Abstract
The possible use of specific biochemical compounds
identified in wheat grains was evaluated for monitoring
barley grain tissues during fractionation. First barley
grain anatomy was studied through microscopic observation
and quantification of the relative proportion of each
anatomical part in four distinct barley samples from both
hulled and hulless genotypes. As expected from cereal
phylogeny and irrespective of the possible presence of
hull, common features were observed between barley and
wheat grains, but the aleurone layer predominated in the
outer layers. The specific location of the compounds
identified in wheat was established. Phytic acid was
specifically localized in the aleurone layer and
alkylresorcinols in the composite layer containing the
testa, even if their concentration differed from that
observed in wheat grain tissues. Thus, these two markers
identified in wheat can be used to monitor the
corresponding barley tissues, independent of the presence
of hulls. Conversely, phenolic compounds, either ferulic
acid trimer or p-coumaric acid, cannot be used to monitor
respectively the outer pericarp or the aleurone cell
walls in barley grains. p-coumaric acid was identified as
an efficient marker of the hull and could be used to
distinguish hulled or hulless barley grains and to help
monitor the dehulling process.
Original language | English |
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Pages (from-to) | 11-18 |
Number of pages | 8 |
Journal | Journal of Cereal Science |
Volume | 74 |
DOIs | |
Publication status | Published - 2017 |
MoE publication type | A1 Journal article-refereed |
Keywords
- aleurone
- barley
- cereal kernel
- histology
- hulls
- microscopy