Association of sequence variations in the gene encoding insulin-like growth factor binding protein 5 with adiponectin

P. Kallio (Corresponding Author), A.-M. Tolppanen, M. Kolehmainen, Kaisa Poutanen, J. Lindström, J. Tuomilehto, T. Kuulasmaa, J. Kuusisto, L. Pulkkinen, M. Uusitupa

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Abstract

Background:

Insulin-like growth factor binding protein 5 (IGFBP5) binds to IGF and thus modulates IGF signaling pathway. We have shown earlier that the IGFBP5 gene was downregulated in the adipose tissue after 12-week carbohydrate diet with low insulinemic response.

Objective:

The aim was to examine the putative contribution of genetic variation of the IGFBP5 gene to the characteristics of metabolic syndrome and incidence of type 2 diabetes (T2DM) in the Finnish Diabetes Prevention Study (DPS).

Methods:

DPS is a longitudinal study where 522 subjects with impaired glucose tolerance were randomized to either lifestyle intervention group or control group. DNA was available from 507 subjects (mean body mass index (BMI) 31.2±4.5 kg/m2, age 55±7 years). The eight single-nucleotide polymorphisms (SNPs) were selected from HapMap database and genotyped by Taqman allelic discrimination protocol. The main results were confirmed in a larger cross-sectional study population (METSIM). In addition, the gene expression of IGFBP5 was studied in two previously published study populations (FUNGENUT and GENOBIN) of 124 subjects with insulin resistance (BMI 32.2±3.5 kg/m2, age 57.7±7.4 years).

Results:

Three out of eight IGFBP5 markers (rs9341234, rs3276 and rs11575134) were significantly associated with circulating adiponectin concentrations in men. Furthermore, mRNA expression studies of subcutaneous adipose tissue showed that mRNA concentrations of IGFBP5 correlated with adiponectin concentrations in all subjects and in women. None of the IGFBP5 SNPs were associated with T2DM.

Conclusions:

Our findings show that IGFBP5 has a gender-specific association with adiponectin, which may modulate the development of metabolic syndrome.

Original languageEnglish
Pages (from-to)80-88
Number of pages9
JournalInternational Journal of Obesity
Volume33
Issue number1
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Insulin-Like Growth Factor Binding Protein 5
Adiponectin
Genes
Single Nucleotide Polymorphism
Body Mass Index
Carbohydrate-Restricted Diet
HapMap Project
Messenger RNA
Glucose Intolerance
Subcutaneous Fat
Type 2 Diabetes Mellitus
Population
Longitudinal Studies
Insulin Resistance
Adipose Tissue
Life Style
Down-Regulation
Cross-Sectional Studies
Databases
Gene Expression

Cite this

Kallio, P. ; Tolppanen, A.-M. ; Kolehmainen, M. ; Poutanen, Kaisa ; Lindström, J. ; Tuomilehto, J. ; Kuulasmaa, T. ; Kuusisto, J. ; Pulkkinen, L. ; Uusitupa, M. / Association of sequence variations in the gene encoding insulin-like growth factor binding protein 5 with adiponectin. In: International Journal of Obesity. 2009 ; Vol. 33, No. 1. pp. 80-88.
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abstract = "Background:Insulin-like growth factor binding protein 5 (IGFBP5) binds to IGF and thus modulates IGF signaling pathway. We have shown earlier that the IGFBP5 gene was downregulated in the adipose tissue after 12-week carbohydrate diet with low insulinemic response.Objective:The aim was to examine the putative contribution of genetic variation of the IGFBP5 gene to the characteristics of metabolic syndrome and incidence of type 2 diabetes (T2DM) in the Finnish Diabetes Prevention Study (DPS).Methods:DPS is a longitudinal study where 522 subjects with impaired glucose tolerance were randomized to either lifestyle intervention group or control group. DNA was available from 507 subjects (mean body mass index (BMI) 31.2±4.5 kg/m2, age 55±7 years). The eight single-nucleotide polymorphisms (SNPs) were selected from HapMap database and genotyped by Taqman allelic discrimination protocol. The main results were confirmed in a larger cross-sectional study population (METSIM). In addition, the gene expression of IGFBP5 was studied in two previously published study populations (FUNGENUT and GENOBIN) of 124 subjects with insulin resistance (BMI 32.2±3.5 kg/m2, age 57.7±7.4 years).Results:Three out of eight IGFBP5 markers (rs9341234, rs3276 and rs11575134) were significantly associated with circulating adiponectin concentrations in men. Furthermore, mRNA expression studies of subcutaneous adipose tissue showed that mRNA concentrations of IGFBP5 correlated with adiponectin concentrations in all subjects and in women. None of the IGFBP5 SNPs were associated with T2DM.Conclusions:Our findings show that IGFBP5 has a gender-specific association with adiponectin, which may modulate the development of metabolic syndrome.",
author = "P. Kallio and A.-M. Tolppanen and M. Kolehmainen and Kaisa Poutanen and J. Lindstr{\"o}m and J. Tuomilehto and T. Kuulasmaa and J. Kuusisto and L. Pulkkinen and M. Uusitupa",
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Kallio, P, Tolppanen, A-M, Kolehmainen, M, Poutanen, K, Lindström, J, Tuomilehto, J, Kuulasmaa, T, Kuusisto, J, Pulkkinen, L & Uusitupa, M 2009, 'Association of sequence variations in the gene encoding insulin-like growth factor binding protein 5 with adiponectin', International Journal of Obesity, vol. 33, no. 1, pp. 80-88. https://doi.org/10.1038/ijo.2008.196

Association of sequence variations in the gene encoding insulin-like growth factor binding protein 5 with adiponectin. / Kallio, P. (Corresponding Author); Tolppanen, A.-M.; Kolehmainen, M.; Poutanen, Kaisa; Lindström, J.; Tuomilehto, J.; Kuulasmaa, T.; Kuusisto, J.; Pulkkinen, L.; Uusitupa, M.

In: International Journal of Obesity, Vol. 33, No. 1, 2009, p. 80-88.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Association of sequence variations in the gene encoding insulin-like growth factor binding protein 5 with adiponectin

AU - Kallio, P.

AU - Tolppanen, A.-M.

AU - Kolehmainen, M.

AU - Poutanen, Kaisa

AU - Lindström, J.

AU - Tuomilehto, J.

AU - Kuulasmaa, T.

AU - Kuusisto, J.

AU - Pulkkinen, L.

AU - Uusitupa, M.

PY - 2009

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N2 - Background:Insulin-like growth factor binding protein 5 (IGFBP5) binds to IGF and thus modulates IGF signaling pathway. We have shown earlier that the IGFBP5 gene was downregulated in the adipose tissue after 12-week carbohydrate diet with low insulinemic response.Objective:The aim was to examine the putative contribution of genetic variation of the IGFBP5 gene to the characteristics of metabolic syndrome and incidence of type 2 diabetes (T2DM) in the Finnish Diabetes Prevention Study (DPS).Methods:DPS is a longitudinal study where 522 subjects with impaired glucose tolerance were randomized to either lifestyle intervention group or control group. DNA was available from 507 subjects (mean body mass index (BMI) 31.2±4.5 kg/m2, age 55±7 years). The eight single-nucleotide polymorphisms (SNPs) were selected from HapMap database and genotyped by Taqman allelic discrimination protocol. The main results were confirmed in a larger cross-sectional study population (METSIM). In addition, the gene expression of IGFBP5 was studied in two previously published study populations (FUNGENUT and GENOBIN) of 124 subjects with insulin resistance (BMI 32.2±3.5 kg/m2, age 57.7±7.4 years).Results:Three out of eight IGFBP5 markers (rs9341234, rs3276 and rs11575134) were significantly associated with circulating adiponectin concentrations in men. Furthermore, mRNA expression studies of subcutaneous adipose tissue showed that mRNA concentrations of IGFBP5 correlated with adiponectin concentrations in all subjects and in women. None of the IGFBP5 SNPs were associated with T2DM.Conclusions:Our findings show that IGFBP5 has a gender-specific association with adiponectin, which may modulate the development of metabolic syndrome.

AB - Background:Insulin-like growth factor binding protein 5 (IGFBP5) binds to IGF and thus modulates IGF signaling pathway. We have shown earlier that the IGFBP5 gene was downregulated in the adipose tissue after 12-week carbohydrate diet with low insulinemic response.Objective:The aim was to examine the putative contribution of genetic variation of the IGFBP5 gene to the characteristics of metabolic syndrome and incidence of type 2 diabetes (T2DM) in the Finnish Diabetes Prevention Study (DPS).Methods:DPS is a longitudinal study where 522 subjects with impaired glucose tolerance were randomized to either lifestyle intervention group or control group. DNA was available from 507 subjects (mean body mass index (BMI) 31.2±4.5 kg/m2, age 55±7 years). The eight single-nucleotide polymorphisms (SNPs) were selected from HapMap database and genotyped by Taqman allelic discrimination protocol. The main results were confirmed in a larger cross-sectional study population (METSIM). In addition, the gene expression of IGFBP5 was studied in two previously published study populations (FUNGENUT and GENOBIN) of 124 subjects with insulin resistance (BMI 32.2±3.5 kg/m2, age 57.7±7.4 years).Results:Three out of eight IGFBP5 markers (rs9341234, rs3276 and rs11575134) were significantly associated with circulating adiponectin concentrations in men. Furthermore, mRNA expression studies of subcutaneous adipose tissue showed that mRNA concentrations of IGFBP5 correlated with adiponectin concentrations in all subjects and in women. None of the IGFBP5 SNPs were associated with T2DM.Conclusions:Our findings show that IGFBP5 has a gender-specific association with adiponectin, which may modulate the development of metabolic syndrome.

U2 - 10.1038/ijo.2008.196

DO - 10.1038/ijo.2008.196

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JO - International Journal of Obesity

JF - International Journal of Obesity

SN - 0307-0565

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