Array-in-well epitope mapping of phage-displayed antibodies

Urpo Lamminmäki, Gaurav Batra, Petri Saviranta

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

Abstract

Novel affinity reagents, such as single chain (scFv) antibody fragments, can be generated by isolating them from recombinant protein libraries using phage display selection. A successful selection process against a target protein can produce a number of binder candidates among which the desired binders are identified by screening and characterization of individual clones. Obtaining information on the binding properties, such as the binding epitope, already during the screening step helps to choose the most useful candidates for further development at early phase saving time and resources. To this end, we describe here an Array-in-Well-based screening procedure to perform activity testing and epitope mapping for filamentous phage-displayed scFvs in an integrated manner with a single assay.

Original languageEnglish
Title of host publicationEpitope Mapping Protocols
PublisherSpringer
Pages129-140
Number of pages12
DOIs
Publication statusPublished - 1 Jan 2018
MoE publication typeA3 Part of a book or another research book

Publication series

SeriesMethods in Molecular Biology
Volume1785
ISSN1064-3745

Keywords

  • Antibody discovery
  • Antibody phage library
  • Array-in-well (AinW)
  • Epitope mapping
  • Peptide array
  • Protein array

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  • Cite this

    Lamminmäki, U., Batra, G., & Saviranta, P. (2018). Array-in-well epitope mapping of phage-displayed antibodies. In Epitope Mapping Protocols (pp. 129-140). Springer. Methods in Molecular Biology, Vol.. 1785 https://doi.org/10.1007/978-1-4939-7841-0_9