Association of lipidome remodeling in the adipocyte membrane with acquired obesity in humans

K. H. Pietiläinen, T. Róg, Tuulikki Seppänen-Laakso, S. Virtue, Peddinti Gopalacharyulu, Jing Tang, S. Rodriguez-Cuenca, A. Maciejewski, J. Naukkarinen, Anna-Liisa Ruskeepää, Perttu Niemelä, Laxman Yetukuri, C. Y. Tan, Vidya Velagapudi, Sandra Castillo, Heli Nygren, Tuulia Hyötyläinen, A. Rissanen, J. Kaprio, H. Yki-Järvinen & 3 others I. Vattulainen, A. Vidal-Puig, Matej Orešič

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131 Citations (Scopus)

Abstract

Identification of early mechanisms that may lead from obesity towards complications such as metabolic syndrome is of great interest. Here we performed lipidomic analyses of adipose tissue in twin pairs discordant for obesity but still metabolically compensated. In parallel we studied more evolved states of obesity by investigating a separated set of individuals considered to be morbidly obese. Despite lower dietary polyunsaturated fatty acid intake, the obese twin individuals had increased proportions of palmitoleic and arachidonic acids in their adipose tissue, including increased levels of ethanolamine plasmalogens containing arachidonic acid. Information gathered from these experimental groups was used for molecular dynamics simulations of lipid bilayers combined with dependency network analysis of combined clinical, lipidomics, and gene expression data. The simulations suggested that the observed lipid remodeling maintains the biophysical properties of lipid membranes, at the price, however, of increasing their vulnerability to inflammation. Conversely, in morbidly obese subjects, the proportion of plasmalogens containing arachidonic acid in the adipose tissue was markedly decreased. We also show by in vitro Elovl6 knockdown that the lipid network regulating the observed remodeling may be amenable to genetic modulation. Together, our novel approach suggests a physiological mechanism by which adaptation of adipocyte membranes to adipose tissue expansion associates with positive energy balance, potentially leading to higher vulnerability to inflammation in acquired obesity. Further studies will be needed to determine the cause of this effect.
Original languageEnglish
Article numbere1000623
Number of pages14
JournalPLoS Biology
Volume9
Issue number6
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

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adipocytes
Adipocytes
adipose tissue
Adipose Tissue
obesity
Obesity
arachidonic acid
Tissue
Membranes
Arachidonic Acid
lipids
inflammation
Plasmalogens
Tissue Expansion
Arachidonic Acids
Inflammation
Lipids
palmitoleic acid
lipid bilayers
Lipid bilayers

Cite this

Pietiläinen, K. H., Róg, T., Seppänen-Laakso, T., Virtue, S., Gopalacharyulu, P., Tang, J., ... Orešič, M. (2011). Association of lipidome remodeling in the adipocyte membrane with acquired obesity in humans. PLoS Biology, 9(6), [e1000623]. https://doi.org/10.1371/journal.pbio.1000623
Pietiläinen, K. H. ; Róg, T. ; Seppänen-Laakso, Tuulikki ; Virtue, S. ; Gopalacharyulu, Peddinti ; Tang, Jing ; Rodriguez-Cuenca, S. ; Maciejewski, A. ; Naukkarinen, J. ; Ruskeepää, Anna-Liisa ; Niemelä, Perttu ; Yetukuri, Laxman ; Tan, C. Y. ; Velagapudi, Vidya ; Castillo, Sandra ; Nygren, Heli ; Hyötyläinen, Tuulia ; Rissanen, A. ; Kaprio, J. ; Yki-Järvinen, H. ; Vattulainen, I. ; Vidal-Puig, A. ; Orešič, Matej. / Association of lipidome remodeling in the adipocyte membrane with acquired obesity in humans. In: PLoS Biology. 2011 ; Vol. 9, No. 6.
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Pietiläinen, KH, Róg, T, Seppänen-Laakso, T, Virtue, S, Gopalacharyulu, P, Tang, J, Rodriguez-Cuenca, S, Maciejewski, A, Naukkarinen, J, Ruskeepää, A-L, Niemelä, P, Yetukuri, L, Tan, CY, Velagapudi, V, Castillo, S, Nygren, H, Hyötyläinen, T, Rissanen, A, Kaprio, J, Yki-Järvinen, H, Vattulainen, I, Vidal-Puig, A & Orešič, M 2011, 'Association of lipidome remodeling in the adipocyte membrane with acquired obesity in humans', PLoS Biology, vol. 9, no. 6, e1000623. https://doi.org/10.1371/journal.pbio.1000623

Association of lipidome remodeling in the adipocyte membrane with acquired obesity in humans. / Pietiläinen, K. H.; Róg, T.; Seppänen-Laakso, Tuulikki; Virtue, S.; Gopalacharyulu, Peddinti; Tang, Jing; Rodriguez-Cuenca, S.; Maciejewski, A.; Naukkarinen, J.; Ruskeepää, Anna-Liisa; Niemelä, Perttu; Yetukuri, Laxman; Tan, C. Y.; Velagapudi, Vidya; Castillo, Sandra; Nygren, Heli; Hyötyläinen, Tuulia; Rissanen, A.; Kaprio, J.; Yki-Järvinen, H.; Vattulainen, I.; Vidal-Puig, A.; Orešič, Matej (Corresponding Author).

In: PLoS Biology, Vol. 9, No. 6, e1000623, 2011.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Seppänen-Laakso, Tuulikki

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AU - Gopalacharyulu, Peddinti

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AU - Maciejewski, A.

AU - Naukkarinen, J.

AU - Ruskeepää, Anna-Liisa

AU - Niemelä, Perttu

AU - Yetukuri, Laxman

AU - Tan, C. Y.

AU - Velagapudi, Vidya

AU - Castillo, Sandra

AU - Nygren, Heli

AU - Hyötyläinen, Tuulia

AU - Rissanen, A.

AU - Kaprio, J.

AU - Yki-Järvinen, H.

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AU - Vidal-Puig, A.

AU - Orešič, Matej

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