Abstract
The activity profile of a 1:0.30 mixture of Celluclast 1.5 L FG and
Novozym 188 (Novozymes) was investigated using Whatman #1 filter paper (W1FP)
as a single substrate for hydrolysis. The procedure was based on the
ability of the enzymes to release total (RSTot), insoluble (RSInsol) and
soluble (RSSol) reducing sugars from W1FP. RSInsol was used to estimate
endoglucanase (EnG) activity whereas exoglucanases (ExG) were assessed by
measuring RSSol in the presence of d-gluconolactone. Finally, the
ß-glucosidase (ßG) activity was derived from the difference between RSSol
measurements in the presence and absence of d-gluconolactone. When this
analytical procedure was applied to W1FP using 9.64 mg mL-1 of the enzyme
mixture, the relative contributions of EnG, ExG and ßG to the total cellulase
activity were 63.28%, 12.02% and 24.70%, respectively. Also, this ratio
changed with changes in the enzyme loading, giving a new insight into the
synergy that exists among the enzymes.
Original language | English |
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Pages (from-to) | 392-396 |
Journal | Bioresource Technology |
Volume | 151 |
DOIs | |
Publication status | Published - 2014 |
MoE publication type | A1 Journal article-refereed |
Keywords
- cellulases
- enzyme activity
- filter paper
- reducing sugars
- synergism