Array-in-well epitope mapping of phage-displayed antibodies

Urpo Lamminmäki, Gaurav Batra, Petri Saviranta

    Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

    Abstract

    Novel affinity reagents, such as single chain (scFv) antibody fragments, can be generated by isolating them from recombinant protein libraries using phage display selection. A successful selection process against a target protein can produce a number of binder candidates among which the desired binders are identified by screening and characterization of individual clones. Obtaining information on the binding properties, such as the binding epitope, already during the screening step helps to choose the most useful candidates for further development at early phase saving time and resources. To this end, we describe here an Array-in-Well-based screening procedure to perform activity testing and epitope mapping for filamentous phage-displayed scFvs in an integrated manner with a single assay.

    Original languageEnglish
    Title of host publicationEpitope Mapping Protocols
    PublisherSpringer
    Pages129-140
    Number of pages12
    DOIs
    Publication statusPublished - 1 Jan 2018
    MoE publication typeA3 Part of a book or another research book

    Publication series

    SeriesMethods in Molecular Biology
    Volume1785
    ISSN1064-3745

    Keywords

    • Antibody discovery
    • Antibody phage library
    • Array-in-well (AinW)
    • Epitope mapping
    • Peptide array
    • Protein array

    Fingerprint Dive into the research topics of 'Array-in-well epitope mapping of phage-displayed antibodies'. Together they form a unique fingerprint.

    Cite this