Array-in-well platform-based multiplex assay for the simultaneous detection of anti-HIV- and treponemal-antibodies, and Hepatitis B surface antigen

Sheikh Mohd Talha (Corresponding Author), Petri Saviranta, Liisa Hattara, Tytti Vuorinen, Jukka Hytönen, Navin C. Khanna, Kim Pettersson

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Multiplex assays detecting sets of related clinical analytes simultaneously can save considerable amount of time and resources. Array-in-well (AIW) is a powerful platform for the multiplex detection of different analytes where microarrays can be printed at the bottom of microtiter wells, thus combining the potential of microarrays with the ease of handling microtiter wells. We have developed a single-step AIW assay for the simultaneous screening of HIV, Treponema pallidum subspecies pallidum (causing syphilis) and Hepatitis B virus infections targeting the specific detection of anti-HIV- and treponemal-antibodies and Hepatitis B surface antigen (HBsAg), respectively, using two different fluorescent label technologies i.e. DyLight 633 and europium nanoparticle. Double-antigen assay formats were used for anti-HIV- and treponemal-antibody detection that can simultaneously detect both IgG and IgM, and thus reduce the window period of detection. AIW assay was evaluated with well characterized serum/plasma samples (n = 111), and the qualitative results were in near complete agreement with those of the reference assays. The AIW assay exhibited 100% sensitivities for all three analytes, and 100% specificities for anti-HIV antibodies and HBsAg, and 98.6% specificity for treponemal antibodies. The limit of detection of HBsAg in AIW assay was 0.18. ng/ml. This high performing AIW assay has the potential to be used as a multiplex screening test for these three infections.
Original languageEnglish
Pages (from-to)21-27
JournalJournal of Immunological Methods
Volume429
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

HIV Antibodies
Hepatitis B Surface Antigens
Europium
Treponema pallidum
Globus Pallidus
Antibody Specificity
Virus Diseases
Syphilis
Hepatitis B virus
Nanoparticles
Immunoglobulin M
Limit of Detection
Anti-Idiotypic Antibodies
Immunoglobulin G
HIV
Technology
Antigens
Infection
Serum

Keywords

  • array-in-well
  • diagnostics
  • hepatitis B virus
  • HIV
  • multiplex
  • syphilis

Cite this

Talha, Sheikh Mohd ; Saviranta, Petri ; Hattara, Liisa ; Vuorinen, Tytti ; Hytönen, Jukka ; Khanna, Navin C. ; Pettersson, Kim. / Array-in-well platform-based multiplex assay for the simultaneous detection of anti-HIV- and treponemal-antibodies, and Hepatitis B surface antigen. In: Journal of Immunological Methods. 2016 ; Vol. 429. pp. 21-27.
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title = "Array-in-well platform-based multiplex assay for the simultaneous detection of anti-HIV- and treponemal-antibodies, and Hepatitis B surface antigen",
abstract = "Multiplex assays detecting sets of related clinical analytes simultaneously can save considerable amount of time and resources. Array-in-well (AIW) is a powerful platform for the multiplex detection of different analytes where microarrays can be printed at the bottom of microtiter wells, thus combining the potential of microarrays with the ease of handling microtiter wells. We have developed a single-step AIW assay for the simultaneous screening of HIV, Treponema pallidum subspecies pallidum (causing syphilis) and Hepatitis B virus infections targeting the specific detection of anti-HIV- and treponemal-antibodies and Hepatitis B surface antigen (HBsAg), respectively, using two different fluorescent label technologies i.e. DyLight 633 and europium nanoparticle. Double-antigen assay formats were used for anti-HIV- and treponemal-antibody detection that can simultaneously detect both IgG and IgM, and thus reduce the window period of detection. AIW assay was evaluated with well characterized serum/plasma samples (n = 111), and the qualitative results were in near complete agreement with those of the reference assays. The AIW assay exhibited 100{\%} sensitivities for all three analytes, and 100{\%} specificities for anti-HIV antibodies and HBsAg, and 98.6{\%} specificity for treponemal antibodies. The limit of detection of HBsAg in AIW assay was 0.18. ng/ml. This high performing AIW assay has the potential to be used as a multiplex screening test for these three infections.",
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Array-in-well platform-based multiplex assay for the simultaneous detection of anti-HIV- and treponemal-antibodies, and Hepatitis B surface antigen. / Talha, Sheikh Mohd (Corresponding Author); Saviranta, Petri; Hattara, Liisa; Vuorinen, Tytti; Hytönen, Jukka; Khanna, Navin C.; Pettersson, Kim.

In: Journal of Immunological Methods, Vol. 429, 2016, p. 21-27.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Array-in-well platform-based multiplex assay for the simultaneous detection of anti-HIV- and treponemal-antibodies, and Hepatitis B surface antigen

AU - Talha, Sheikh Mohd

AU - Saviranta, Petri

AU - Hattara, Liisa

AU - Vuorinen, Tytti

AU - Hytönen, Jukka

AU - Khanna, Navin C.

AU - Pettersson, Kim

PY - 2016

Y1 - 2016

N2 - Multiplex assays detecting sets of related clinical analytes simultaneously can save considerable amount of time and resources. Array-in-well (AIW) is a powerful platform for the multiplex detection of different analytes where microarrays can be printed at the bottom of microtiter wells, thus combining the potential of microarrays with the ease of handling microtiter wells. We have developed a single-step AIW assay for the simultaneous screening of HIV, Treponema pallidum subspecies pallidum (causing syphilis) and Hepatitis B virus infections targeting the specific detection of anti-HIV- and treponemal-antibodies and Hepatitis B surface antigen (HBsAg), respectively, using two different fluorescent label technologies i.e. DyLight 633 and europium nanoparticle. Double-antigen assay formats were used for anti-HIV- and treponemal-antibody detection that can simultaneously detect both IgG and IgM, and thus reduce the window period of detection. AIW assay was evaluated with well characterized serum/plasma samples (n = 111), and the qualitative results were in near complete agreement with those of the reference assays. The AIW assay exhibited 100% sensitivities for all three analytes, and 100% specificities for anti-HIV antibodies and HBsAg, and 98.6% specificity for treponemal antibodies. The limit of detection of HBsAg in AIW assay was 0.18. ng/ml. This high performing AIW assay has the potential to be used as a multiplex screening test for these three infections.

AB - Multiplex assays detecting sets of related clinical analytes simultaneously can save considerable amount of time and resources. Array-in-well (AIW) is a powerful platform for the multiplex detection of different analytes where microarrays can be printed at the bottom of microtiter wells, thus combining the potential of microarrays with the ease of handling microtiter wells. We have developed a single-step AIW assay for the simultaneous screening of HIV, Treponema pallidum subspecies pallidum (causing syphilis) and Hepatitis B virus infections targeting the specific detection of anti-HIV- and treponemal-antibodies and Hepatitis B surface antigen (HBsAg), respectively, using two different fluorescent label technologies i.e. DyLight 633 and europium nanoparticle. Double-antigen assay formats were used for anti-HIV- and treponemal-antibody detection that can simultaneously detect both IgG and IgM, and thus reduce the window period of detection. AIW assay was evaluated with well characterized serum/plasma samples (n = 111), and the qualitative results were in near complete agreement with those of the reference assays. The AIW assay exhibited 100% sensitivities for all three analytes, and 100% specificities for anti-HIV antibodies and HBsAg, and 98.6% specificity for treponemal antibodies. The limit of detection of HBsAg in AIW assay was 0.18. ng/ml. This high performing AIW assay has the potential to be used as a multiplex screening test for these three infections.

KW - array-in-well

KW - diagnostics

KW - hepatitis B virus

KW - HIV

KW - multiplex

KW - syphilis

U2 - 10.1016/j.jim.2015.12.007

DO - 10.1016/j.jim.2015.12.007

M3 - Article

VL - 429

SP - 21

EP - 27

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

ER -