Assessment of identity of filamentous fungi colonizing water-damaged building materials

X. Lian, M. Lackner, G. S. de Hoog, A. H. G. Gerrits van de Ende, Outi Priha, Maija-Liisa Suihko, J. Houbraken, J. Varga, R. A. Samson, B. Mälarstig, P. Thompson, R. Stott, M. D. Richardson

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Growth of moulds in moisture-damaged buildings has various adverse health effects, such as allergic diseases. To protect the inhabitants being harmed by moulds or their toxins, indoor microbiota need to be investigated; therefore an efficient identification method is needed. To assess the diversity of fungi colonizing temporarily damp indoor building materials in northern European countries (Finland, Sweden, Denmark, and U.K.), 62 samples from 22 houses were taken. In total 234 pure cultures were acquired and identified using morphological and molecular techniques (sequence comparison of rDNA internal transcribed spacer and BT2 [partial b-tubulin]). Comparing CBS-database with GenBank by blasting ITS and BT2 sequences we found correct identification down to species level by GenBank in only 12.8 % and 16.0 %, respectively. GenBank is an inadequate tool for reliable identification of less common fungi. Final identification of fungi is based on internal databases at CBS validated by ex-type strains. Within all samples we identified strains affiliated to 43 species belonging to 20 genera. Predominant were oligotrophic fungi such as Penicillium, Aspergillus and Cladosporium; some may serve as indicator organisms for indoor moisture problems. Based on final identifications, species-specific probes were developed for fast identification of moulds in indoor environments.
Original languageEnglish
Pages (from-to)49-66
JournalSydowia
Volume63
Issue number1
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

fungus
molds (fungi)
fungi
water
moisture
identification method
Cladosporium
Northern European region
tubulin
Penicillium
blasting
Aspergillus
Denmark
probes (equipment)
toxin
internal transcribed spacers
indicator species
Finland
Sweden
toxins

Keywords

  • Indoor fungi
  • molecular identification
  • species-specific probes

Cite this

Lian, X., Lackner, M., de Hoog, G. S., Gerrits van de Ende, A. H. G., Priha, O., Suihko, M-L., ... Richardson, M. D. (2011). Assessment of identity of filamentous fungi colonizing water-damaged building materials. Sydowia, 63(1), 49-66.
Lian, X. ; Lackner, M. ; de Hoog, G. S. ; Gerrits van de Ende, A. H. G. ; Priha, Outi ; Suihko, Maija-Liisa ; Houbraken, J. ; Varga, J. ; Samson, R. A. ; Mälarstig, B. ; Thompson, P. ; Stott, R. ; Richardson, M. D. / Assessment of identity of filamentous fungi colonizing water-damaged building materials. In: Sydowia. 2011 ; Vol. 63, No. 1. pp. 49-66.
@article{84b6513e3c944a4d9c26fbeb543a9c71,
title = "Assessment of identity of filamentous fungi colonizing water-damaged building materials",
abstract = "Growth of moulds in moisture-damaged buildings has various adverse health effects, such as allergic diseases. To protect the inhabitants being harmed by moulds or their toxins, indoor microbiota need to be investigated; therefore an efficient identification method is needed. To assess the diversity of fungi colonizing temporarily damp indoor building materials in northern European countries (Finland, Sweden, Denmark, and U.K.), 62 samples from 22 houses were taken. In total 234 pure cultures were acquired and identified using morphological and molecular techniques (sequence comparison of rDNA internal transcribed spacer and BT2 [partial b-tubulin]). Comparing CBS-database with GenBank by blasting ITS and BT2 sequences we found correct identification down to species level by GenBank in only 12.8 {\%} and 16.0 {\%}, respectively. GenBank is an inadequate tool for reliable identification of less common fungi. Final identification of fungi is based on internal databases at CBS validated by ex-type strains. Within all samples we identified strains affiliated to 43 species belonging to 20 genera. Predominant were oligotrophic fungi such as Penicillium, Aspergillus and Cladosporium; some may serve as indicator organisms for indoor moisture problems. Based on final identifications, species-specific probes were developed for fast identification of moulds in indoor environments.",
keywords = "Indoor fungi, molecular identification, species-specific probes",
author = "X. Lian and M. Lackner and {de Hoog}, {G. S.} and {Gerrits van de Ende}, {A. H. G.} and Outi Priha and Maija-Liisa Suihko and J. Houbraken and J. Varga and Samson, {R. A.} and B. M{\"a}larstig and P. Thompson and R. Stott and Richardson, {M. D.}",
year = "2011",
language = "English",
volume = "63",
pages = "49--66",
journal = "Sydowia",
issn = "0082-0598",
publisher = "Verlag Ferdinand Berger und Sohne GmbH",
number = "1",

}

Lian, X, Lackner, M, de Hoog, GS, Gerrits van de Ende, AHG, Priha, O, Suihko, M-L, Houbraken, J, Varga, J, Samson, RA, Mälarstig, B, Thompson, P, Stott, R & Richardson, MD 2011, 'Assessment of identity of filamentous fungi colonizing water-damaged building materials', Sydowia, vol. 63, no. 1, pp. 49-66.

Assessment of identity of filamentous fungi colonizing water-damaged building materials. / Lian, X.; Lackner, M.; de Hoog, G. S.; Gerrits van de Ende, A. H. G.; Priha, Outi; Suihko, Maija-Liisa; Houbraken, J.; Varga, J.; Samson, R. A.; Mälarstig, B.; Thompson, P.; Stott, R.; Richardson, M. D.

In: Sydowia, Vol. 63, No. 1, 2011, p. 49-66.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Assessment of identity of filamentous fungi colonizing water-damaged building materials

AU - Lian, X.

AU - Lackner, M.

AU - de Hoog, G. S.

AU - Gerrits van de Ende, A. H. G.

AU - Priha, Outi

AU - Suihko, Maija-Liisa

AU - Houbraken, J.

AU - Varga, J.

AU - Samson, R. A.

AU - Mälarstig, B.

AU - Thompson, P.

AU - Stott, R.

AU - Richardson, M. D.

PY - 2011

Y1 - 2011

N2 - Growth of moulds in moisture-damaged buildings has various adverse health effects, such as allergic diseases. To protect the inhabitants being harmed by moulds or their toxins, indoor microbiota need to be investigated; therefore an efficient identification method is needed. To assess the diversity of fungi colonizing temporarily damp indoor building materials in northern European countries (Finland, Sweden, Denmark, and U.K.), 62 samples from 22 houses were taken. In total 234 pure cultures were acquired and identified using morphological and molecular techniques (sequence comparison of rDNA internal transcribed spacer and BT2 [partial b-tubulin]). Comparing CBS-database with GenBank by blasting ITS and BT2 sequences we found correct identification down to species level by GenBank in only 12.8 % and 16.0 %, respectively. GenBank is an inadequate tool for reliable identification of less common fungi. Final identification of fungi is based on internal databases at CBS validated by ex-type strains. Within all samples we identified strains affiliated to 43 species belonging to 20 genera. Predominant were oligotrophic fungi such as Penicillium, Aspergillus and Cladosporium; some may serve as indicator organisms for indoor moisture problems. Based on final identifications, species-specific probes were developed for fast identification of moulds in indoor environments.

AB - Growth of moulds in moisture-damaged buildings has various adverse health effects, such as allergic diseases. To protect the inhabitants being harmed by moulds or their toxins, indoor microbiota need to be investigated; therefore an efficient identification method is needed. To assess the diversity of fungi colonizing temporarily damp indoor building materials in northern European countries (Finland, Sweden, Denmark, and U.K.), 62 samples from 22 houses were taken. In total 234 pure cultures were acquired and identified using morphological and molecular techniques (sequence comparison of rDNA internal transcribed spacer and BT2 [partial b-tubulin]). Comparing CBS-database with GenBank by blasting ITS and BT2 sequences we found correct identification down to species level by GenBank in only 12.8 % and 16.0 %, respectively. GenBank is an inadequate tool for reliable identification of less common fungi. Final identification of fungi is based on internal databases at CBS validated by ex-type strains. Within all samples we identified strains affiliated to 43 species belonging to 20 genera. Predominant were oligotrophic fungi such as Penicillium, Aspergillus and Cladosporium; some may serve as indicator organisms for indoor moisture problems. Based on final identifications, species-specific probes were developed for fast identification of moulds in indoor environments.

KW - Indoor fungi

KW - molecular identification

KW - species-specific probes

M3 - Article

VL - 63

SP - 49

EP - 66

JO - Sydowia

JF - Sydowia

SN - 0082-0598

IS - 1

ER -

Lian X, Lackner M, de Hoog GS, Gerrits van de Ende AHG, Priha O, Suihko M-L et al. Assessment of identity of filamentous fungi colonizing water-damaged building materials. Sydowia. 2011;63(1):49-66.