and characterization of individual clones. Obtaining information on the binding properties, such as the binding epitope, already during the screening step helps to choose the most useful candidates for further development at early phase saving time and resources. To this end, we describe here an Array-in-Well-based screening procedure to perform activity testing and epitope mapping for filamentous phage-displayed scFvs in an integrated manner with a single assay.

KW - Antibody discovery

KW - Antibody phage library

KW - Array-in-well (AinW)

KW - Epitope mapping

KW - Peptide array

KW - Protein array

UR - http://www.scopus.com/inward/record.url?scp=85046358819&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7841-0_9

DO - 10.1007/978-1-4939-7841-0_9

M3 - Chapter or book article

AN - SCOPUS:85046358819

T3 - Methods in Molecular Biology

SP - 129

EP - 140

BT - Epitope Mapping Protocols

PB - Springer

ER -

Array-in-well epitope mapping of phage-displayed antibodies

Abstract

Publication series

Keywords

Access to Document

Other files and links

Fingerprint

Cite this